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61.
S. Bermingham P.J. Fisher A. Martin M. Marriott H. Lappin-Scott 《Microbial ecology》1998,35(2):199-204
Abstract
Exposure of the aquatic hyphomycete Heliscus lugdunensis to the herbicide Mecoprop did not significantly affect production of the antigen recognized by the specific monoclonal antibody
NG-CF10. Therefore, an ELISA method, developed in a previous study, could be used to quantify the biomass of H. lugdunensis colonizing leaves exposed to this herbicide. Exposure to Mecoprop significantly reduced the mycelial biomass associated with
alder leaves. This was shown to be a threshold response rather than a dose response, with higher biomass recorded on control
leaves. No significant differences were found over the range of Mecoprop concentrations used.
In laboratory experiments, Gammarus pseudolimnaeus was offered a choice of alder leaves exposed to a range of Mecoprop concentrations. The animals were able to discriminate
between the exposed and control leaves, and between inoculated and sterile leaves. Presence of the fungus resulted in increased
leaf consumption, but no interaction between the Mecoprop concentrations and fungal colonization was observed. The major factor
affecting food choice was the concentration of Mecoprop that the leaves were exposed to—not the Mecoprop-mediated effects
on fungal biomass.
Received: 10 February 1997; Accepted: 8 May 1997 相似文献
62.
Enantioselective degradation of the herbicide mecoprop [2-(2-methyl-4-chlorophenoxy) propionic acid] by mixed and pure bacterial cultures 总被引:6,自引:0,他引:6
Abstract A consortium of three bacteria was isolated from top soil through their capacity to utilise the chlorinated, aromatic herbicide mecoprop as a single growth substrate. The consortium constituted a tight association of Alcaligenes denitrificans, Pseudomonas glycinea and Pseudomonas marginalis . The culture exclusively degraded the ( R )-(+)-isomer of the herbicide while the ( S )-(−)-enantiomer remained unaffected. The mecoprop-degrading community could also degrade 2,4-dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid and racemic 2-phenoxypropionic acid. Initially, no single member of the consortium was able to degrade mecoprop as a pure culture but after prolonged incubation, A. denitrificans was able to grow on the herbicide as the sole source of carbon and energy. 相似文献
63.
Structural deformation of bacterial biofilms caused by short-term fluctuations in fluid shear: an in situ investigation of biofilm rheology. 总被引:10,自引:0,他引:10
P Stoodley Z Lewandowski J D Boyle H M Lappin-Scott 《Biotechnology and bioengineering》1999,65(1):83-92
The physical properties (rheology) of biofilms will determine the shape and mechanical stability of the biofilm structure and consequently affect both mass transfer and detachment processes. Biofilm viscoelasticity is also thought to increase fluid energy losses in pipelines. Yet there is very little information on the rheology of intact biofilms. This is due in part to the difficulty in using conventional testing techniques. The size and nature of biofilms makes them difficult to handle, while removal from a surface destroys the integrity of the sample. We have developed a method which allowed us to conduct simple stress-strain and creep experiments on mixed and pure culture biofilms in situ by observing the structural deformations caused by changes in hydrodynamic shear stress (tau(w)). The biofilms were grown under turbulent pipe flow (flow velocity (u) = 1 m/s, Reynolds number (Re) = 3600, tau(w) = 5. 09 N/m(2)) for between 12 and 23 days. The resulting biofilms were heterogeneous and consisted of filamentous streamers that were readily deformed by changes in tau(w). At tau(w) of 10.11 N/m(2) the streamers were flattened so that the thickness was reduced by 25%. We estimated that the shear modulus (G) of the mixed culture biofilm was 27 N/m(2) and the apparent elastic modulus (E(app)) of both biofilms was in the range of 17 to 40 N/m(2). The biofilms behaved like elastic and viscoelastic solids below the tau(w) at which they were grown but behaved like viscoelastic fluids at elevated tau(w). The implications of these results for fluid energy losses and the processes of mass transfer and detachment are discussed. 相似文献
64.
The use of an automated growth analyser to measure recovery times of single heat-injured Salmonella cells 总被引:2,自引:2,他引:0
P.J. Stephens J.A. Joynson K.W. Davies R. Holbrook H.M. Lappin-Scott & T.J. Humphrey 《Journal of applied microbiology》1997,83(4):445-455
A new approach to the study of recovery times of single heat-injured Salmonella cells isdescribed. It comprises the generation of a standard heat-injured culture, serial dilution of thisculture to near extinction, inoculation of the serial dilutions across many microtitre plates andmeasurement of the subsequent recovery and growth using an automated turbidometric analyser.Lag times for individual cells were estimated from turbidity data using a model that accuratelyextrapolated the growth curve back to the starting inoculum level. Lag times were comparedusing a number of different commercially available pre-enrichment media. The most typical resultwas a very broad distribution of lag times at the single cell inoculum level, with many values inexcess of 20 h. Even at an inoculum level 10-fold higher, lag times for some injured cells wereestimated to be >10 h. More significantly, it was found that some media recovered more injuredcells than others and vice versa. Between the worst and best media there were as many as 3 log10 cycles difference in the number of cells recoverable. No trends were apparent linkingchoice of medium with performance. The implications of these findings, in relation to traditionaland rapid methodology, are discussed. 相似文献
65.
66.
Mohamed M Thabet Thomas WJ Huizinga Désirée M van der Heijde Annette HM van der Helm-van Mil 《Arthritis research & therapy》2009,11(5):1-9
Introduction
Evidence suggests that citrullinated fibrin(ogen) may be a potential in vivo target of anticitrullinated protein/peptide antibodies (ACPA) in rheumatoid arthritis (RA). We compared the diagnostic yield of three enzyme-linked immunosorbent assay (ELISA) tests by using chimeric fibrin/filaggrin citrullinated synthetic peptides (CFFCP1, CFFCP2, CFFCP3) with a commercial CCP2-based test in RA and analyzed their prognostic values in early RA.Methods
Samples from 307 blood donors and patients with RA (322), psoriatic arthritis (133), systemic lupus erythematosus (119), and hepatitis C infection (84) were assayed by using CFFCP- and CCP2-based tests. Autoantibodies also were analyzed at baseline and during a 2-year follow-up in 98 early RA patients to determine their prognostic value.Results
With cutoffs giving 98% specificity for RA versus blood donors, the sensitivity was 72.1% for CFFCP1, 78.0% for CFFCP2, 71.4% for CFFCP3, and 73.9% for CCP2, with positive predictive values greater than 97% in all cases. CFFCP sensitivity in RA increased to 80.4% without losing specificity when positivity was considered as any positive anti-CFFCP status. Specificity of the three CFFCP tests versus other rheumatic populations was high (> 90%) and similar to those for the CCP2. In early RA, CFFCP1 best identified patients with a poor radiographic outcome. Radiographic progression was faster in the small subgroup of CCP2-negative and CFFCP1-positive patients than in those negative for both autoantibodies. CFFCP antibodies decreased after 1 year, but without any correlation with changes in disease activity.Conclusions
CFFCP-based assays are highly sensitive and specific for RA. Early RA patients with anti-CFFCP1 antibodies, including CCP2-negative patients, show greater radiographic progression. 相似文献67.
An extra layer of complexity in the regulation of gene expression in bacteria is now apparent through previously unanticipated
roles of noncoding and antisense RNAs. 相似文献
68.
Franks A Egan S Holmström C James S Lappin-Scott H Kjelleberg S 《Applied and environmental microbiology》2006,72(9):6079-6087
The marine epiphytic bacterium Pseudoalteromonas tunicata produces a range of extracellular secondary metabolites that inhibit an array of common fouling organisms, including fungi. In this study, we test the hypothesis that the ability to inhibit fungi provides P. tunicata with an advantage during colonization of a surface. Studies on a transposon-generated antifungal-deficient mutant of P. tunicata, FM3, indicated that a long-chain fatty acid-coenzyme A ligase is involved in the production of a broad-range antifungal compound by P. tunicata. Flow cell experiments demonstrated that production of an antifungal compound provided P. tunicata with a competitive advantage against a marine yeast isolate during surface colonization. This compound enabled P. tunicata to disrupt an already established fungal biofilm by decreasing the number of yeast cells attached to the surface by 66% +/- 9%. For in vivo experiments, the wild-type and FM3 strains of P. tunicata were used to inoculate the surface of the green alga Ulva australis. Double-gradient denaturing gradient gel electrophoresis analysis revealed that after 48 h, the wild-type P. tunicata had outcompeted the surface-associated fungal community, whereas the antifungal-deficient mutant had no effect on the fungal community. Our data suggest that P. tunicata is an effective competitor against fungal surface communities in the marine environment. 相似文献
69.
70.
Yoshifumi Kotani Shoji Shinkai Hiroshi Okamatsu Masamichi Toba Kishiko Ogawa Hiroto Yoshida Taro Fukaya Yoshinori Fujiwara Paulo HM Chaves Keiji Kakumoto Noriyuki Kohda 《Immunity & ageing : I & A》2010,7(1):1-11