全文获取类型
收费全文 | 5801篇 |
免费 | 466篇 |
出版年
2023年 | 17篇 |
2022年 | 39篇 |
2021年 | 102篇 |
2020年 | 59篇 |
2019年 | 84篇 |
2018年 | 93篇 |
2017年 | 104篇 |
2016年 | 125篇 |
2015年 | 244篇 |
2014年 | 272篇 |
2013年 | 397篇 |
2012年 | 463篇 |
2011年 | 456篇 |
2010年 | 324篇 |
2009年 | 276篇 |
2008年 | 392篇 |
2007年 | 404篇 |
2006年 | 373篇 |
2005年 | 328篇 |
2004年 | 324篇 |
2003年 | 281篇 |
2002年 | 284篇 |
2001年 | 72篇 |
2000年 | 62篇 |
1999年 | 65篇 |
1998年 | 70篇 |
1997年 | 48篇 |
1996年 | 37篇 |
1995年 | 34篇 |
1994年 | 27篇 |
1993年 | 24篇 |
1992年 | 37篇 |
1991年 | 22篇 |
1990年 | 31篇 |
1989年 | 22篇 |
1988年 | 30篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 18篇 |
1984年 | 16篇 |
1983年 | 12篇 |
1982年 | 14篇 |
1981年 | 13篇 |
1979年 | 11篇 |
1978年 | 13篇 |
1977年 | 11篇 |
1975年 | 10篇 |
1974年 | 10篇 |
1973年 | 12篇 |
1966年 | 8篇 |
排序方式: 共有6267条查询结果,搜索用时 15 毫秒
81.
Diana Marcela Penarete-Vargas Ana?s Boisson Serge Urbach Hervé Chantelauze Suzanne Peyrottes Laurent Fraisse Henri J. Vial 《PloS one》2014,9(12)
Plasmodium falciparum is responsible for severe malaria which is one of the most prevalent and deadly infectious diseases in the world. The antimalarial therapeutic arsenal is hampered by the onset of resistance to all known pharmacological classes of compounds, so new drugs with novel mechanisms of action are critically needed. Albitiazolium is a clinical antimalarial candidate from a series of choline analogs designed to inhibit plasmodial phospholipid metabolism. Here we developed an original chemical proteomic approach to identify parasite proteins targeted by albitiazolium during their native interaction in living parasites. We designed a bifunctional albitiazolium-derived compound (photoactivable and clickable) to covalently crosslink drug–interacting parasite proteins in situ followed by their isolation via click chemistry reactions. Mass spectrometry analysis of drug–interacting proteins and subsequent clustering on gene ontology terms revealed parasite proteins involved in lipid metabolic activities and, interestingly, also in lipid binding, transport, and vesicular transport functions. In accordance with this, the albitiazolium-derivative was localized in the endoplasmic reticulum and trans-Golgi network of P. falciparum. Importantly, during competitive assays with albitiazolium, the binding of choline/ethanolamine phosphotransferase (the enzyme involved in the last step of phosphatidylcholine synthesis) was substantially displaced, thus confirming the efficiency of this strategy for searching albitiazolium targets. 相似文献
82.
83.
Recent studies have provided evidence for sensory-motor adaptive changes and action goal coding of visually guided manual action in premotor and posterior parietal cortices. To extend these results to orofacial actions, devoid of auditory and visual feedback, we used a repetition suppression paradigm while measuring neural activity with functional magnetic resonance imaging during repeated intransitive and silent lip, jaw and tongue movements. In the motor domain, this paradigm refers to decreased activity in specific neural populations due to repeated motor acts and has been proposed to reflect sensory-motor adaptation. Orofacial movements activated a set of largely overlapping, common brain areas forming a core neural network classically involved in orofacial motor control. Crucially, suppressed neural responses during repeated orofacial actions were specifically observed in the left ventral premotor cortex, the intraparietal sulcus, the inferior parietal lobule and the superior parietal lobule. Since no visual and auditory feedback were provided during orofacial actions, these results suggest somatosensory-motor adaptive control of intransitive and silent orofacial actions in these premotor and parietal regions. 相似文献
84.
85.
86.
87.
88.
89.
Victoire Cardot‐Ruffino Vronique Chauvet Cassandre Caligaris Adrien Bertrand‐Chapel Nicolas Chuvin Roxane M. Pommier Ulrich Valcourt David Vincent Sylvie Martel Sophie Aires Bastien Kaniewski Pierre Dubus Philippe Cassier Stphanie Sentis Laurent Bartholin 《Genesis (New York, N.Y. : 2000)》2020,58(5)
Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1‐Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control of the promoter of Fsp1 (fibroblast‐specific protein 1) gene, whose expression starts after gastrulation at Day 8.5 in cells of mesenchymal origin. We verified the correct expression and function of the Flpo enzyme by several ex vivo and in vivo approaches. The [Fsp1‐Flpo] strain represents a genuine tool to further target the recombination of transgenes with Frt sites specifically in cells of mesenchymal origin or with a fibroblastic phenotype. 相似文献
90.
Fabrizio Gianni Vesna Ma
i Fabrizio Bartolini Alexis Pey Mathieu Laurent Luisa Mangialajo 《Restoration Ecology》2020,28(4):750-756
The role of herbivorous fish in threatening marine forests of temperate seas has been generally overlooked. Only recently, the scientific community has highlighted that high fish herbivory can lead to regime shifts from canopy‐forming algae to less complex turf communities. Here, we present an innovative herbivorous fish deterrent device (DeFish), which can be used for conservation and restoration of marine forests. Compared to most traditional fish exclusion systems, such as cages, the DeFish system does not need regular cleaning and maintenance, making it more cost‐efficient. Resistance of DeFish was tested by installing prototypes at different depths in the French Riviera and in Montenegro: more than 60% of the devices endured several years without maintenance, even if most of them were slightly damaged in the exposed site in Montenegro. The efficacy of DeFish in limiting fish herbivory was tested by an exclusion experiment on Cystoseira amentacea in the French Riviera. In a few months, the number of fish bite marks on the seaweed was decreased, causing a consequent increase in algal length. The device here presented has been conceived for Mediterranean canopy‐forming algae, but the same concept can be applied to other species vulnerable to fish herbivory, such as kelps or seagrasses. In particular, the DeFish design could be improved using more robust and biodegradable materials. Innovative engineering systems, such as DeFish, are expected to become useful tools in the conservation and restoration of marine forests, to complement other practices including active reforestation, herbivore regulation, and regular monitoring of their status. 相似文献