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71.
Equine infectious anemia (EIA) antigen extracted from the spleen of horses infected with EIA virus was purified by pH treatment, (NH4)2SO4 fractionation and affinity chromatography. The homogeneity of the antigen was indicated by sedimentation rate and sedimentation equilibrium experiments. A S20,w of 0.51 was determined and a molecular weight of 7600 was calculated from sedimentation equilibrium analysis. The amino acid composition of the pure antigen indicated the antigen is an acidic protein. Employing radical immunodiffusion (RID) and pure antigen a method for quantitating antigen content of antigen containing preparations was developed. 相似文献
72.
Philip R. Larson 《American journal of botany》1976,63(3):369-381
The apical 22 cm of a dormant, first-year sprout of Populus grandidentata was sectioned serially, and the primary and secondary xylem systems were studied microscopically and graphically reconstructed. A total of 15 nodes was present on the mature stem and 14 foliar primordia in the dormant bud. The vascular traces in the lower portion of the mature stem conformed to a 2/5 phyllotaxy while those of the upper portion and within the dormant bud conformed to a 3/8 phyllotaxy. The 2/5 to 3/8 phyllotactic transition occurred in an extremely precise and systematic two-step pattern: (1) The lateral traces shifted to a new point of origin on the parent central trace, and (2) three new central traces were initiated in sequence by divergences from left-traces. Metaxylem, when followed downward, conformed to the arrangement of the procambial trace system only within one orthostichy. Below this point, the metaxylem components of lateral traces physically separated from those of the protoxylem and continued downward on a new course. Metaxylem vessels produced by the trace cambium originated from a postulated vessel-generating center at the stem-petiole junction. Each metaxylem vessel developing basipetally through the primary body was continuous with a secondary vessel developing basipetally in the secondary body. Because secondary development closed the vascular cylinder, vessels originating from developing leaves or primordia situated at higher levels in the shoot were displaced radially outward when they entered the secondary xyelm. The distribution of vessels in the secondary xylem can therefore be accounted for by a knowledge of the production and distribution of metaxylem vessels in the primary body. 相似文献
73.
Incorporation of C-photosynthate into major chemical fractions of source and sink leaves of cottonwood 下载免费PDF全文
The incorporation and distribution of photosynthetically fixed 14CO2 was followed for 48 hours in a recently matured source leaf (LPI 7) and in young expanding source and sink leaves (LPI 4) of cottonwood (Populus deltoides Bartr.). The major chemical constituents of leaf laminae and petioles were separated by sequential solvent extractions and enzyme hydrolyses. Two hours after labeling, about 80% of the 14C was found in water-alcohol-soluble constituents in the mature source lamina as compared to about 45% in those of the young expanding leaf. In both mature and expanding source leaves the water-alcohol-soluble constituents decreased while the CHCl3-soluble and -insoluble compounds increased with time. After 48 hours, 7 and 37% of the total 14C was recovered from structural carbohydrates and from protein + CHCl3-soluble fractions, respectively, in the mature source leaf; and 4 and 65%, respectively, in the young source leaf. When the distribution of 14C among major chemical fractions was calculated on per cent dpm/mg basis, the data showed that a young sink leaf incorporated over twice as much 14C into structural carbohydrates as a young source leaf (11% versus 4%). However, when calculated on an absolute dpm/mg basis, activity in this fraction of the young source leaf exceeded that in the sink leaf by a ratio of about 11:1 (9528 versus 845 dpm/mg). Thus, most of the material for synthesis of structural carbohydrates was derived from in situ photosynthate. 相似文献
74.
Stems of cottonwood ( Populs deltoides Bartr. ex Marsh.) plants grown under different conditions were examined to determine the relation between net endogenous auxin yields and the acropetal advance of the primary-secondary vascular transition zone (TZ). In all treatments, the internode yielding maximum net auxin activity, as determined by the Avena curvature bioassay, closely corresponded with the internode in which the TZ occurred. Under short-day (SD) dormancy-inducing conditions, auxin yield declined steadily while the maximum auxin peak and the TZ shifted toward younger internodes. Auxin yields from these plants were extremely low after 5 weeks of SD compared with those from long-day (LD) plants. The only consistent auxin yield was obtained from internodes subtending young leaves beneath the apical bud. Plants placed in SD for 3 weeks and then returned to LD conditions showed an immediate increase in auxin yield in the stem, and the progressive acropetal advance of the TZ under SD was reversed. Therefore, within 7 LD the positions of the TZ and peak auxin yield corresponded to those observed before the imposition of SD Fully dormant plants placed in LD showed a dramatic rise in auxin yields during the first 2 weeks of renewed growth. Although low levels of auxin were found in the newly developing shoots after 6 LD, yields increased rapidly after 9 and 14 LD. The position of the TZ corresponded with the peak of net auxin activity after 9 and 14 LD. 相似文献
75.
76.
Peter T. Euclide Garrett J. McKinney Matthew Bootsma Charlene Tarsa Mariah H. Meek Wesley A. Larson 《Molecular ecology resources》2020,20(1):66-78
Interpretation of high‐throughput sequence data requires an understanding of how decisions made during bioinformatic data processing can influence results. One source of bias that is often cited is PCR clones (or PCR duplicates). PCR clones are common in restriction site‐associated sequencing (RAD‐seq) data sets, which are increasingly being used for molecular ecology. To determine the influence PCR clones and the bioinformatic handling of clones have on genotyping, we evaluate four RAD‐seq data sets. Data sets were compared before and after clones were removed to estimate the number of clones present in RAD‐seq data, quantify how often the presence of clones in a data set causes genotype calls to change compared to when clones were removed, investigate the mechanisms that lead to genotype call changes and test whether clones bias heterozygosity estimates. Our RAD‐seq data sets contained 30%–60% PCR clones, but 95% of RAD‐tags had five or fewer clones. Relatively few genotypes changed once clones were removed (5%–10%), and the vast majority of these changes (98%) were associated with genotypes switching from a called to no‐call state or vice versa. PCR clones had a larger influence on genotype calls in individuals with low read depth but appeared to influence genotype calls at all loci similarly. Removal of PCR clones reduced the number of called genotypes by 2% but had almost no influence on estimates of heterozygosity. As such, while steps should be taken to limit PCR clones during library preparation, PCR clones are likely not a substantial source of bias for most RAD‐seq studies. 相似文献
77.
Background and AimsLeaf functional traits are strongly tied to growth strategies and ecological processes across species, but few efforts have linked intraspecific trait variation to performance across ontogenetic and environmental gradients. Plants are believed to shift towards more resource-conservative traits in stressful environments and as they age. However, uncertainty as to how intraspecific trait variation aligns with plant age and performance in the context of environmental variation may limit our ability to use traits to infer ecological processes at larger scales.MethodsWe measured leaf physiological and morphological traits, canopy volume and flowering effort for Artemisia californica (California sagebrush), a dominant shrub species in the coastal sage scrub community, under conditions of 50, 100 and 150 % ambient precipitation for 3 years.Key ResultsPlant age was a stronger driver of variation in traits and performance than water availability. Older plants demonstrated trait values consistent with a more conservative resource-use strategy, and trait values were less sensitive to drought. Several trait correlations were consistent across years and treatments; for example, plants with high photosynthetic rates tended to have high stomatal conductance, leaf nitrogen concentration and light-use efficiency. However, the trade-off between leaf construction and leaf nitrogen evident in older plants was absent for first-year plants. While few traits correlated with plant growth and flowering effort, we observed a positive correlation between leaf mass per area and performance in some groups of older plants.ConclusionsOverall, our results suggest that trait sensitivity to the environment is most visible during earlier stages of development, after which intraspecific trait variation and relationships may stabilize. While plant age plays a major role in intraspecific trait variation and sensitivity (and thus trait-based inferences), the direct influence of environment on growth and fecundity is just as critical to predicting plant performance in a changing environment. 相似文献
78.
Temperature plays a key role in the biology of ectotherms, including anurans, which are found at higher elevations in the tropics than anywhere in the temperate zone. High elevation tropical environments are characterized by extreme daily thermal fluctuation including high daily maxima and nightly freezing. Our study investigated the contrasting operative temperatures of the anurans Telmatobius marmoratus and Pleurodema marmoratum in different environmental contexts at the same elevation and biome above 5,200 m. Telmatobius marmoratus avoids extremes of daily temperature fluctuation by utilizing thermally buffered aquatic habitat at all life stages, with minimal operative temperature variation (range: 4.6–8.0°C). Pleurodema marmoratum, in contrast, experienced operative temperatures from ?3.5 to 44°C and has one of the widest thermal breadths reported for any tropical frog, from >32°C (critical thermal maximum) to surviving freezing periods of 1 and 6 hr down to ?3.0°C. Our findings expand experimental evidence of frost tolerance in amphibians to the widespread Neotropical family Leptodactylidae, the first such evidence of frost tolerance in a tropical amphibian. Our study identifies three strategies (wide thermal tolerance breadth, use of buffered microhabitats, and behavioral thermoregulation), which allow these tropical frogs to withstand the current wide daily thermal fluctuation above 5,000 m.a.s.l. and which may help them adapt to future climatic changes. Abstract in Spanish is available with online material 相似文献
79.
Jonathan D. Mosley Sara L. Van Driest Emma K. Larkin Peter E. Weeke John S. Witte Quinn S. Wells Jason H. Karnes Yan Guo Lisa Bastarache Lana M. Olson Catherine A. McCarty Jennifer A. Pacheco Gail P. Jarvik David S. Carrell Eric B. Larson David R. Crosslin Iftikhar J. Kullo Gerard Tromp Helena Kuivaniemi David J. Carey Marylyn D. Ritchie Josh C. Denny Dan M. Roden 《PloS one》2013,8(12)
A single mutation can alter cellular and global homeostatic mechanisms and give rise to multiple clinical diseases. We hypothesized that these disease mechanisms could be identified using low minor allele frequency (MAF<0.1) non-synonymous SNPs (nsSNPs) associated with “mechanistic phenotypes”, comprised of collections of related diagnoses. We studied two mechanistic phenotypes: (1) thrombosis, evaluated in a population of 1,655 African Americans; and (2) four groupings of cancer diagnoses, evaluated in 3,009 white European Americans. We tested associations between nsSNPs represented on GWAS platforms and mechanistic phenotypes ascertained from electronic medical records (EMRs), and sought enrichment in functional ontologies across the top-ranked associations. We used a two-step analytic approach whereby nsSNPs were first sorted by the strength of their association with a phenotype. We tested associations using two reverse genetic models and standard additive and recessive models. In the second step, we employed a hypothesis-free ontological enrichment analysis using the sorted nsSNPs to identify functional mechanisms underlying the diagnoses comprising the mechanistic phenotypes. The thrombosis phenotype was solely associated with ontologies related to blood coagulation (Fisher''s p = 0.0001, FDR p = 0.03), driven by the F5, P2RY12 and F2RL2 genes. For the cancer phenotypes, the reverse genetics models were enriched in DNA repair functions (p = 2×10−5, FDR p = 0.03) (POLG/FANCI, SLX4/FANCP, XRCC1, BRCA1, FANCA, CHD1L) while the additive model showed enrichment related to chromatid segregation (p = 4×10−6, FDR p = 0.005) (KIF25, PINX1). We were able to replicate nsSNP associations for POLG/FANCI, BRCA1, FANCA and CHD1L in independent data sets. Mechanism-oriented phenotyping using collections of EMR-derived diagnoses can elucidate fundamental disease mechanisms. 相似文献
80.
Benjamin A. Miko Bevin Cohen Katharine Haxall Laurie Conway Nicole Kelly Dianne Stare Christina Tropiano Allan Gilman Samuel L. Seward Jr Elaine Larson 《PloS one》2013,8(11)