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941.
Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton 下载免费PDF全文
Radha Prasanna Balasubramanian Ramakrishnan Kunal Ranjan Siddarthan Venkatachalam Amrita Kanchan Priyanka Solanki Dilip Monga Yashbir S. Shivay Sandhya Kranthi 《Journal of Phytopathology》2016,164(11-12):1030-1042
The suppressive effects of microbial inoculants on cotton seedling mortality were assessed in Rhizoctonia solani‐infested soil. Per cent mortality ranged from 16 to 32 (60–120 days after sowing, DAS) and significant differences were recorded at 120 DAS, especially after drenching with compost tea of Azotobacter sp. and Anabaena torulosa—Trichoderma viride‐biofilmed formulations. The activity of hydrolytic enzymes was reduced in diseased root tissues due to a majority of the microbially inoculated treatments, compared with healthy root tissues. Per cent changes in the amounts of glomalin‐related soil proteins (GRSPs) were 2 to 85% greater than those of the uninoculated experimental controls. These microbial inoculants altered the rhizosphere bacterial communities as evident from the Denaturing gradient gel electrophoresis (DGGE) banding patterns and, also reduced the population of R. solani. While the copy numbers of the internal transcribed spacer (ITS) gene of R. solani in the uninoculated (infested soil) were approximately 1.47 × 1011 per g soil, they were 1.34–1.42 × 105 per g soil after the application of A. torulosa, Anabaena laxa and A. torulosa–Bacillus sp. Increases in yield (ranging from 3 to 23%) due to various microbial inoculants relative to uninoculated controls illustrated their promise as plant growth‐promoting and disease‐suppressing agents. This study illustrates the modulation of rhizosphere ecology through microbial inoculants as a mechanism of disease suppression and sustaining plant growth. 相似文献
942.
Shivani?Nagar V.?P.?SinghEmail author Ajay?Arora Rajkumar?Dhakar Shiv?Ramakrishnan 《Acta Physiologiae Plantarum》2015,37(12):257
Terminal heat stress is one of the limiting factors in wheat production and it is expected to rise under present scenario of climate change. The present study was conducted to evaluate the performance of 40 wheat genotypes under terminal heat stress conditions based on eight physiological traits. The plants were sown late (i.e. on 5th January) to expose them terminal heat stress. The genotypes were evaluated using multivariate analysis viz. Ward’s method of hierarchical clusters analysis, discriminant analysis and principle component analysis. The genotypes were categorized into three groups namely tolerant, intermediate and sensitive. Tolerant genotypes like DBW 14, RAJ 3765, HD 2643 and HALNA performed physiologically better in terms of higher membrane stability index (MSI), chlorophyll content, photosynthesis rate (Pn), harvest index under heat stress conditions. Genotypes HD 2987, SHANGHAI, HD 2402 and WH 730 were found to be heat sensitive. Physiological traits like MSI, SPAD value, Fv/Fm ratio and Pn were found to be most important contributor in grouping of genotypes and showed positive correlations (r) of 0.73, 0.47, 0.41 and 0.39 with grain yield, respectively, which is significant at p < 0.05. The large genetic diversity was found among the genotypes based on physiological traits. These genotypes can be utilized in wheat improvement programme for heat tolerance. 相似文献
943.
Small mammal dispersal is strongly affected by geographical barriers. However, commensal small mammals may be passively transported over large distances and strong barriers by humans (often with agricultural products). This pattern should be especially apparent in topographically complex landscapes, such as mountain ranges, where valleys and/or peaks can limit dispersal of less vagile species. We predict that commensal species would have lower genetic differentiation and higher migration rates than related non-commensals in such landscapes. We contrasted population genetic differentiation in two sympatric Rattus species (R. satarae and R. rattus) in the Western Ghats mountains in southern India. We sampled rats from villages and adjacent forests in seven locations (20–640 km apart). Capture-based statistics confirmed that R. rattus is abundant in human settlements in this region, whereas R. satarae is non-commensal and found mostly in forests. Population structure analyses using ~970-bp mitochondrial control region and 17 microsatellite loci revealed higher differentiation for the non-commensal species (R. satarae F-statistics=0.420, 0.065, R. rattus F-statistics=0.195, 0.034; mitochondrial DNA, microsatellites, respectively). Genetic clustering analyses confirm that clusters in R. satarae are more distinct and less admixed than those in R. rattus. R. satarae shows higher slope for isolation-by-distance compared with R. rattus. Although mode of migration estimates do not strongly suggest higher rates in R. rattus than in R. satarae, they indicate that migration over long distances could still be higher in R. rattus. We suggest that association with humans could drive the observed pattern of differentiation in the commensal R. rattus, consequently impacting not only their dispersal abilities, but also their evolutionary trajectories. 相似文献
944.
An essential role of the basal body protein SAS‐6 in Plasmodium male gamete development and malaria transmission 下载免费PDF全文
Sara R. Marques Chandra Ramakrishnan Raffaella Carzaniga Andrew M. Blagborough Michael J. Delves Arthur M. Talman Robert E. Sinden 《Cellular microbiology》2015,17(2):191-206
Gametocytes are the sole Plasmodium parasite stages that infect mosquitoes; therefore development of functional gametes is required for malaria transmission. Flagellum assembly of the Plasmodium male gamete differs from that of most other eukaryotes in that it is intracytoplasmic but retains a key conserved feature: axonemes assemble from basal bodies. The centriole/basal body protein SAS‐6 normally regulates assembly and duplication of these organelles and its depletion causes severe flagellar/ciliary abnormalities in a diverse array of eukaryotes. Since basal body and flagellum assembly are intimately coupled to male gamete development in Plasmodium, we hypothesized that SAS‐6 disruption may cause gametogenesis defects and perturb transmission. We show that Plasmodium berghei sas6 knockouts display severely abnormal male gametogenesis presenting reduced basal body numbers, axonemal assembly defects and abnormal nuclear allocation. The defects in gametogenesis reduce fertilization and render Pbsas6 knockouts less infectious to mosquitoes. Additionally, we show that lack of Pbsas6 blocks transmission from mosquito to vertebrate host, revealing an additional yet undefined role in ookinete to sporulating oocysts transition. These findings underscore the vulnerability of the basal body/SAS‐6 to malaria transmission blocking interventions. 相似文献
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947.
Winny Routray Sukhinder K. Cheema Vegneshwaran V. Ramakrishnan Julia Pohling 《Critical reviews in biotechnology》2019,39(4):469-488
Microorganisms (microalgae and fungi) are currently the main sources of astaxanthin; however, this carotenoid also accumulates in crustaceans, salmonids, and birds. Seafood (derived from marine animals) processing wastes are significant sources of astaxanthin and can be employed as feed and for nutraceutical applications, where shrimp wastes are the most exploited seafood industry waste employed for astaxanthin extraction. This review discusses different sources, efficient environment-friendly extraction methods employed for astaxanthin extraction, biorefinery approaches for efficient extraction and future aspects of the application of these waste sources for commercial preparation of astaxanthin complexes. It also includes a brief overview of the advantages, disadvantages, and challenges for obtaining astaxanthin from various sources and various case scenarios integrating different biorefinery approaches. 相似文献
948.
Katharina Schiessl Jodi L.S. Lilley Tak Lee Ioannis Tamvakis Wouter Kohlen Paul C. Bailey Aaron Thomas Jakub Luptak Karunakaran Ramakrishnan Matthew D. Carpenter Kirankumar S. Mysore Jiangqi Wen Sebastian Ahnert Veronica A. Grieneisen Giles E.D. Oldroyd 《Current biology : CB》2019,29(21):3657-3668.e5
949.
Shen G Balasubramanian R Wang T Wu Y Hoffart LM Krebs C Bryant DA Golbeck JH 《The Journal of biological chemistry》2007,282(44):31909-31919
950.
Structures of glycoconjugate N-glycans and glycolipids of invertebrates show significant differences from those of vertebrates. These differences are due largely to the vertebrate beta1,4-galactosyltransferase-1 (beta4Gal-T1), which is found as a beta1,4-N-acetylgalactosaminyltransferase (beta4GalNAc-T1) in invertebrates. Mutation of Tyr285 to Ile or Leu in human beta4Gal-T1 converts the enzyme into an equally efficient beta4GalNAc-T1. A comparison of all the human beta4Gal-T1 ortholog enzymes shows that this Tyr285 residue in human beta4Gal-T1 is conserved either as Tyr or Phe in all vertebrate enzymes, while in all invertebrate enzymes it is conserved as an Ile or Leu. We find that mutation of the corresponding Ile residue to Tyr in Drosophila beta4GalNAc-T1 converts the enzyme to a beta4Gal-T1 by reducing its N-acetylgalactosaminyltransferase activity by nearly 1000-fold, while enhancing its galactosyltransferase activity by 80-fold. Furthermore, we find that, similar to the vertebrate/mammalian beta4Gal-T1 enzymes, the wild-type Drosophila beta4GalNAc-T1 enzyme binds to a mammary gland-specific protein, alpha-lactalbumin (alpha-LA). Thus, it would seem that, during the evolution of vertebrates from invertebrates over 500 million years ago, beta4Gal-T1 appeared as a result of the single amino acid substitution of Tyr or Phe for Leu or Ile in the invertebrate beta4GalNAc-T1. Subsequently, the pre-existing alpha-LA-binding site was utilized during mammalian evolution to synthesize lactose in the mammary gland during lactation. 相似文献