全文获取类型
收费全文 | 17148篇 |
免费 | 1451篇 |
国内免费 | 7篇 |
出版年
2024年 | 12篇 |
2023年 | 118篇 |
2022年 | 160篇 |
2021年 | 535篇 |
2020年 | 309篇 |
2019年 | 401篇 |
2018年 | 459篇 |
2017年 | 353篇 |
2016年 | 664篇 |
2015年 | 1077篇 |
2014年 | 1166篇 |
2013年 | 1351篇 |
2012年 | 1637篇 |
2011年 | 1462篇 |
2010年 | 932篇 |
2009年 | 774篇 |
2008年 | 1023篇 |
2007年 | 997篇 |
2006年 | 891篇 |
2005年 | 809篇 |
2004年 | 775篇 |
2003年 | 620篇 |
2002年 | 621篇 |
2001年 | 107篇 |
2000年 | 96篇 |
1999年 | 113篇 |
1998年 | 128篇 |
1997年 | 97篇 |
1996年 | 90篇 |
1995年 | 67篇 |
1994年 | 66篇 |
1993年 | 53篇 |
1992年 | 53篇 |
1991年 | 46篇 |
1990年 | 40篇 |
1989年 | 51篇 |
1988年 | 42篇 |
1987年 | 28篇 |
1986年 | 22篇 |
1985年 | 31篇 |
1984年 | 30篇 |
1983年 | 25篇 |
1982年 | 22篇 |
1981年 | 22篇 |
1980年 | 21篇 |
1979年 | 22篇 |
1978年 | 17篇 |
1976年 | 19篇 |
1974年 | 17篇 |
1968年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
92.
Laura Perrone Liliana Di Palma Rosario Di Toro Giancarlo Gialanella Renata Moro 《Biological trace element research》1994,41(3):321-330
Concentrations of 8 trace elements (Fe, Cu, Zn, Se, Br, Pb, Rb, and Sr) at different lactation time were measured by the PIXE multi-elemental technique. Time dependence and interelement correlations were studied. A total of 200 milk samples from 32 lactating mothers were supplied from 2 to 120 d after delivery of 26 full-term and 6 preterm infants. All elements showed a lognormal frequency-distribution. The Fe, Cu, Zn, and Se contents in preterm milk were found to be somewhat different with respect to full-term milk. Cu, Zn, Se, Br, Pb, and Rb concentrations declined with lactation time, both in pre- and full-term samples. Sr and Fe contents did not show any change with time. Detailed analysis of data by partial correlation and multiple regression methods was performed. No substantial differences between preterm and full-term samples were found in the results of partial correlation analysis. Cu and Zn were found to be correlated with lactation time, whereas the measured time dependence for the other elements has to be attributed to the effect of the existing interelement correlation. All the measured elements appeared to be correlated with at least one other element. In particular, Se was inversely correlated with Zn and directly with Cu. The zinc and copper contents in milk can therefore depend on the variation in the mother selenium intake. 相似文献
93.
Dispersion and site fidelity of breeding female grey seals (Halichoerus grypus) on North Rona, Scotland 总被引:2,自引:0,他引:2
Female grey seals ( Halichoerus grypus ) formed breeding aggregations on the island of North Rona, Scotland. Aggregations of females were associated particularly with gullies leading from the sea, leaving large areas of available space unoccupied. Changes in the degree of aggregation of females during the breeding season were similar in 1987, 1988 and 1989. Pronounced aggregations occurred in the early and late parts of each breeding season.
Of 67 breeding females marked in 1985, 62 (93%)) returned to N. Rona to breed in at least one season up to 1989, but 18 (27%) were present in all five years. Females came ashore up to 14 days before giving birth and 82% were observed first in the vicinity of their subsequent pupping site. Between 1985 and 1989, marked females which returned were faithful to their previous pupping sites, even when the previous pup had died. There was no evidence of a gradual change in the location of individual pupping sites over time. This pupping site fidelity may generate aggregations whose location, timing and composition is predictable. 相似文献
Of 67 breeding females marked in 1985, 62 (93%)) returned to N. Rona to breed in at least one season up to 1989, but 18 (27%) were present in all five years. Females came ashore up to 14 days before giving birth and 82% were observed first in the vicinity of their subsequent pupping site. Between 1985 and 1989, marked females which returned were faithful to their previous pupping sites, even when the previous pup had died. There was no evidence of a gradual change in the location of individual pupping sites over time. This pupping site fidelity may generate aggregations whose location, timing and composition is predictable. 相似文献
94.
Michael A. Kron Laura Gately Janardan P. Pandey Miguel H. Jurado Jose Rumbea Guzman 《Human genetics》1994,93(5):517-519
Indigenous Indian groups comprise approximately 20% of Ecuador's population, the third largest percentage in all of Central or South America, yet immunogenetic data on these groups are lacking in the literature. In the course of population migration studies, sera collected from 65 Ecuadorians living in the northern province of Esmeraldas were typed for six GM and two KM markers. The study population consisted of 47 Cayapa Indians and 18 blacks of African origin, descendants of slaves imported into the area during the seventeenth century. The Cayapa demonstrated three GM phenotypes, two of which are common to other South American Indian tribes. The frequency of KM1 positive Cayapa Indians (63%) is similar to other South American Indian tribes, but is significantly greater than the Huaorani of eastern Ecuador (2%), the only other Ecuadorian Indian group for whom limited immunoglobulin allotype data are available (
2=35.8, P<0.0001). 相似文献
95.
The human glucagon receptor encoding gene: structure, cDNA sequence and chromosomal localization 总被引:2,自引:0,他引:2
Si Lok Joseph L. Kuijper Laura J. Jelinek Janet M. Kramer Theodore E. Whitmore Cindy A. Sprecher Shannon Mathewes Francis J. Grant Shaula H. Biggs Gary B. Rosenberg Paul O. Sheppard Patrick J. O''Hara Donald C. Foster Wayne Kindsvogel 《Gene》1994,140(2):203-209
Characterization of the human glucagon-receptor-encoding gene (GGR) should provide a greater understanding of blood glucose regulation and may reveal a genetic basis for the pathogenesis of diabetes. A cDNA encoding a complete functional human glucagon receptor (GGR) was isolated from a liver cDNA library by a combination of polymerase chain reaction and colony hybridization. The cDNA encodes a receptor protein with 80% identity to rat GGR that binds [125I] glucagon and transduces a signal leading to increases in the concentration of intracellular cyclic adenosine 3′,5′-monophosphate. Southern blot analysis of human DNA reveals a hybridization pattern consistent with a single GGR locus. In situ hybridization to metaphase chromosome preparations maps the GGR locus to chromosome 17q25. Analysis of the genomic sequence shows that the coding region spans over 5.5 kb and is interrupted by 12 introns. 相似文献
96.
97.
98.
Michael R. Kanost Melissa K. Zepp Noma E. Ladendorff Laura A. Andersson 《Archives of insect biochemistry and physiology》1994,27(2):123-136
A protein that inhibits hemocyte aggregation has been isolated from hemolymph of Manduca sexta larvae and named hemocyte aggregation inhibitor protein (HAIP). HAIP has a Mr = 50,000, pI = 8.5, and contains 7% carbohydrate. It is present at 230 ± 20 μg/ml in hemolymph of day 3 fifth instar larvae. Antibodies to HAIP do not cross-react with M. sexta hemolin, which is similar in size and charge and also inhibits hemocyte aggregation. HAIP and hemolin have some similarity in amino acid composition and NH2-terminal sequence, but are different in overall secondary structure, as determined by CD spectroscopy. The concentration of HAIP in hemolymph is not affected by injection of larvae with bacteria. A protein of approximately 50,000 daltons that reacts with antibody to M. sexta HAIP is present in hemolymph of Bombyx mori, Heliothis zea, and Galleria mellonella. Although the function of HAIP in vivo is not yet clear, it may have a role in modulating adhesion of hemocytes during defensive responses. © 1994 Wiley-Liss, Inc. 相似文献
99.
Even Amler Nelvio Cester Eleonora Salvolini Roberto Staffolani Martin Burkhard Laura Mazzanti Arnot Kotyk Carlo Romanini 《Cell biology international》1994,18(7):723-727
Placentas of women suffering from pregnancy-induced hypertension (PIH) were found to contain a greater amount of Na,K-ATPase molecules, estimated from anthroyl ouabain binding, than normotensive individuals. Both the microsomal fraction of placental cells and purified Na,K-ATPase showed an increased affinity for the specific inhibitor ouabain which, in the case of the microsomes, bound with a dissociation constant of 0.9 nM as compared with 3.4 nM in the controls. Likewise, the dissociation constant of the ouabain complex with purified Na,K-ATPase was about 3.5 times lower in the hypertensive patients. The differences are apparently caused by a different microenvironment of the ouabain-binding site, as reflected in the quantum yield of bound anthroyl ouabain. If an endogenous digitalis-like factor is present in the body fluids to regulate Na,K-ATPase activity, the present results render its role quite plausible. 相似文献
100.
K. Pomeroy D. C. W. Brown Y. Takahata 《In vitro cellular & developmental biology. Plant》1994,30(4):196-203
Summary Microspore-derived embryos fromBrassica napus cv. Topas (low erucic acid) and Reston (high erucic acid) were subjected to treatment with abscisic acid (ABA) during late-stage
embryo development and then dried under controlled relative humidities to mature dry seed levels of moisture. Exogenously
medium-supplied ABA arrested growth and development, reduced moisture content, increased total fatty acids on a dry weight
basis, and stimulated systhesis of proteins in microspore-derived embryos. ABA also resulted in a higher proportion of 22∶1
in cv. Reston (high 22∶1) and increased the level of fatty acid unsaturation in cv. Topas (low 22∶1). The accumulation of
two proteins that co-migrated with cruciferin and napin on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional
gels were also promoted by exposure to ABA, and the degree of accumulation was dependent on the concentration and time of
application of ABA. Controlled desiccation of microspore embryos, used to simulate normal maturation and dehydration of zygotic
embryos during seed development, did not seem to cause an increase of either storage proteins, total fatty acids, or 22∶1
(in cv. Reston), suggesting that dehydration is not a prerequisite for these processes, at least in culturedBrassica embryos. 相似文献