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991.
George V. Ludwig Lauren C. Iacono-Connors 《In vitro cellular & developmental biology. Animal》1993,29(4):296-309
Summary The Flaviviridae include almost 70 viruses, nearly half of which have been associated with human disease. These viruses are
among the most important arthropod-borne viruses worldwide and include dengue, yellow fever, and Japanese encephalitis viruses.
Morbidity and mortality caused by these viruses vary, but collectively they account for millions of encephalitis, hemorrhagic
fever, arthralgia, rash, and fever cases per year. Most of the members of this family are transmitted between vertebrate hosts
by arthropod vectors, most commonly mosquitoes or ticks. Transmission cycles can be simple or complex depending on the hosts,
vectors, the virus, and the environmental factors affecting both hosts and viruses. Replication of virus in invertebrate hosts
does not seem to result in any significant pathology, which suggests a close evolutionary relationship between virus and vector.
Another example of this relationship is the ability of these viruses to grow in invertebrate cell culture, where replication
usually results in a steady state, persistent infection, often without cytopathic effect. Yields of virus from insect cell
culture vary but are generally similar to yields in vertebrate cells. Replication kinetics are comparable between insect and
vertebrate cell lines, despite differences in incubation temperature. Both vertebrate and insect cell culture systems continue
to play a significant role in flavivirus isolation and the diagnosis of disease caused by these agents. Additionally, these
culture systems permit the study of flavivirus attachment, penetration, replication, and release from cells and have been
instrumental in the production and characterization of live-attenuated vaccines. Both vertebrate and insect cell culture systems
will continue to play a significant role in basic and applied flavivirus research in the future. 相似文献
992.
Stacy A. Krueger-Hadfield Alexis P. Oetterer Lauren E. Lees Jessica M. Hoffman Erik E. Sotka Courtney J. Murren 《Journal of phycology》2023,59(5):926-938
Phenology, or seasonal variation in life cycle events, is poorly described for many macroalgal species. We describe the phenology of a non-native population of Gracilaria vermiculophylla whose thalli are free-living or anchored by decorating polychaetes to tube caps. At a site in South Carolina, USA, we sampled 100 thalli approximately every month from January 2014 to January 2015. We assessed the reproductive state and measured thallus size based on wet weight, thallus length, and thallus surface area from herbarium mounts. Because life cycle stage cannot be assigned using morphology, we implemented a PCR assay to determine the life cycle stage—tetrasporophyte, female gametophyte, or male gametophyte—of each thallus. Tetrasporophytes dominated throughout the year, making up 81%–100% of thalli sampled per month. Reproductive tetrasporophytes varied between 0% and 65% of monthly samples and were most common in warm summer months (July through September) when thalli also tended to be larger. The vast majority of the reproductive thalli were worm-anchored and not fixed to hard substratum via a holdfast. Thus, free-living thalli can be reproductive and potentially seed new non-native populations. Given G. vermiculophylla reproduction seems tied closely to temperature, our work suggests phenology may change with climate-related changes in seawater temperatures. We also highlight the importance of understanding the natural history of macroalgae to better understand the consequence of range expansions on population dynamics. 相似文献
993.
Karen Weisenseel Colin A. Chapman Lauren J. Chapman 《Primates; journal of primatology》1993,34(4):445-450
To evaluate the potential impact of logging on nocturnal primates, densities ofGalago demidovii, G. inustus, andPerodictus potto were determined in two areas of the Kibale Forest Reserve, Uganda. One of these areas had been logged at a moderate level
in the late 1960's, while the second area was relatively undisturbed. The density of the nocturnal primates was lower in the
logged area than in the neighboring unlogged area. For the most frequently sighted nocturnal primate,G. demidovii, sightings were spatially clustered both within a year and between years, suggesting that clumped resources and/or social
factors were influencing space use. 相似文献
994.
Jayashree Srinivasan Anusha Vasudev Carolyn Shasha Hilary J. Selden Encarnacion Perez Jr Bonnie LaFleur Shripad A. Sinari Andreas Krueger Ellen R. Richie Lauren I. R. Ehrlich 《Aging cell》2023,22(8):e13870
Age-related thymus involution results in decreased T-cell production, contributing to increased susceptibility to pathogens and reduced vaccine responsiveness. Elucidating mechanisms underlying thymus involution will inform strategies to restore thymopoiesis with age. The thymus is colonized by circulating bone marrow (BM)-derived thymus seeding progenitors (TSPs) that differentiate into early T-cell progenitors (ETPs). We find that ETP cellularity declines as early as 3 months (3MO) of age in mice. This initial ETP reduction could reflect changes in thymic stromal niches and/or pre-thymic progenitors. Using a multicongenic progenitor transfer approach, we demonstrate that the number of functional TSP/ETP niches does not diminish with age. Instead, the number of pre-thymic lymphoid progenitors in the BM and blood is substantially reduced by 3MO, although their intrinsic ability to seed and differentiate in the thymus is maintained. Additionally, Notch signaling in BM lymphoid progenitors and in ETPs diminishes by 3MO, suggesting reduced niche quality in the BM and thymus contribute to the early decline in ETPs. Together, these findings indicate that diminished BM lymphopoiesis and thymic stromal support contribute to an initial reduction in ETPs in young adulthood, setting the stage for progressive age-associated thymus involution. 相似文献
995.
Julia C. Thierauf Stefan T. Kaluziak Elizabeth Codd Stacy N. Dybel Soma Jobbagy Rashi Purohit Alex A. Farahani Aikaterini Dedeilia Vivek Naranbhai Mai P. Hoang Adam S. Fisch Lauren Ritterhouse Genevieve M. Boland Jochen K. Lennerz A. John Iafrate 《Pigment cell & melanoma research》2023,36(5):378-387
Mucosal melanoma (MM) is a rare subtype of melanoma with an aggressive clinical course. In cutaneous melanoma (CM), the absence of pigmentation and presence of NRAS/KRAS mutations are biomarkers indicating an aggressive clinical course with shorter overall survival. Similar data for MM are missing. We present the real-world outcome data in a cohort of genotyped MM patients and assessed the prognostic relevance of pigmentation- and NRAS/KRAS mutation status. We correlated pathological reports and clinical data with overall survival of patients with MM. Furthermore, we performed clinically integrated molecular genotyping and analyzed real world treatment regimens for covariates associated with clinical outcome. We identified 39 patients with available clinical and molecular data. Patients with amelanotic MM had a significantly shorter overall survival (p = .003). In addition, the presence of a NRAS or KRAS mutation was significantly associated with poor overall survival (NRAS or KRAS p = .024). Currently, it is unknown if the same prognostic relevance for the lack of pigmentation and RAS mutations in CM, exists in MM. Here we analyzed a cohort of MM for outcome measures and determined that two known prognostic biomarkers for CM are in fact novel prognosticators for MM. 相似文献
996.
Yuan-Wei Yan Eddie S Qian Lauren E Woodard Julie Bejoy 《World journal of stem cells》2023,15(6):530-547
Brain diseases affect 1 in 6 people worldwide. These diseases range from acute neurological conditions such as stroke to chronic neurodegenerative disorders such as Alzheimer’s disease. Recent advancements in tissue-engineered brain disease models have overcome many of the different shortcomings associated with the various animal models, tissue culture models, and epidemiologic patient data that are commonly used to study brain disease. One innovative method by which to model human neurological disease is via the directed differentiation of human pluripotent stem cells (hPSCs) to neural lineages including neurons, astrocytes, and oligodendrocytes. Three-dimensional models such as brain organoids have also been derived from hPSCs, offering more physiological relevance due to their incorporation of various cell types. As such, brain organoids can better model the pathophysiology of neural diseases observed in patients. In this review, we will emphasize recent developments in hPSC-based tissue culture models of neurological disorders and how they are being used to create neural disease models. 相似文献
997.
A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (less than 10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro. 相似文献
998.
Lauren M. Cagen Zahir Qureshi Hiroko Nishimura 《Biochemical and biophysical research communications》1983,110(1):250-255
Saline washed red blood cells of the toadfish convert [1-14C] arachidonic acid to products that cochromatograph with prostaglandin E2 and prostaglandin F2α. This synthesis is inhibited by indomethacin (10 μg/ml). Conversion of arachidonic acid to prostaglandin E2 was confirmed by mass spectrometry. When saline washed toadfish red blood cells were incubated with a mixture of [1-14C]-arachidonic acid and [5,6,8,9,11,12,14,15,-3H]-arachidonic acid, comparison of the isotope ratios of the radioactive products indicated that prostaglandin F2α was produced by reduction of prostaglandin E2. The capacity of toadfish red blood cells to reduce prostaglandin E2 to prostaglandin F2α was confirmed by incubation of the cells with [1-14C] prostaglandin E2. 相似文献
999.
Alan Bennett Joan Houghton David J. Leaper Ian F. Stamford 《Prostaglandins & other lipid mediators》1979,17(2):179-191
Adult mice were injected subcutaneously with cells from a syngeneic metastasizing mammary cancer. Daily treatment with flurbiprofen starting before injection of the cancer cells reduced tumour growth and lengthened the survival of mice whose tumours were excised at 3 weeks.When low doses of radiotherapy and chemotherapy were given, additional treatment with flurbiprofen starting 25 days after injecting the cancer cells substantially inhibited tumour growth. 相似文献
1000.
Light-dependent cytochrome P-450 changes in mung beans (Phaseolus aureus). 总被引:1,自引:0,他引:1 下载免费PDF全文
Maximum concentrations of microsomal cytochrome P-450 are present in 3-4 day-old mung beans (Phaseolus aureus). On illumination of dark-grown seedlings, cytochrome P-450 and later cytochrome P-450 undergo a rapid decrease in concentration in vivo, with an apparent half-time of about 6 h. Conversely light-grown seedlings, transferred to darkness, show a slow accumulation of cytochrome P-450, doubling time of about 30 h, with a later accumulation of cytochrome P-420. Microsomal cytochromes b559, b560.5 and b562.5 do not significantly alter on light-dark transitions. Possible functions for dark-induced cytochrome P-450 are discussed. 相似文献