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51.
Ravinder K. Kohli Daizy R. Batish H. P. Singh Kuldip S. Dogra 《Biological invasions》2006,8(7):1501-1510
Invasive weeds have threatened the integrity of ecosystems throughout the world. They affect not only the species diversity
of native areas but also their biological integrity. In India, a number of invasive exotic weeds have been reported but some
viz. Parthenium hysterophorus, Lantana camara and Ageratum conyzoides, especially those from tropical America are troublesome and have caused adverse ecological, economic and social impact. These
weeds can be seen growing in different landscapes but are luxuriantly localized in unattended forests and cultivated areas.
Parthenium hysterophorus (Asteraceae, commonly known as congress grass) is perhaps the most troublesome and noxious weed of urban and rural India.
Besides rapidly colonizing areas replacing the native vegetation, it is also known to cause a number of human health problems
such as skin allergy, rhinitis and irritation to eyes of the residents in the vicinity. Likewise, it causes fodder scarcity
in addition to being unpalatable and toxic to livestock. Lantana camara (Verbenaceae), another serious tropical American pest, has encroached upon large areas of land, especially the forests where
it has virtually replaced the forest floor vegetation and reduced tree growth. Also because of its bushy and spreading type
of growth it obstructs forest operations. The third weed, Ageratum conyzoides (Asteraceae, Billy goat weed) has invaded agricultural fields. It interferes with crops and causes yield reductions of major
staple crops of India. When it invades rangeland areas, it out competes native grasses causing scarcity of fodder. These weeds
have similar growth strategies such as fast growth rates, short life-cycles, greater reproductive potential, high competitive
abilities and allelopathy that make them successful invaders of native habitats. Mechanical, chemical, biological and cultural
control tactics have failed individually, though integrated approaches combining all these approaches along with community
participation and proper land management have been relatively successful. This paper presents various aspects of biology,
ecology, hazards and control measures of these weeds. 相似文献
52.
Using an in vitro model, we demonstrate that when CD4 T cells from HIV infected subjects are enriched from total blood lymphocytes the immune response to antigen is augmented. However, augmentation of this response is confined to HIV infected subjects with relatively preserved CD4 T cell counts. Enriching for CD4 T cells had no effect on antigen responses in patients with low CD4 lymphocyte counts. These findings support the concept that CD4 T cells in late stage HIV have inherent qualitative defects. 相似文献
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Dedifferentiation-mediated changes in transposition behavior make the Activator transposon an ideal tool for functional genomics in rice 总被引:1,自引:0,他引:1
Kohli Ajay Prynne Mark Q. Miro Berta Pereira Andy Twyman Richard M. Capell Teresa Christou Paul 《Molecular breeding : new strategies in plant improvement》2004,13(2):177-191
There is an inverse relationship between the level of cytosine methylation in genomic DNA and the activity of plant transposable elements. Increased transpositional activity is seen during early plant development when genomic methylation patterns are first erased and then reset. Prolonging the period of hypomethylation might therefore result in an increased transposition frequency, which would be useful for rapid genome saturation in transposon-tagged plant lines. We tested this hypothesis using transgenic rice plants containing Activator (Ac) from maize. R1 seeds from an Ac-tagged transgenic rice line were either directly germinated and grown to maturity, or induced to dedifferentiate in vitro, resulting in cell lines that were subsequently regenerated into multiple mature plants. Both populations were then analyzed for the presence, active reinsertion and amplification of Ac. Plants from each population showed excision-reinsertion events to both linked and unlinked sites. However, the frequency of transposition in plants regenerated from cell lines was more than nine-fold greater than that observed in plants germinated directly from seeds. Other aspects of transposon behavior were also markedly affected. For example, we observed a significantly larger proportion of transposition events to unlinked sites in cell line-derived plants. The tendency for Ac to insert into transcribed DNA was not affected by dedifferentiation. The differences in Ac activity coincided with a pronounced reduction in the level of genomic cytosine methylation in dedifferentiated cell cultures. We used the differential transposon behavior induced by dedifferentiation in the cell-line derived population for direct applications in functional genomics and validated the approach by recovering Ac insertions in a number of genes. Our results demonstrate that obtaining multiple Ac insertions is useful for functional annotation of the rice genome.These authors contributed equally to the work 相似文献
56.
A specific DNA sequence is required for high frequency of recombination in the ade6 gene of fission yeast. 总被引:11,自引:1,他引:10 下载免费PDF全文
The point mutation M26 in the ade6 gene of Schizosaccharomyces pombe increases recombination frequency by an order of magnitude in comparison with other mutations in the same gene. The hypothesis is tested that this hot spot of recombination requires a specific nucleotide sequence at the M26 site. The DNA sequence is altered systematically by in vitro mutagenesis, and the resulting sequences are introduced into the ade6 gene in vivo by gene replacement. It results that any change of the heptanucleotide ATGACGT leads to loss of high frequency of recombination. Thus this oligonucleotide sequence is necessary for high frequency of recombination, but it seems not to be sufficient. 相似文献
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Sukhmeen?Kaur?Kohli Neha?Handa Anket?Sharma Vandana?Gautam Saroj?Arora Renu?BhardwajEmail author Mohammed?Nasser?Alyemeni Leonard?Wijaya Parvaiz?AhmadEmail author 《Protoplasma》2018,255(1):11-24
The present study demonstrated the combined effect of 24-epibrassinolide and salicylic acid against lead (Pb, 0.25, 0.50, and 0.75 mM) toxicity in Brassica juncea seedlings. Various parameters including water status, metal uptake, total water- and lipid-soluble antioxidants, metal chelator content (total thiols, protein-bound thiols, and non-protein-bound thiols), phenolic compounds (flavonoids, anthocyanins, and polyphenols), and organic acids were studied in 10-day-old seedlings. Dry matter content and the heavy metal tolerance index were reduced by 42.24 and 52.3%, respectively, in response to Pb treatment. Metal uptake, metal-chelating compounds, phenolic compounds, and organic acids were increased in Pb-treated seedlings as compared to control plants. The treatment of Pb-stressed seedlings with combination of EBL and SA resulted in enhancement of heavy metal tolerance index by 40.07%, water content by 1.84%, and relative water content by 23.45%. The total water- and lipid-soluble antioxidants were enhanced by 21.01 and 2.21%, respectively. In contrast, a significant decline in dry weight, metal uptake, thiol, and polyphenol contents was observed following the application of 24-epibrassinolide and salicylic acid. These observations indicate that Pb treatment has an adverse effect on B. juncea seedlings. However, co-application of 24-epibrassinolide and salicylic acid mitigates the negative effects of Pb, by lowering Pb metal uptake and enhancing the heavy metal tolerance index, water content, relative water content, antioxidative capacities, phenolic content, and organic acid levels. 相似文献
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Parthenin and its pyrolysed and photo-derivatives exhibit a range of growth regulatory effects depending on their structure. At 5 mg L-1 (the lowest concentration used) the activity of all parthenin derivatives was greater than that of indole-3-acetic acid (IAA) in biotests based on growth and morphogenetic response of hypocotyl cuttings of Phaseolus aureus. Seedling growth was greatly enhanced by pyrolysed parthenin whereas parthenin and photo-parthenin promoted rooting of the hypocotyl cuttings. Structural changes in parthenin resulting from chemical and photochemical reactions, therefore, alter its growth regulatory action. 相似文献