海巴戟ACO基因启动子的克隆及功能初步验证

为了进一步明确海巴戟(Morinda citrifolia Linn.)ACO基因(GenBank登录号:ARB05681.1)功能,本研究通过染色体步移法克隆得到2 066 bp启动子.该区域除了含有大量TATA-box、CAAT-box等启动子核心元件外,还含有乙烯等植物激素响应元件、胁迫和防御相关元件、光相关元件...     

Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2

Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the e...     

基因枪介导甘蔗遗传转化几个影响因素的研究

用基因枪GJ1000介导将Bt基因转入甘蔗嫩叶、Ⅰ型愈伤组织和Ⅱ型愈伤组织。结果表明,用Ⅱ型愈伤组织作为受体最用利于转化;气体压力、轰击距离、轰击次数和真空度对转化效率有不同程度的影响。条件优化后,得到了大量的抗性愈伤组织和一些抗性植株,转化率分别为34.9％和3.36％。     

人类肥胖基因瘦素蛋白的原核表达

目的：原核表达人类肥胖基因瘦素蛋白,方法：以携人类肥胖基因的pUC119-ob为模拟,PCR扩增瘦素蛋白基因片段,并克隆到pET-32a构建重组表达质粒pET-32a-ob,经酶切和测序鉴定后,转化至大肠埃希菌DH5α中表达,SDS-PAGE电泳鉴定表达产物。结果：测序和限制性分析均证明了pET-32a-ob的序列正确,转化的DH5α可高效表达一个30kD融合蛋白,与预期结果一致。结论：经pET-32a-ob转化的DH5α可有效表达重组人类瘦素蛋白,为进一步研究瘦素蛋白的生物活性提供了基础。     

《蛛形学报》(1992～2001年)学术论文计量分析

采用文献计量的方法分析了《蛛形学报》（1992～2001)发表的学术论文、刊物特色、作者地区、单位分布,引文概况,并就进一步推动《蛛形学报》的发展提出了建议。     

Distribution of amiloride-sensitive sodium channels in the oral cavity of the hamster

The distribution of amiloride-sensitive sodium channels (ASSCs) in taste buds isolated from the oral cavity of hamsters was assessed by patch clamp recording. In contrast to the case for rats, taste cells from the fungiform, foliate and vallate papillae and from the soft palate all contain functional ASSCs. The differential distribution of ASSCs between the hamster and the rat may be important for understanding the physiology underlying the differing behavioral responses of these species to sodium salts.      

中国南芥属新分类群

多年生草本,高5-18厘米。根状茎圆锥状,淡黄色。基生叶丛生,呈莲座状,肉质,多数,倒卵形,长2-3厘米,宽5-8毫米,顶端钝或锐尖,边缘被密睫毛或具疏齿,两面无毛;叶柄具窄翅,长1-2厘米,边缘被短毛;茎生叶1-3枚,狭披针形,长约2.5厘米,宽约5毫米,顶端渐尖,全缘,基部呈叶耳,半抱茎。     

Predicting Cortical Bone Strength from DXA and Dental Cone-Beam CT

Objective

This study compared the capabilities of dual-energy X-ray absorptiometry (DXA) and dental cone-beam computed tomography (CBCT) for predicting the cortical bone strength of rat femurs and tibias.

Materials and Methods

Specimens of femurs and tibias obtained from 14 rats were first scanned with DXA to obtain the areal bone mineral density (BMD) of the midshaft cortical portion of the bones. The bones were then scanned using dental CBCT to measure the volumetric cortical bone mineral density (vCtBMD) and the cross-sectional moment of inertia (CSMI) for calculating the bone strength index (BSI). A three-point bending test was conducted to measure the fracture load of each femur and tibia. Bivariate linear Pearson analysis was used to calculate the correlation coefficients (r values) among the CBCT measurements, DXA measurements, and three-point bending parameters.

Results

The correlation coefficients for the associations of the fracture load with areal BMD (measured using DXA), vCtBMD (measured using CBCT), CSMI (measured using CBCT), and BSI were 0.585 (p = 0.028) and 0.532 (p = 0.050) (for the femur and tibia, respectively), 0.638 (p = 0.014) and 0.762 (p = 0.002), 0.778 (p = 0.001) and 0.792 (p<0.001), and 0.822 (p<0.001) and 0.842 (p<0.001), respectively.

Conclusions

CBCT was found to be superior to DXA for predicting cortical bone fracture loads in rat femurs and tibias. The BSI, which is a combined index of densitometric and geometric parameters, was especially useful. Further clinical studies are needed to validate the predictive value of BSI obtained from CBCT and should include testing on human cadaver specimens.