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排序方式: 共有196条查询结果,搜索用时 15 毫秒
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Using an anti-sense RNA approach, a mouse cell line containing dramatically reduced levels of the 90-kDa heat shock protein (hsp90) has been isolated. This line shows reduced growth at mildly elevated temperatures and reduced survival at highly elevated temperatures but is no more sensitive to herpes simplex virus infection than the parental cells. This is the first direct evidence of a role for an individual mammalian hsp in thermotolerance and suggests that individual hsps may be of different value in the response to various types of stress. 相似文献
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Muqit MM Abou-Sleiman PM Saurin AT Harvey K Gandhi S Deas E Eaton S Payne Smith MD Venner K Matilla A Healy DG Gilks WP Lees AJ Holton J Revesz T Parker PJ Harvey RJ Wood NW Latchman DS 《Journal of neurochemistry》2006,98(1):156-169
Following our identification of PTEN-induced putative kinase 1 (PINK1) gene mutations in PARK6-linked Parkinson's disease (PD), we have recently reported that PINK1 protein localizes to Lewy bodies (LBs) in PD brains. We have used a cellular model system of LBs, namely induction of aggresomes, to determine how a mitochondrial protein, such as PINK1, can localize to aggregates. Using specific polyclonal antibodies, we firstly demonstrated that human PINK1 was cleaved and localized to mitochondria. We demonstrated that, on proteasome inhibition with MG-132, PINK1 and other mitochondrial proteins localized to aggresomes. Ultrastructural studies revealed that the mechanism was linked to the recruitment of intact mitochondria to the aggresome. Fractionation studies of lysates showed that PINK1 cleavage was enhanced by proteasomal stress in vitro and correlated with increased expression of the processed PINK1 protein in PD brain. These observations provide valuable insights into the mechanisms of LB formation in PD that should lead to a better understanding of PD pathogenesis. 相似文献
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Invariant or Type 1 NKT cells (iNKT cells) are a unique population of lymphocytes that share characteristics of T cells and
natural killer (NK) cells. Various studies have shown that positive costimulatory pathways such as the CD28 and CD40 pathways
can influence the expansion and cytokine production by iNKT cells. However, little is understood about the regulation of iNKT
cells by negative costimulatory pathways. Here, we show that in vivo activation with α-GalCer results in increased cytokine
production and expansion of iNKT cells in the absence of programmed cell death ligand-1 (PD-L1, B7-H1, and CD274). To study
whether PD-L1 deficiency on NKT cells would enhance antigen-specific T-cell responses, we utilized CD8+ OT-1 OVA transgenic T cells. α-GalCer enhanced the expansion and cytokine production of OT-1 CD8+ cells after adoptive transfer into wild-type recipients. However, this expansion was significantly enhanced when OT-1 CD8+ T cells were adoptively transferred into PD-L1−/− recipients. To extend these results to a tumor model, we used the B16 melanoma system. PD-L1−/− mice given dendritic cells loaded with antigen and α-GalCer had a significant reduction in tumor growth and this was associated
with increased trafficking of antigen-presenting cells and CD8+ T cells to the tumors. These data demonstrate that abrogating PDL1:PD-1 interactions during the activation of iNKT cells
amplifies an anti-tumor response when coupled with DC vaccination. 相似文献
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Knight RA Chen-Scarabelli C Yuan Z McCauley RB Di Rezze J Scarabelli GM Townsend PA Latchman D Saravolatz L Faggian G Mazzucco A Chowdrey HS Stephanou A Scarabelli TM 《FEBS letters》2008,582(6):984-990
This study evaluates whether cardiac ischemia induces release of urocortin, before and independently from myocyte cell death. Urocortin levels rose after 5-min ischemia and peaked after 10-min ischemia, when cell death was not detected. However, myocyte apoptosis and/or necrosis occurred following 20- and 30-min ischemia, which paralleled a fall in urocortin levels, suggesting that urocortin expression and release are mainly sustained by metabolically challenged, though still viable myocytes. Hence, since cardiac release of urocortin, unlike that of conventional biomarkers, occurs before and apart from cell death, urocortin levels may be clinically useful in the diagnosis of sublethal myocardial ischemia. 相似文献