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111.
Sunita Jindal Bendangchuchang Longchar Alka Singh Vikrant Gupta 《Plant signaling & behavior》2015,10(12)
Herein, we report cloning and analysis of promoters of GLABRA2 (AaGL2) homolog and a MIXTA-Like (AaMIXTA-Like1) gene from Artemisia annua. The upstream regulatory regions of AaGL2 and AaMIXTA-Like1 showed the presence of several crucial cis-acting elements. Arabidopsis and A. annua seedlings were transiently transfected with the promoter-GUS constructs using a robust agro-infiltration method. Both AaGL2 and AaMIXTA-Like1 promoters showed GUS expression preferentially in Arabidopsis single-celled trichomes and glandular as well as T-shaped trichomes of A. annua. Transgenic Arabidopsis harboring constructs in which AaGL2 or AaMIXTA-Like1 promoters would control GFP expression, showed fluorescence emanating specifically from trichome cells. Our study provides a fast and efficient method to study trichome-specific expression, and 2 promoters that have potential for targeted metabolic engineering in plants. 相似文献
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Using the Adeno-associated virus (AAV) as a gene
delivery vehicle, we have constructed a recombinant
vector containing the full length rat preproinsulin
gene (vLP-1). Utilizing the well described non-obese
diabetic (NOD) mouse model, an experimental
group (n=10) of animals were intramuscularly (IM)
injected with 107 rAAV virions containing the
insulin gene and compared to a mock-injected control
group (n=10). Blood glucose (glc) was then
measured weekly for 16 weeks. Data showed that
the experimental group contained 70% euglycemic
animals (defined as glc <200mg/dL) versus 10% of
the control animals (P<.05) at 14 weeks. Mean
weight in the treated group was greater than the
untreated group. Insulin mRNA was detected at the
injection site of all of the treated animals, but not
controls. Complete destruction of islets was confirmed
by histology ruling out the possibility of
spontaneous reversal of insulinitis. We conclude
that IM delivery of the insulin gene in the NOD
mouse was able to prevent clinical DM up to 14
weeks in a majority of treated animals. Our experimental
data suggests that gene therapy may be an
alternative treatment for IDDM in the future. 相似文献