全文获取类型
收费全文 | 126篇 |
免费 | 13篇 |
国内免费 | 1篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 10篇 |
2014年 | 8篇 |
2013年 | 15篇 |
2012年 | 13篇 |
2011年 | 11篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 7篇 |
2007年 | 4篇 |
2006年 | 1篇 |
2005年 | 3篇 |
2004年 | 6篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有140条查询结果,搜索用时 15 毫秒
51.
52.
Reduction in Exopolysaccharide Viscosity as an Aid to Bacteriophage Penetration through Pseudomonas aeruginosa Biofilms 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Geoffrey W. Hanlon Stephen P. Denyer Cedric J. Olliff Lamia J. Ibrahim 《Applied microbiology》2001,67(6):2746-2753
To cause an infection, bacteriophages must penetrate the alginate exopolysaccharide of Pseudomonas aeruginosa to reach the bacterial surface. Despite a lack of intrinsic motility, phage were shown to diffuse through alginate gels at alginate concentrations up to 8% (wt/vol) and to bring about a 2-log reduction in the cell numbers in 20-day-old biofilms of P. aeruginosa. The inability of alginate to act as a more effective diffusional barrier suggests that phage may cause a reduction in the viscosity of the exopolysaccharide. Samples (n = 5) of commercial alginate and purified cystic fibrosis (CF) alginate were incubated with 2 × 108 purified phage per ml for 24 h at 37°C. After incubation the samples and controls were subjected to rheological analysis with a Carrimed controlled stress rheometer. The viscosities of phage-treated samples were reduced by up to 40% compared to those of controls incubated in the absence of phage. The experiment was repeated by using phage concentrations of 1010 and 1012 phage per ml and samples taken for analysis at intervals up to 4 h. The results indicated that there was a time- and concentration-dependent reduction in viscosity of up to 40% compared to the viscosities of the controls. Commercial and purified CF alginate samples, both phage treated and untreated, were subjected to gel filtration chromatography by using Sephacryl High Resolution S-400 medium in order to obtain evidence of degradation. The results demonstrated that alginate treated with phage had a lower molecular weight than untreated alginate. The data suggest that bacteriophage migration through P. aeruginosa biofilms may be facilitated by a reduction in alginate viscosity brought about by enzymic degradation and that the source of the enzyme may be the bacterial host itself. 相似文献
53.
Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl, a commercial egg yolk extender 总被引:6,自引:0,他引:6
Amirat L Tainturier D Jeanneau L Thorin C Gérard O Courtens JL Anton M 《Theriogenology》2004,61(5):895-907
Low-density lipoproteins (LDL) have been previously isolated and identified as the cryoprotective fraction of yolk. The effect of LDL on sperm motility after freezing-thawing has been reported, but no study has been made to assess the effect of LDL on bull semen fertility. The aim of this study was to evaluate the fertility of bull semen cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Optidyl, a commercial extender containing egg yolk. To evaluate the fertilizing ability of semen, we used in vitro fertilization test, whereas acrosome and plasma membrane integrity were also evaluated. The percentage of motile spermatozoa was two fold higher after freezing in LDL than in Optidyl 54.4% versus 30.2% (P < 0.05). The cleavage rate was significantly higher after fertilization with semen frozen in LDL than with Optidyl 63.0% versus 54.8% (P < 0.05). No significant difference was observed on the blastocyst rate after in vitro culture. Integrity of the acrosome and the plasma membrane were maintained in both extenders. In conclusion, LDL preserve bull semen quality and fertilizing ability, allowing also better semen motility, after the freeze-thaw process. 相似文献
54.
Imen Ben-Mustapha Meriem Ben-Ali Najla Mekki Etienne Patin Christine Harmant Jihène Bouguila Houda Elloumi-Zghal Abdelaziz Harbi Mohamed Béjaoui Lamia Boughammoura Jalel Chemli Mohamed-Ridha Barbouche 《Immunogenetics》2014,66(1):67-71
Mendelian susceptibility to mycobacterial disease (MSMD) is a rare disorder predisposing apparently healthy individuals to infections caused by weakly virulent mycobacteria such as bacille Calmette–Guerin (BCG), environmental mycobacteria, and poorly virulent Salmonella strains. IL-12p40 deficiency is the first reported human disease due to a cytokine gene defect and is one of the deficiencies that cause MSMD. Nine mutant alleles only have been identified in the IL12B gene, and three of them are recurrent mutations due to a founder effect in specific populations. IL-12p40 deficiency has been identified especially in countries where consanguinity is high and where BCG vaccination at birth is universal. We investigated, in such settings, the clinical, cellular, and molecular features of six IL-12p40-deficient Tunisian patients having the same mutation in IL12B gene (c.298_305del). We found that this mutation is inherited as a common founder mutation arousing ~1,100 years ago. This finding facilitates the development of a preventive approach by genetic counseling and prenatal diagnosis especially in affected families. 相似文献
55.
Lamia Hila Hédia Tébourbi Leila Abaied Imène Rejeb Lamia Ben Jemaa Habiba Chaabouni 《Biochemical genetics》2009,47(9-10):727-733
Subtelomeric rearrangements significantly contribute to idiopathic mental retardation and result in several mental retardation syndromes; however, most subtelomeric defects lack a characteristic phenotype. Thirty patients with unexplained mental retardation, a normal R banded karyotype at the 550 band, and no clinically recognizable syndrome were screened by Multiplex ligation-dependent probe amplification (MLPA). Four anomalies were identified: deletion 17q, duplications (4q), and associated duplications 15q and Xq. This duplication was found in two sisters of the proband. Anomalies were unidentified by the conventional technique. The prevalence of subtelomeric imbalances in our cohort of moderate to severe mental retardation is around 13% and is consistent with the literature. The sensitivity of the MLPA technique was characterized on cytogenetically verified positive and negative controls. MLPA is a fast, reliable, and relatively inexpensive technique to detect subtelomeric rearrangement in comparison with the fluorescence in situ hybridization (FISH) technique. 相似文献
56.
Fathia Aouidi Hana Gannoun Nada Ben Othman Lamia Ayed Moktar Hamdi 《Process Biochemistry》2009,44(5):597-601
The effect of the cheese whey's (CW) addition on the fermentative decolorization of olive mill wastewater (OMW) by Lactobacillus paracasei, with and without pH adjustment by lime, was investigated. Mixtures OMW/CW at different proportions were fermented. The highest colour removal (47%) and total phenolic reduction (22.7%) of OMW were obtained after cofermentation of OMW/CW at proportions of 10/90, respectively. The decrease of pH after cofermentation of the two wastewaters, induced the precipitation of whey proteins with phenolic compounds and, so, improves decolorization. These removal yields reached 64% and 34%, respectively after precipitation by adjustment of pH at 7 with lime at the end of cofermentation. These improvements were correlated to a clarification of wastewaters by precipitation of whey proteins with phenolic compounds. An enhanced decolorization (up to 93%) and a total phenolic reduction (50%) of the mixture were obtained when cofermentation sequentially pH corrected by lime addition was investigated. 相似文献
57.
The effects of water potential on germination were studied in four provenances of Myrtus from different bioclimatic zones in Tunisia. Water potential between 0 and −10 bars were obtained using polyethylene glycol
6000 (PEG-6000) solutions. Seeds were planted in Petri dishes full of sterile perlite, and kept for one month under 1500 lux
light with 16h photoperiod and at 25°C. A low water potential induced a reduction in germination percentage and germination
speed. Significant variations between the provenances were found even under control conditions, especially at the speed germination
level. The provenances from humid regions were the most susceptible to water stress, and their germination percentage showed
significant reductions at −8 bars. These differences in germination ability of provenances might be attributed to intraspecific
variations resulting from the effects of natural selection and genetic pool background. 相似文献
58.
L Oulmi A Gorlas G Gimenez C Robert A Boulahrouf D Raoult V Roux 《Journal of bacteriology》2012,194(19):5482-5483
A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. 相似文献
59.
Ben Dhiab Rym Ghenim Nejeh Trabelsi Lamia Yahia Ali Challouf Rafika Ghozzi Khemissa Ammar Jihene Omrane Hela Ben Ouada Hatem 《Journal of applied phycology》2010,22(6):745-752
Combined effect of light intensity and glucose concentration on Arthrospira platensis growth and photosynthetic response was evaluated using a 32 factorial design. This design was carried out with light levels of 50, 100, and 150 μmol photons m−2 s−1 and glucose concentrations of 0.5, 1.5, and 2.5 g L−1. Results from the response surface methodology were that the highest level of light intensity and glucose concentration improved
biomass (1.33 g L−1), maximum specific growth rate (0.49 day−1), and net photosynthetic rate (139.89 μmol O2 mg Chl−1 h−1). Furthermore, the interaction of both factors showed that at low light, glucose had a low effect on maximum biomass and
maximal net photosynthetic rate. However, at the highest light levels, the effect of glucose was more sensitive and the increase
of glucose concentration increased the levels of all responses. The rates of the instantaneous relative growth, net photosynthesis,
and dark respiration of growth cultures showed two different phases in mixotrophic condition. The first was distinguished
by the preponderance of the photoautotrophic mode; the second was based mainly on photoheterotrophy. 相似文献
60.
Mkaouar-Rebai Emna Ammar Marwa Sfaihi Lamia Alila-Fersi Olfa Maalej Marwa Felhi Rahma Hachicha Mongia Fakhfakh Faiza 《Molecular biology reports》2021,48(5):4373-4382
Molecular Biology Reports - Mitochondrial diseases include a wide group of clinically heterogeneous disorders caused by a dysfunction of the mitochondrial respiratory chain and can be related to... 相似文献