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971.
972.
The Neotropical genus Menevia Schaus, 1928 is revised to include 18 species, 11 of which are new. Two species, Menevia
ostia
comb. n. and Menevia
parostia
comb. n. are transferred from Pamea Walker, 1855 to Menevia. Four species-groups are diagnosed for the first time based on external characters and male genitalia morphology. The following new species are described: Menevia
rosea
sp. n., Menevia
torvamessoria
sp. n., Menevia
magna
sp. n., Menevia
menapia
sp. n., Menevia
mielkei
sp. n., Menevia
australis
sp. n., Menevia
vulgaris
sp. n., Menevia
franclemonti
sp. n., Menevia
vulgaricula
sp. n., Menevia
cordillera
sp. n., and Menevia
delphinus
sp. n.. A neotype is designated for Mimallo
plagiata Walker, 1855, which has since been placed in Menevia. Mimallo
saturata Walker, 1855 is interpreted to be a nomen dubium. 相似文献
973.
Methodological improvements now allow routine analyses of highly degraded DNA samples as found in museum specimens. Using these methods could be useful in studying such groups as rodents of the genus Gerbillus for which i) the taxonomy is still highly debated, ii) collection of fresh specimens may prove difficult. Here we address precise taxonomic questions using a small portion of the cytochrome b gene obtained from 45 dry skin/skull museum samples (from 1913 to 1974) originating from two African and three Asian countries. The specimens were labelled Gerbillus
gerbillus, Gerbillus
andersoni, Gerbillus
nanus, Gerbillus
amoenus, Gerbillus
perpallidus and Gerbillus
pyramidum, and molecular results mostly confirmed these assignations. The close relationship between Gerbillus
nanus (Asian origin) and Gerbillus
amoenus (African origin) confirmed that they represent vicariant sibling species which differentiated in allopatry on either side of the Red Sea. In the closely related Gerbillus
perpallidus and Gerbillus
pyramidum, specimens considered as belonging to one Gerbillus
pyramidum subspecies (Gerbillus
pyramidum
floweri) appeared closer to Gerbillus
perpallidus suggesting that they (Gerbillus
pyramidum
floweri and Gerbillus
perpallidus) may represent a unique species, distributed on both sides of the Nile River, for which the correct name should be Gerbillus
floweri. Furthermore, the three other Gerbillus
pyramidum subspecies grouped together with no apparent genetic structure suggesting that they may not yet represent genetically differentiated lineages. This study confirms the importance of using these methods on museum samples, which can open new perspectives in this particular group as well as in other groups of interest. 相似文献
974.
Comparing concentration‐based (AOT40) and stomatal uptake (PODY) metrics for ozone risk assessment to European forests 下载免费PDF全文
Alessandro Anav Alessandra De Marco Chiara Proietti Andrea Alessandri Alessandro Dell'Aquila Irene Cionni Pierre Friedlingstein Dmitry Khvorostyanov Laurent Menut Elena Paoletti Pierre Sicard Stephen Sitch Marcello Vitale 《Global Change Biology》2016,22(4):1608-1627
Tropospheric ozone (O3) produces harmful effects to forests and crops, leading to a reduction of land carbon assimilation that, consequently, influences the land sink and the crop yield production. To assess the potential negative O3 impacts to vegetation, the European Union uses the Accumulated Ozone over Threshold of 40 ppb (AOT40). This index has been chosen for its simplicity and flexibility in handling different ecosystems as well as for its linear relationships with yield or biomass loss. However, AOT40 does not give any information on the physiological O3 uptake into the leaves since it does not include any environmental constraints to O3 uptake through stomata. Therefore, an index based on stomatal O3 uptake (i.e. PODY), which describes the amount of O3 entering into the leaves, would be more appropriate. Specifically, the PODY metric considers the effects of multiple climatic factors, vegetation characteristics and local and phenological inputs rather than the only atmospheric O3 concentration. For this reason, the use of PODY in the O3 risk assessment for vegetation is becoming recommended. We compare different potential O3 risk assessments based on two methodologies (i.e. AOT40 and stomatal O3 uptake) using a framework of mesoscale models that produces hourly meteorological and O3 data at high spatial resolution (12 km) over Europe for the time period 2000–2005. Results indicate a remarkable spatial and temporal inconsistency between the two indices, suggesting that a new definition of European legislative standard is needed in the near future. Besides, our risk assessment based on AOT40 shows a good consistency compared to both in‐situ data and other model‐based datasets. Conversely, risk assessment based on stomatal O3 uptake shows different spatial patterns compared to other model‐based datasets. This strong inconsistency can be likely related to a different vegetation cover and its associated parameterizations. 相似文献
975.
Fabiana Dal Pozzo Bénédicte Renaville Ludovic Martinelle Robert Renaville Christine Thys Fran?ois Smeets Nathalie Kirschvink Fabien Grégoire Laurent Delooz Guy Czaplicki Claude Saegerman 《Applied and environmental microbiology》2016,82(1):81-86
The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen. 相似文献
976.
Georges St. Laurent Yuri Vyatkin Denis Antonets Maxim Ri Yao Qi Olga Saik Dmitry Shtokalo Michiel?J.L. de?Hoon Hideya Kawaji Masayoshi Itoh Timo Lassmann Erik Arner Alistair R.R. Forrest The FANTOM consortium Estelle Nicolas Timothy A. McCaffrey Piero Carninci Yoshihide Hayashizaki Claes Wahlestedt Philipp Kapranov 《Nucleic acids research》2016,44(7):3233-3252
977.
Evolutionary systematics and biogeography of the arid habitat‐adapted rodent genus Gerbillus (Rodentia,Muridae): a mostly Plio‐Pleistocene African history 下载免费PDF全文
Arame Ndiaye Pascale Chevret Gauthier Dobigny Laurent Granjon 《Journal of Zoological Systematics and Evolutionary Research》2016,54(4):299-317
The systematics of the arid‐adapted Old World Gerbillus rodent genus has always been challenging, with many different taxonomic arrangements proposed. Beyond such taxonomic aspects, the timing and geographical pattern of the evolutionary history of this group remains largely unknown. Based on mitochondrial (cytochrome b) and nuclear (interphotoreceptor retinoid‐binding protein) sequences obtained from the specimens of 21 species, we conducted a phylogenetic analysis of the group, estimated the ages and putative ancestral ranges of its major lineages. Four major clades were clearly retrieved within Gerbillus, for which we propose a subgenus rank. We showed that the emergence of the genus dates back to the end of the Miocene, which corresponds to a period of aridification and C4 vegetation expansion in open habitats, while the four sublineages originated at the end of the Pliocene. Most subsequent diversification events occurred during the Pleistocene, a period characterized by recurrent climatic/environmental shifts with increasing aridification during the last two millions of years. Finally, we suggested that most of the Gerbillus evolutionary history took place in Africa. Only in a few instances did dispersal events from Africa to Asia give birth to extant Asian lineages, a pattern that contrasts with what has been found in many animal groups. 相似文献
978.
Mosley CA Taupenot L Biswas N Taulane JP Olson NH Vaingankar SM Wen G Schork NJ Ziegler MG Mahata SK O'Connor DT 《Biochemistry》2007,46(38):10999-11012
The secretory pro-hormone chromogranin A (CHGA) is densely packed into storage granules along with catecholamines, playing a catalytic role in granule biogenesis. 3-Dimensional structural data on CHGA are lacking. We found a superfamily structural homology for CHGA in the tropomyosin family of alpha-helical coiled-coils, even in mid-molecule regions where primary sequence identity is only modest. The assignment was confirmed by an independent algorithm, suggesting approximately 6-7 such domains spanning CHGA. We provide additional physiochemical evidence (chromatographic, spectral, microscopic) consistent with this unusual structure. Alpha-helical secondary structure (at up to approximately 45%) was confirmed by circular dichroism. CHGA molecular mass was estimated by MALDI-TOF mass spectrometry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, while its native Stokes radius was approximately 84.8 A, as compared to an expected approximately 30 A; the increase gave rise to an apparent native molecular weight of approximately 578 kDa, also consistent with the extended conformation of a coiled-coil. Small-angle X-ray scattering (SAXS) on CHGA in solution best fit an elongated cylindrical conformation in the monodisperse region with a radius of gyration of the rod cross-section (Rt) of approximately 52 A, compatible with a coiled-coil in the hydrated, aqueous state, or a multimeric coiled-coil. Electron microscopy with negative staining revealed an extended, filamentous CHGA structure with a diameter of approximately 94 +/- 4.5 A. Extended, coiled-coil conformation is likely to permit protein "packing" in the secretory granule at approximately 50% higher density than a globular/spherical conformation. Natural allelic variation in the catestatin region was predicted to disrupt the coiled-coil. Chromaffin granule ultrastructure revealed a approximately 108 +/- 6.3 A periodicity of electron density, suggesting nucleation of a binding complex by the CHGA core. Inhibition of CHGA expression, by siRNA, disrupted regulated secretory protein traffic by approximately 65%, while targeted ablation of the CHGA gene in the mouse reduced chromaffin granule cotransmitter concentrations by approximately 40-80%. These results suggest new roles for secretory protein tertiary structure in hormone and transmitter storage, with implications for secretory cargo condensation (or dense core "packing" structure) within the regulated pathway. 相似文献
979.
Peroxisome proliferator-activated receptors (PPARs) (alpha, beta/delta and gamma) are lipid sensors capable of adapting gene expression to integrate various lipid signals. As such, PPARs are also very important pharmaceutical targets, and specific synthetic ligands exist for the different isotypes and are either currently used or hold promises in the treatment of major metabolic disorders. In particular, compounds of the class of the thiazolinediones (TZDs) are PPARgamma agonists and potent insulin-sensitizers. The specific but still broad expression patterns of PPARgamma, as well as its implication in numerous pathways, constitutes also a disadvantage regarding drug administration, since this potentially increases the chance to generate side-effects through the activation of the receptor in tissues or cells not affected by the disease. Actually, numerous side effects associated with the administration of TZDs have been reported. Today, a new generation of PPARgamma modulators is being actively developed to activate the receptor more specifically, in a cell and time-dependent manner, in order to induce a specific subset of target genes only and modulate a restricted number of metabolic pathways. We will discuss here why and how the development of such selective PPARgamma modulators is possible, and summarize the results obtained with the published molecules. 相似文献
980.
Yeasts are often exposed to variations in osmotic pressure in their natural environments or in their substrates when used in fermentation industries. Such changes may lead to cell death or activity loss. Although the involvement of the plasma membrane is strongly suspected, the mechanism remains unclear. Here, the integrity and functionality of the yeast plasma membrane at different levels of dehydration and rehydration during an osmotic treatment were assessed using various fluorescent dyes. Flow cytometry and confocal microscopy of cells stained with oxonol, propidium iodide, and lucifer yellow were used to study changes in membrane polarization, permeabilization, and endocytosis, respectively. Cell volume contraction, reversible depolarization, permeabilization, and endovesicle formation were successively observed with increasing levels of osmotic pressure during dehydration. The maximum survival rate was also detected at a specific rehydration level, of 20 MPa, above which cells were strongly permeabilized. Thus, we show that the two steps of an osmotic treatment, dehydration and rehydration, are both involved in the induction of cell death. Permeabilization of the plasma membranes is the critical event related to cell death. It may result from lipidic phase transitions in the membrane and from variations in the area-to-volume ratio during the osmotic treatment. 相似文献