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41.
Alessandro Negro Vincenza Corsa Giuseppe Corona Claudio Grandi Stephen D. Skaper Lanfranco Callegaro 《Neurochemical research》1994,19(2):223-227
Ciliary neurotrophic factor (CNTF) is a polypeptide that promotes the survival and/or differentiation of a number of neural cell types. Here we present a structural and functional analysis of the human CNTF molecule. Variant proteins were synthesized byEscherichia coli trnsformmed with mutant cDNA constructs, and purified by SDS-polyacrylamide gel electrophoresis and reverse phase high pressure liquid chromatography. Most variant CNTF proteins lacked neurotrophic activity, but two N-and C-terminal deletions (2–14 and 173–200, respectively) actually displayed a several-fold increase in specific activity. Loss of biological activity was accompanied by changes in the alphahelical nature of CNTF as measured by circular dichroism. These data strengthen the proposed similarity between CNTF and the family of hematopoietic cytokines. 相似文献
42.
Alessandro Di Martino Jiri Safar Lanfranco Callegaro Norman Salem Jr. Clarence J. Gibbs Jr. 《Neurochemical research》1993,18(8):907-913
Ganglioside composition in brains of terminally ill LVG/LAK golden Syrian hamsters infected with the 263K strain of the scrapie agent was analyzed. Results were compared to those obtained from noninfected animals matched by age, sex, and strain. Gangliosides extracted from scrapie-infected animals showed little change in major components, while an increased number of new alkali-labile species appeared. Additionally, the animal strain employed demonstrated a significant polymorphism in brain ganglioside composition. No significant changes in incubation time, clinical development or pathologic features of scrapie were associated with this polymorphism. 相似文献
43.
Giuseppe Arienti Gian C. Di Renzo Ermelando V. Cosmi Enrico Carlini Lanfranco Corazzi 《Neurochemical research》1993,18(3):335-338
The fluorescence anisotropy (r) of diphenylhexatriene (DPH) and of trimethylamino-diphenylhexatriene (TMA-DPH) as a function of temperature (10° to 54°C) was measured in brain microsomes of newborn rats prenatally exposed to ethanol. In this temperature range, the relationship between r and T was linear. The addition of ethanol in vitro to microsomal suspensions influenced the slope of the line of r versus T only when DPH was used as a probe and with high concentrations of the alcohol (0.3 M).The administration of ethanol (18% of total energy intake) in vivo to pregnant dams affected the slope of the lines of r versus T of the microsomes of pups, either using DPH or TMA-DPH as probes. The slope was also affected in brain microsomes obtained from dams, yet, only with TMA-DPH and in the opposite sense than in pups. We conclude that the effect of prenatal exposure to ethanol depended on metabolic alterations induced by the alcohol and not on its detergent properties for the following reasons: (a) The effects in vitro and in vivo were different and (b) in vitro effects could be obtained only with high concentrations (0.3 M), whereas in vivo effects were produced by small doses of ethanol. Besides, the effects of the administration of the alcohol in vivo were different in adult and intrauterine life.Abbreviations DPH
1,6-diphenyl hexa-1,3,5-triene
- HEPES
4-(2-hydroxyethyl-1-piperazineethansulfonic) acid
- SHB
sucrose-HEPES-buffer (0.32 M sucrose, 2 mM HEPES, pH 7.0)
- TMA-DPH
1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene, p-toluensulfonate 相似文献
44.
Transport of Phosphatidylserine from Microsomes to the Inner Mitochondrial Membrane in Brain Tissue 总被引:1,自引:0,他引:1
Lanfranco Corazzi Roberto Pistolesi Enrico Carlini Giuseppe Arienti 《Journal of neurochemistry》1993,60(1):50-56
Abstract: Phosphatidylserine was labeled by incubating rat brain homogenates with [3-14 C]serine in the presence of Ca2+ (base-exchange conditions). Some labeled phosphati-dylethanolamine also forms, in spite of the inhibition of Ca2+ on phosphatidylserine decarboxylase. Phosphatidylserine labeling and decarboxylation also occur on incubating a mixture of purified mitochondria and microsomes, suggesting that no soluble factors are necessary for the synthesis and the decarboxylation of phosphatidylserine. Ca2+ favors the transfer of phosphatidylserine from microsomes (where it forms) to mitochondria (where it is decarboxylated). The specific radioactivity of the phosphatidylserine transferred to mitochondria is higher than that of microsomal phosphatidylserine. This finding supports the hypothesis that the lipid is compartmentalized in microsomes and that radioactive, newly synthesized phosphatidylserine is much better exported than the bulk of microsomal phospholipid. 相似文献
45.
46.
Marisa Manzano Luca Cocolin Barbara Citterio Lanfranco Conte Marco de Bertoldi Giuseppe Comi Gianfranco Santovito Mariano Beltramini Benedetto Salvato 《Biometals》2000,13(3):251-259
A strain of Candida famata was adapted to high copper concentration (1.26 mM) and a number of biochemical parameters have been tested, in order to get information on the mechanisms of metal toxicity and detoxification as well as on the metabolic responses to the treatment. The cytoplasmic levels of superoxide dismutase, peroxidase and glutathione were found significantly increased with respect to control cells, in contrast to catalase which is not affected. The activities of enolase and of triosephosphate isomerase are found to decrease as a consequence of the exposure to copper. Statistically significant differences in the content of some aminoacids are found between copper-treated and control cells. 相似文献
47.
The authors studied the activity of acid and alkaline phosphatase in macrophages of Tenebrio molitor larvae stimulated with various types of asbestos: A and B chrysotile, crocidolite, amosite and anthophylite. The activity of the two enzymes increased, as did those of beta-galactosidase and N-acetyl-beta-D-glucosaminidase, two previously assayed enzymes. The increase indicates the toxic action of various types of asbestos and correlates to variations in the mortality curves. The increases of the enzymatic activity and the macrophage response vary with the type of asbestos. 相似文献
48.
Marco A. Minetto Alberto Botter Sebastjan Šprager Fiorenza Agosti Alessandra Patrizi Fabio Lanfranco Alessandro Sartorio 《Journal of electromyography and kinesiology》2013,23(2):285-295
The aims of this study were to examine if surface EMG signals can be detected from the quadriceps femoris muscle of severely obese patients and to investigate if differences exist in quadriceps force and myoelectric manifestations of fatigue between obese patients and lean controls.Fourteen severely obese patients (body mass index, BMI, mean ± SD: 44.9 ± 6.3 kg/m2) and fourteen healthy controls (BMI: 23.7 ± 2.5 kg/m2) were studied. The vastus medialis and lateralis of the dominant thigh were concurrently investigated during voluntary isometric contractions (10-s long at submaximal and maximal intensities and intermittent submaximal contractions until exhaustion) and sustained (120-s long) electrically elicited contractions.We found that the detection of surface EMG signals from the quadriceps is feasible also in severely obese subjects presenting increased thickness of the subcutaneous fat tissue. In addition, we confirmed and extended previous findings showing that the volume conductor properties determine the amplitude and spectral features of the detected surface EMG signals: the lower the subcutaneous tissue thickness, the higher the amplitude and mean frequency estimates. Further, we found no differences in the mechanical and myoelectric manifestations of fatigue during intermittent voluntary and sustained electrically elicited contractions between obese patients and lean controls. 相似文献
49.
We recently found that ethanol-induced long-term facilitation (LTF) of NMDAR activity is mediated by NR2B-NMDARs and is observed in the dorsomedial striatum (DMS) but not in the dorsolateral striatum (DLS). We also showed that repeated administration of ethanol causes a long-lasting increase in NMDAR activity in the DMS, resulting from ethanol-mediated Fyn phosphorylation of NR2B subunits. In this addendum, we report that the different sensitivity of NMDARs to ethanol between the DMS and DLS is not attributed to the abundance of synaptic NR2B-NMDARs or differences in Fyn levels. We further show that LTF is specific for NR2B-, but not NR2A-NMDARs, and that the duration of the in vivo ethanol-mediated increase in NMDAR activity is associated with the period of ethanol exposure, but not with alteration in NR1 or NR2A protein levels. Together, these results suggest that upregulation of NR2B-NMDAR activity by ethanol is selective and that ethanol's effect on NMDAR activity is gradual and cumulative. 相似文献
50.
Loretta?MancinelliEmail author Teresa?Secca Paula?M?De Angelis Francesco?Mancini Matteo?Marchesini Cristiano?Marinelli Lanfranco?Barberini Francesco?Grignani 《Cell division》2013,8(1):11