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101.
102.
Summary Quantitative measurements of apical growth, nuclear movements and pseudo-clamp connection formation were compared with photomicroscopic details of cytoplasmic events in live A-mutant hyphae of Schizophyllum commune. Nuclear behavior was described during pseudo-clamp connection formation in uninucleate, binucleate and trinucleate hyphal apices and intercalation was shown in sub-terminal regions of these hyphae. Conventional (i.e. rearward)rd) pseudo-clamp connection formation was contrasted with forward pseudo-clamp initiation. Primary branches were shown to be initiated from uninucleate, septate pseudo-clamps. The ultrastructural aspects of A-mutant septa were delineated. 相似文献
103.
Conformational properties and thermodynamics of the RNA duplex r(CGCAAAUUUGCG)2: comparison with the DNA analogue d(CGCAAATTTGCG)2. 下载免费PDF全文
The thermodynamic stability of nine dodecamers (four DNA and five RNA) of the same base composition has been compared by UV-melting. TheDeltaG of stabilisation were in the order: r(GACUGAUCAGUC)2>r(CGCAAATTTGCG)2 approximately r(CGCAUAUAUGCG)2>d(CGCAAATTTGCG)2 approximately r(CGCAAAUUUGCG)2>d(CGCATATATGCG)2 approximately d(GACTGATCAGTC)2>r(CGCUUUAAAGCG)2 approximately d(CGCTTTAAAGCG)2. Compared with the mixed sequences, both r(AAAUUU) and r(UUUAAA) are greatly destablising in RNA, whereas in DNA, d(TTTAAA) is destabilising but d(AAATTT) is stabilising, which has been attributed to the formation of a special B'structure involving large propeller twists of the A-T base pairs. The solution structure of the RNA dodecamer r(CGCAAAUUUGCG)2has been determined using NMR and restrained molecular dynamics calculations to assess the conformational reasons for its stability in comparison with d(CGCAAATTTGCG)2. The structures refined to a mean pairwise r.m.s.d. of 0.89+/-0.29 A. The nucleotide conformations are typical of the A family of structures. However, although the helix axis displacement is approximately 4.6 A into the major groove, the rise (3.0 A) and base inclination ( approximately 6 degrees ) are different from standard A form RNA. The extensive base-stacking found in the AAATTT tract of the DNA homologue that is largely responsible for the higher thermodynamic stability of the DNA duplex is reduced in the RNA structure, which may account for its low relative stability. 相似文献
104.
The distribution of wildlife parasites in a landscape is intimately tied to the spatial distribution of hosts. In parasite
species, including many gastrointestinal parasites, with obligate or common environmental life stages, the dynamics of the
parasite can also be strongly affected by geophysical components of the environment. This is especially salient in host species,
for example humans and macaques, which thrive across a wide variety of habitat types and quality and so are exposed to a wealth
of environmentally resilient parasites. Here, we examine the effect of environmental and anthropogenic components of the landscape
on the prevalence, intensity, and species diversity of gastrointestinal parasites across a metapopulation of long-tailed macaques
on the island of Bali, Indonesia. Using principal-components analysis, we identified significant interaction effects between
specific environmental and anthropogenic components of the landscape, parsing the Balinese landscape into anthropogenic (PC1),
mixed environment (PC2), and non-anthropogenic (PC3) components. Further, we determined that the anthropogenic environment
can mitigate the prevalence and intensity of specific gut parasites and the intensity of the overall community of gut parasites,
but that non-anthropogenically driven landscape components have no significant effect in increasing or reducing the intensity
or prevalence of the community of gut parasites in Balinese macaques. 相似文献
105.
106.
107.
Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. 总被引:20,自引:10,他引:20 下载免费PDF全文
P J Romaniuk B Zoltowska T J Trust D J Lane G J Olsen N R Pace D A Stahl 《Journal of bacteriology》1987,169(5):2137-2141
Comparison of partial 16S rRNA sequences from representative Campylobacter species indicates that the Campylobacter species form a previously undescribed basic eubacterial group, which is related to the other major groups only by very deep branching. This analysis was extended to include the spiral bacterium associated with human gastritis, Campylobacter pylori (formerly Campylobacter pyloridis). The distance between C. pylori and the other Campylobacter species is sufficient to exclude the pyloric organism from the Campylobacter genus. The results indicate that C. pylori is more closely related to Wolinella succinogenes than it is to the other Campylobacter species inspected. Another close relative of the campylobacters was found to be Thiovulum, a sulfide-dependent marine bacterium. 相似文献
108.
Characterization of peptides cleaved by plasmin from the C-terminal polymerization domain of human fibrinogen 总被引:4,自引:0,他引:4
C Southan E Thompson M Panico T Etienne H R Morris D A Lane 《The Journal of biological chemistry》1985,260(24):13095-13101
The C-terminal region of the fibrinogen gamma chain is known to participate in several functional interactions including fibrin polymerization. This part of the molecule is retained on the gamma chain of fragment D (FgD) when fibrinogen is digested by plasmin in the presence of calcium to produce the fragment D-fragment E (FgD X FgE) complex but is lost if FgD is prepared in the absence of calcium. In an attempt to characterize the C-terminal polymerization domain we have used three techniques to examine this further degradation of FgD following the addition of EDTA and plasmin. Analysis of the digestion by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a progressive cleavage of the gamma chain to two small remnants. The polymerization-inhibitory activity of the whole digest was studied using acid-solubilized fibrin. A progressive loss of inhibitory activity was associated with gamma chain shortening, reaching greater than a 120-fold reduction at the end of digestion. The cleavage of peptides was followed by reverse-phase high performance liquid chromatography and the release of a characteristic peptide triplet was associated with gamma chain cleavage. Manual sequencing, amino acid analysis, and fast atom bombardment mass spectrometry established the three peptides as gamma 303-356, 357-373, and 374-405. These peptides have sequences in common with those peptides recently reported by other investigators to be potent polymerization inhibitors. However, when a mixture of the three peptides was added in a 200-fold molar excess to polymerizing fibrin, no inhibitory activity could be demonstrated. It is concluded that the C-terminal polymerization domain of fibrinogen may be an extended region which includes the sequence gamma 303-405, when this is contiguous with the remainder of the gamma chain. 相似文献
109.
Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. 总被引:97,自引:17,他引:97 下载免费PDF全文
Point mutations in the p53 gene are the most frequently identified genetic change in human cancer. They convert murine p53 from a tumour suppressor gene into a dominant transforming oncogene able to immortalize primary cells and bring about full transformation in combination with an activated ras gene. In both the human and murine systems the mutations lie in regions of p53 conserved from man to Xenopus. We have developed a monoclonal antibody to p53 designated PAb240 which does not immunoprecipitate wild type p53. A series of different p53 mutants all react more strongly with PAb240 than with PAb246. The PAb240 reactive form of p53 cannot bind to SV40 large T antigen but does bind to HSP70. In contrast, the PAb246 form binds to T antigen but not to HSP70. PAb240 recognizes all forms of p53 when they are denatured. It reacts with all mammalian p53 and chicken p53 in immunoblots. We propose that immunoprecipitation of p53 by PAb240 is diagnostic of mutation in both murine and human systems and suggest that the different point mutations which convert p53 from a recessive to a dominant oncogene exert a common conformational effect on the protein. This conformational change abolishes T antigen binding and promotes self-oligomerization. These results are consistent with a dominant negative model where mutant p53 protein binds to and neutralizes the activity of p53 in the wild type conformation. 相似文献
110.
The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens. 下载免费PDF全文
D C Pallas W Weller S Jaspers T B Miller W S Lane T M Roberts 《Journal of virology》1992,66(2):886-893