全文获取类型
收费全文 | 17172篇 |
免费 | 1419篇 |
国内免费 | 8篇 |
专业分类
18599篇 |
出版年
2024年 | 21篇 |
2023年 | 130篇 |
2022年 | 305篇 |
2021年 | 533篇 |
2020年 | 309篇 |
2019年 | 399篇 |
2018年 | 457篇 |
2017年 | 352篇 |
2016年 | 662篇 |
2015年 | 1073篇 |
2014年 | 1159篇 |
2013年 | 1344篇 |
2012年 | 1627篇 |
2011年 | 1456篇 |
2010年 | 929篇 |
2009年 | 773篇 |
2008年 | 1019篇 |
2007年 | 995篇 |
2006年 | 886篇 |
2005年 | 807篇 |
2004年 | 772篇 |
2003年 | 618篇 |
2002年 | 619篇 |
2001年 | 105篇 |
2000年 | 90篇 |
1999年 | 110篇 |
1998年 | 125篇 |
1997年 | 97篇 |
1996年 | 90篇 |
1995年 | 66篇 |
1994年 | 60篇 |
1993年 | 49篇 |
1992年 | 49篇 |
1991年 | 39篇 |
1990年 | 38篇 |
1989年 | 49篇 |
1988年 | 39篇 |
1987年 | 24篇 |
1986年 | 20篇 |
1985年 | 28篇 |
1984年 | 30篇 |
1983年 | 22篇 |
1982年 | 19篇 |
1981年 | 18篇 |
1980年 | 21篇 |
1979年 | 19篇 |
1978年 | 13篇 |
1976年 | 17篇 |
1974年 | 13篇 |
1968年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
Laura D. Bertola Laura Tensen Pim van Hooft Paula A. White Carlos A. Driscoll Philipp Henschel Anthony Caragiulo Isabela Dias-Freedman Etotépé A. Sogbohossou Pricelia N. Tumenta Tuqa H. Jirmo Geert R. de Snoo Hans H. de Iongh Klaas Vrieling 《PloS one》2015,10(10)
The evolutionary history of a species is key for understanding the taxonomy and for the design of effective management strategies for species conservation. The knowledge about the phylogenetic position of the lion (Panthera leo) in West/Central Africa is largely based on mitochondrial markers. Previous studies using mtDNA only have shown this region to hold a distinct evolutionary lineage. In addition, anthropogenic factors have led to a strong decline in West/Central African lion numbers, thus, the conservation value of these populations is particularly high. Here, we investigate whether autosomal markers are concordant with previously described phylogeographic patterns, and confirm the unique position of the West/Central African lion. Analysis of 20 microsatellites and 1,454 bp of the mitochondrial DNA in 16 lion populations representing the entire geographic range of the species found congruence in both types of markers, identifying four clusters: 1) West/Central Africa, 2) East Africa, 3) Southern Africa and 4) India. This is not in line with the current taxonomy, as defined by the IUCN, which only recognizes an African and an Asiatic subspecies. There are no indications that genetic diversity in West/Central Africa lions is lower than in either East or Southern Africa, however, given this genetic distinction and the recent declines of lion numbers in this region, we strongly recommend prioritization of conservation projects in West/Central Africa. As the current taxonomic nomenclature does not reflect the evolutionary history of the lion, we suggest that a taxonomic revision of the lion is warranted. 相似文献
993.
Paul Teedon Karen S. Galea Laura MacCalman Kate Jones John Cocker John W. Cherrie Martie van Tongeren 《PloS one》2015,10(8)
A major challenge in biomonitoring studies with members of the general public is ensuring their continued involvement throughout the necessary length of the research. The paper presents evidence on the use of community researchers, recruited from local study areas, as a mechanism for ensuring effective recruitment and retention of farmer and resident participants for a pesticides biomonitoring study. The evidence presented suggests that community researchers’ abilities to build and sustain trusting relationships with participants enhanced the rigour of the study as a result of their on-the-ground responsiveness and flexibility resulting in data collection beyond targets expected. 相似文献
994.
Eeva M. Soininen Gilles Gauthier Frédéric Bilodeau Dominique Berteaux Ludovic Gielly Pierre Taberlet Galina Gussarova Eva Bellemain Kristian Hassel Hans K. Sten?ien Laura Epp Audun Schr?der-Nielsen Christian Brochmann Nigel G. Yoccoz 《PloS one》2015,10(1)
Sympatric species are expected to minimize competition by partitioning resources, especially when these are limited. Herbivores inhabiting the High Arctic in winter are a prime example of a situation where food availability is anticipated to be low, and thus reduced diet overlap is expected. We present here the first assessment of diet overlap of high arctic lemmings during winter based on DNA metabarcoding of feces. In contrast to previous analyses based on microhistology, we found that the diets of both collared (Dicrostonyx groenlandicus) and brown lemmings (Lemmus trimucronatus) on Bylot Island were dominated by Salix while mosses, which were significantly consumed only by the brown lemming, were a relatively minor food item. The most abundant plant taxon, Cassiope tetragona, which alone composes more than 50% of the available plant biomass, was not detected in feces and can thus be considered to be non-food. Most plant taxa that were identified as food items were consumed in proportion to their availability and none were clearly selected for. The resulting high diet overlap, together with a lack of habitat segregation, indicates a high potential for resource competition between the two lemming species. However, Salix is abundant in the winter habitats of lemmings on Bylot Island and the non-Salix portion of the diets differed between the two species. Also, lemming grazing impact on vegetation during winter in the study area is negligible. Hence, it seems likely that the high potential for resource competition predicted between these two species did not translate into actual competition. This illustrates that even in environments with low primary productivity food resources do not necessarily generate strong competition among herbivores. 相似文献
995.
996.
997.
Rebecca A. Drummond Amanda L. Collar Muthulekha Swamydas Carlos A. Rodriguez Jean K. Lim Laura M. Mendez Danielle L. Fink Amy P. Hsu Bing Zhai Hatice Karauzum Constantinos M. Mikelis Stacey R. Rose Elise M. N. Ferre Lynne Yockey Kimberly Lemberg Hye Sun Kuehn Sergio D. Rosenzweig Xin Lin Prashant Chittiboina Sandip K. Datta Thomas H. Belhorn Eric T. Weimer Michelle L. Hernandez Tobias M. Hohl Douglas B. Kuhns Michail S. Lionakis 《PLoS pathogens》2015,11(12)
Candida is the most common human fungal pathogen and causes systemic infections that require neutrophils for effective host defense. Humans deficient in the C-type lectin pathway adaptor protein CARD9 develop spontaneous fungal disease that targets the central nervous system (CNS). However, how CARD9 promotes protective antifungal immunity in the CNS remains unclear. Here, we show that a patient with CARD9 deficiency had impaired neutrophil accumulation and induction of neutrophil-recruiting CXC chemokines in the cerebrospinal fluid despite uncontrolled CNS Candida infection. We phenocopied the human susceptibility in Card9
-/- mice, which develop uncontrolled brain candidiasis with diminished neutrophil accumulation. The induction of neutrophil-recruiting CXC chemokines is significantly impaired in infected Card9
-/- brains, from both myeloid and resident glial cellular sources, whereas cell-intrinsic neutrophil chemotaxis is Card9-independent. Taken together, our data highlight the critical role of CARD9-dependent neutrophil trafficking into the CNS and provide novel insight into the CNS fungal susceptibility of CARD9-deficient humans. 相似文献
998.
Formation of elaborately branched dendrites is necessary for the proper input and connectivity of many sensory neurons. Previous studies have revealed that dendritic growth relies heavily on ER-to-Golgi transport, Golgi outposts and endocytic recycling. How new membrane and associated cargo is delivered from the secretory and endosomal compartments to sites of active dendritic growth, however, remains unknown. Using a candidate-based genetic screen in C. elegans, we have identified the small GTPase RAB-10 as a key regulator of membrane trafficking during dendrite morphogenesis. Loss of rab-10 severely reduced proximal dendritic arborization in the multi-dendritic PVD neuron. RAB-10 acts cell-autonomously in the PVD neuron and localizes to the Golgi and early endosomes. Loss of function mutations of the exocyst complex components exoc-8 and sec-8, which regulate tethering, docking and fusion of transport vesicles at the plasma membrane, also caused proximal dendritic arborization defects and led to the accumulation of intracellular RAB-10 vesicles. In rab-10 and exoc-8 mutants, the trans-membrane proteins DMA-1 and HPO-30, which promote PVD dendrite stabilization and branching, no longer localized strongly to the proximal dendritic membranes and instead were sequestered within intracellular vesicles. Together these results suggest a crucial role for the Rab10 GTPase and the exocyst complex in controlling membrane transport from the secretory and/or endosomal compartments that is required for dendritic growth. 相似文献
999.
Lucia Polletta Enza Vernucci Ilaria Carnevale Tania Arcangeli Dante Rotili Silvia Palmerio Clemens Steegborn Theresa Nowak Mike Schutkowski Laura Pellegrini Luigi Sansone Lidia Villanova Alessandra Runci Bruna Pucci Emanuela Morgante Massimo Fini Antonello Mai Matteo A Russo Marco Tafani 《Autophagy》2015,11(2):253-270
In liver the mitochondrial sirtuin, SIRT5, controls ammonia detoxification by regulating CPS1, the first enzyme of the urea cycle. However, while SIRT5 is ubiquitously expressed, urea cycle and CPS1 are only present in the liver and, to a minor extent, in the kidney. To address the possibility that SIRT5 is involved in ammonia production also in nonliver cells, clones of human breast cancer cell lines MDA-MB-231 and mouse myoblast C2C12, overexpressing or silenced for SIRT5 were produced. Our results show that ammonia production increased in SIRT5-silenced and decreased in SIRT5-overexpressing cells. We also obtained the same ammonia increase when using a new specific inhibitor of SIRT5 called MC3482. SIRT5 regulates ammonia production by controlling glutamine metabolism. In fact, in the mitochondria, glutamine is transformed in glutamate by the enzyme glutaminase, a reaction producing ammonia. We found that SIRT5 and glutaminase coimmunoprecipitated and that SIRT5 inhibition resulted in an increased succinylation of glutaminase. We next determined that autophagy and mitophagy were increased by ammonia by measuring autophagic proteolysis of long-lived proteins, increase of autophagy markers MAP1LC3B, GABARAP, and GABARAPL2, mitophagy markers BNIP3 and the PINK1-PARK2 system as well as mitochondrial morphology and dynamics. We observed that autophagy and mitophagy increased in SIRT5-silenced cells and in WT cells treated with MC3482 and decreased in SIRT5-overexpressing cells. Moreover, glutaminase inhibition or glutamine withdrawal completely prevented autophagy. In conclusion we propose that the role of SIRT5 in nonliver cells is to regulate ammonia production and ammonia-induced autophagy by regulating glutamine metabolism. 相似文献
1000.
Brian W. Haas Michael Brook Laura Remillard Alexandra Ishak Ian W. Anderson Megan M. Filkowski 《PloS one》2015,10(3)
The ability to empathize with other people is a critical component of human social relationships. Empathic processing varies across the human population, however it is currently unclear how personality traits are associated with empathic processing. This study was designed to test the hypothesis that specific personality traits are associated with behavioral and biological indicators of improved empathy. Extraversion and Agreeableness are personality traits designed to measure individual differences in social-cognitive functioning, however each trait-dimension includes elements that represent interpersonal social functioning and elements that do not represent interpersonal social functioning. We tested the prediction that interpersonal elements of Extraversion (Warmth) and Agreeableness (Altruism) are associated with empathy and non-interpersonal elements of Extraversion and Agreeableness are not associated with empathy. We quantified empathic processing behaviorally (empathic accuracy task using video vignettes) and within the brain (fMRI and an emotional perspective taking task) in 50 healthy subjects. Converging evidence shows that highly warm and altruistic people are well skilled in recognizing the emotional states of other people and exhibit greater activity in brain regions important for empathy (temporoparietal junction and medial prefrontal cortex) during emotional perspective taking. A mediation analysis further supported the association between warm-altruistic personality and empathic processing; indicating that one reason why highly warm-altruistic individuals may be skilled empathizers is that they engage the temporoparietal junction and medial prefrontal cortex more. Together, these findings advance the way the behavioral and neural basis of empathy is understood and demonstrates the efficacy of personality scales to measure individual differences in interpersonal social function. 相似文献