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排序方式: 共有643条查询结果,搜索用时 31 毫秒
631.
Phuc Van Pham Ngoc Bich Vu Thuy Thi-Thanh Dao Ha Thi-Ngan Le Lan Thi Phi Oanh Thuy Huynh Mai Thi-Hoang Truong Oanh Thi-Kieu Nguyen Ngoc Kim Phan 《Cytotechnology》2017,69(5):801-814
Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels. 相似文献
632.
Yao D. Cai Yongbo Xue Cindy C. Truong Jose Del Carmen-Li Christopher Ochoa Jens T. Vanselow Katherine A. Murphy Ying H. Li Xianhui Liu Ben L. Kunimoto Haiyan Zheng Caifeng Zhao Yong Zhang Andreas Schlosser Joanna C. Chiu 《Current biology : CB》2021,31(3):502-514.e7
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633.
Superhydrophobic titanium surfaces fabricated by femtosecond laser ablation to mimic the structure of lotus leaves were assessed for their ability to retain coccoid bacteria. Staphylococcus aureus CIP 65.8T, S. aureus ATCC 25923, S. epidermidis ATCC 14990T and Planococcus maritimus KMM 3738 were retained by the surface, to varying degrees. However, each strain was found to preferentially attach to the crevices located between the microscale surface features. The upper regions of the microscale features remained essentially cell-free. It was hypothesised that air entrapped by the topographical features inhibited contact between the cells and the titanium substratum. Synchrotron SAXS revealed that even after immersion for 50 min, nano-sized air bubbles covered 45% of the titanium surface. After 1 h the number of cells of S. aureus CIP 65.8T attached to the lotus-like titanium increased to 1.27 × 105 mm?2, coinciding with the replacement of trapped air by the incubation medium. 相似文献
634.
635.
Dang Quan Nguyen Dinh Hoa Hoang Michael Nelson Lokesh Nigam Vo Thanh Thao Nguyen Lianjun Zhang Tram Kim Thi Pham Huu Duc Ho Dai Dong Thi Nguyen Trung Quoc Lam Trinh To Tat Yasmin Elhajmoussa Quoc Trung Ly Flavia Pichiorri Vinod Pullarkat Bin Zhang Ya-Huei Kuo Guido Marcucci Le Xuan Truong Nguyen 《Journal of cellular physiology》2020,235(10):7567-7579
636.
Shibo Li Hailong Wang Sanaa Jehi Jun Li Shuo Liu Zi Wang Lan Truong Takuya Chiba Zefeng Wang Xiaohua Wu 《The EMBO journal》2021,40(8)
Break‐induced replication (BIR) is a specialized homologous‐recombination pathway for DNA double‐strand break (DSB) repair, which often induces genome instability. In this study, we establish EGFP‐based recombination reporters to systematically study BIR in mammalian cells and demonstrate an important role of human PIF1 helicase in promoting BIR. We show that at endonuclease cleavage sites, PIF1‐dependent BIR is used for homology‐initiated recombination requiring long track DNA synthesis, but not short track gene conversion (STGC). We also show that structure formation‐prone AT‐rich DNA sequences derived from common fragile sites (CFS‐ATs) induce BIR upon replication stress and oncogenic stress, and PCNA‐dependent loading of PIF1 onto collapsed/broken forks is critical for BIR activation. At broken replication forks, even STGC‐mediated repair of double‐ended DSBs depends on POLD3 and PIF1, revealing an unexpected mechanism of BIR activation upon replication stress that differs from the conventional BIR activation model requiring DSB end sensing at endonuclease‐generated breaks. Furthermore, loss of PIF1 is synthetically lethal with loss of FANCM, which is involved in protecting CFS‐ATs. The breast cancer‐associated PIF1 mutant L319P is defective in BIR, suggesting a direct link of BIR to oncogenic processes. 相似文献
637.
638.
Kiet A. Truong Steven L. Stephenson Trung V. Phung Hanh T.M. Tran 《Biotechnology progress》2019,35(6):e2873
Physarum polycephalum is a plasmodial slime mold. One of the trophic stages in the life cycle of this organism is a plasmodium. In submerged culture, plasmodia are fragmented into microplasmodia. The latter both lack cell walls and are capable of rapid growth. There has been limited information on the effects of medium composition on the growth and lipid accumulation of microplasmodia. In this study, optimization of medium components by response surface methodology showed that tryptone and yeast extract concentrations had the most significant effects on lipid and biomass production; significant synergistic interactions between glucose and tryptone concentration on these responses were also recorded. The optimal medium was composed of 20 g/L of glucose, 6.59 g/L of tryptone, and 3.0 g/L of yeast extract. This medium yielded 13.86 g/L of dry biomass and 1.97 g/L of lipids. These amounts are threefold higher than those of the American Type Culture Collection (ATCC) medium. In addition, biomass and lipid production reached maximal values between only 4 and 5 days. Fatty acid compositions analysis by gas chromatography-mass spectrometer (GC–MS) revealed that P. polycephalum lipids consisted mainly of oleic acid (40.5%), linoleic acid (10%), and octadecynoic (15.8%). This is the first report on the fatty acid composition of P. polycephalum microplasmodia. These results suggest that the biomass of microplasmodia could be used as a source of material for direct conversion into biodiesel because of the absence of cell walls or it could also be used as a supplemental source of beneficial fatty acids for humans, albeit with some further evaluation needed. 相似文献
639.
Ngoc-Hong Nguyen Thuc-Huy Duong Huy Truong Nguyen Y Thien Vu Thi-Minh-Dinh Tran Thi-Thanh-Van Ho Chi-Cong Mai Dinh-Tri Mai Hoang-Chuong Nguyen Huong Thuy Le Duc-Dung Pham 《化学与生物多样性》2023,20(7):e202300390
Adenosma bracteosum and Vitex negundo are natural sources of methoxylated flavonoids. Little is known about the α-glucosidase inhibition of multi-methoxylated flavonoid derivatives. Eighteen natural flavonoids were isolated from A. bracteosum and V. negundo. Seven halogenated derivatives were synthesized. Their chemical structures were elucidated by extensive NMR analysis and high-resolution mass spectroscopy as well as comparisons in literature. All compounds were evaluated for their α-glucosidase inhibition. Most compounds showed good activity with IC50 values ranging from 16.7 to 421.8 μM. 6,8-Dibromocatechin was the most active compound with an IC50 value of 16.7 μM. A molecular docking study was conducted, indicating that those compounds are potent α-glucosidase inhibitors. 相似文献
640.