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941.
Surface-associated swarming motility is implicated in enhanced bacterial spreading and virulence, hence it follows that anti-swarming effectors could have clinical benefits. When investigating potential applications of anti-swarming materials it is important to consider whether the lack of swarming corresponds with an enhanced sessile biofilm lifestyle and resistance to antibiotics. In this study, well-defined tannins present in multiple plant materials (tannic acid (TA) and epigallocathecin gallate (EGCG)) and undefined cranberry powder (CP) were found to block swarming motility and enhance biofilm formation and resistance to tobramycin in Pseudomonas aeruginosa. In contrast, gallic acid (GA) did not completely block swarming motility and did not affect biofilm formation or tobramycin resistance. These data support the theory that nutritional conditions can elicit an inverse relationship between swarming motility and biofilm formation capacities. Although anti-swarmers exhibit the potential to yield clinical benefits, it is important to be aware of possible implications regarding biofilm formation and antibiotic resistance. 相似文献
942.
Doan Thanh Lam Le Sandrine Zanna Isabelle Frateur Philippe Marcus Pascal Loubière 《Biofouling》2013,29(5):479-490
This work was devoted to probe, at the entire population level, interactions between mucins and Lactococcus lactis, using QCM-D. Real-time monitoring of adsorption on polystyrene of PGM (Pig Gastric Mucin) and subsequent adhesion of L. lactis was performed for IBB477 and MG1820 strains. Measuring simultaneously shifts in resonance frequency and dissipation on the polystyrene-coated crystal demonstrated a two-phase process for PGM adsorption. XPS analysis confirmed the presence of adsorbed mucin. The Voigt-based model was used to describe the QCM-D outputs. The predicted thickness of the PGM layer was consistent with the AFM experimental value. Adhesion of L. lactis to bare or PGM-coated polystyrene was then monitored, in combination with DAPI cell counting. Positive frequency shifts were caused by adhering bacteria. The presence of adsorbed PGM strongly reduced bacterial adhesion. However, adhesion of IBB477 to the PGM coating was greatly increased in comparison with that of MG1820. Muco-adhesion may be a highly variable and valuable phenotypic trait among L. lactis strains. 相似文献
943.
Diana P. Pires Sónia Silva Carina Almeida Mariana Henriques Erin M. Anderson Joseph S. Lam 《Biofouling》2013,29(10):1169-1180
Pseudomonas aeruginosa and Candida albicans are disparate microbial species, but both are known to be opportunistic pathogens frequently associated with nosocomial infections. The aim of this study was to provide a better understanding of the interactions between these microorganisms in dual-species biofilms. Several bacteriophage-resistant P. aeruginosa phenotypes have been isolated and were used in dual-species mixed-biofilm studies. Twenty-four and 48 h mixed-biofilms were formed using the isolated phenotypes of phage-resistant P. aeruginosa and these were compared with similar experiments using other P. aeruginosa strains with a defined lipopolysaccharide (LPS) deficiency based on chromosomal knockout of specific LPS biosynthetic genes. Overall, the results showed that the variants of phage-resistant P. aeruginosa and LPS mutants were both less effective in inhibiting the growth of C. albicans in mixed-biofilms compared to the wild-type strains of P. aeruginosa. Conversely, the proliferation of P. aeruginosa was not influenced by the presence of C. albicans. In conclusion, the ability of strains of P. aeruginosa to inhibit the formation of a biofilm of C. albicans appears to be correlated with the LPS chain lengths of phenotypes of P. aeruginosa, suggesting that LPS has a suppressive effect on the growth of C. albicans. 相似文献
944.
Shi Y Qu J Zhang D Zhao P Zhang Q Tam PO Sun L Zuo X Zhou X Xiao X Hu J Li Y Cai L Liu X Lu F Liao S Chen B He F Gong B Lin H Ma S Cheng J Zhang J Chen Y Zhao F Yang X Chen Y Yang C Lam DS Li X Shi F Wu Z Lin Y Yang J Li S Ren Y Xue A Fan Y Li D Pang CP Zhang X Yang Z 《American journal of human genetics》2011,(6):438-813
High myopia, which is extremely prevalent in the Chinese population, is one of the leading causes of blindness in the world. Genetic factors play a critical role in the development of the condition. To identify the genetic variants associated with high myopia in the Han Chinese, we conducted a genome-wide association study (GWAS) of 493,947 SNPs in 1088 individuals (419 cases and 669 controls) from a Han Chinese cohort and followed up on signals that were associated with p < 1.0 × 10−4 in three independent cohorts (combined, 2803 cases and 5642 controls). We identified a significant association between high myopia and a variant at 13q12.12 (rs9318086, combined p = 1.91 × 10−16, heterozygous odds ratio = 1.32, and homozygous odds ratio = 1.64). Furthermore, five additional SNPs (rs9510902, rs3794338, rs1886970, rs7325450, and rs7331047) in the same linkage disequilibrium (LD) block with rs9318086 also proved to be significantly associated with high myopia in the Han Chinese population; p values ranged from 5.46 × 10−11 to 6.16 × 10−16. This associated locus contains three genes—MIPEP, C1QTNF9B-AS1, and C1QTNF9B. MIPEP and C1QTNF9B were found to be expressed in the retina and retinal pigment epithelium (RPE) and are more likely than C1QTNF9B-AS1 to be associated with high myopia given the evidence of retinal signaling that controls eye growth. Our results suggest that the variants at 13q12.12 are associated with high myopia. 相似文献
945.
Lam le T Böhm SV Roberts RG Morris GE 《Biochemical and biophysical research communications》2011,(2):291-295
The nuclear envelope-associated cytoskeletal protein, nesprin-2, is encoded by a large gene containing several internal promoters that produce shorter isoforms. In a study of Ntera-2 teratocarcinoma cells, a novel isoform, nesprin-2-epsilon, was found to be the major mRNA and protein product of the nesprin-2 gene. Its existence was predicted by bioinformatic analysis, but this is the first direct demonstration of both the mRNA and the 120 kDa protein which is located at the nuclear envelope. In a panel of 21 adult and foetal human tissues, the nesprin-2-epsilon mRNA was strongly expressed in ovary but was a minor isoform elsewhere. The expression pattern suggests a possible link with very early development and a likely physiological role in ovary. 相似文献
946.
Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and generally considered to be a saprophyte, but it is
also an important opportunistic human pathogen. Pseudomonas aeruginosa elaborates a variety of virulence factors, one of which is lipopolysaccharide (LPS). LPS of P. aeruginosa is composed of three distinct regions: lipid A, core oligosaccharide (OS), and the long-chain O antigen. The core OS of P. aeruginosa is composed of L-glycero-D-manno-heptose, 3-deoxy-D-manno-oct-2-ulosonic acid, D-galactosamine, D-glucose, and L-rhamnose. Noncarbohydrate substituents are also found in the core
OS including phosphate, 2-aminoethyl (di)phosphate, acetyl, alanyl and carbamoyl groups. Pseudomonas aeruginosa simultaneously synthesizes two core glycoforms, namely, capped and uncapped core. The capped core is covalently attached
to an O antigen, whereas the uncapped core is devoid of O antigen. Although the core of P. aeruginosa LPS is relatively conserved, strain-to-strain variability of its structure exists. This includes phosphorylation pattern,
the level of O-acetylation, and the presence or absence of a fourth glucose residue at the distal end of the uncapped core.
A number of studies have been reported on the structures of unique truncated core OS with unusual modifications. This mini-review
summarizes the diversity of P. aeruginosa complete and truncated core OS structures published over the past fifteen years 相似文献
947.
Rosalie van Zelm Raquel de Paiva Seroa da Motta Wan Yee Lam Wilbert Menkveld Eddie Broeders 《Journal of Industrial Ecology》2020,24(4):913-922
In the light of a circular economy, the Nijhuis Ammonia Recovery system (AECO‐NAR) was developed to not only remove nitrogen from wastewater streams, but also produce ammonium sulfate (AS), used as fertilizer, in a single plant. The goal of this paper was to quantify the environmental impacts of side stream ammonia recovery with the AECO‐NAR system and compares them with the impacts of side stream nitrogen removal combined SHARON (partly nitrification)‐anammox plant. For this, an environmental life cycle assessment was performed with a functional unit (FU) of the treatment of 1 kg of total dissolved nitrogen inflow. Since AS obtained by the AECO‐NAR is a by‐product of the ammonia removal process, allocation was based on system expansion. Foreground inventory data were obtained from a full‐scale plant. ReCiPe2016 was used to determine human health and biodiversity impacts. Results show that due to the production of AS in an integrated water treatment and production system, the AECO‐NAR avoids impacts of current AS production, leading to negative impact scores. Impacts per FU decrease with increasing inflow concentrations of ammonia. Main improvement options are the use of renewable energy and the replacement of the cleaning chemical citric acid with a sustainable alternative. Total impacts of the AECO‐NAR system diminish when comparing the system to the biological SHARON‐Anammox system, due to production of AS fertilizer product. Due to the fertilizer production step being integrated in the side stream treatment, the complete system is beneficial over ammonia recovery and wastewater treatment as separate systems. 相似文献
948.
When DNA sequences from Bermuda plants described as Codium isthmocladum ssp. clavatum and a recent collection from Florida originally thought to be C. decorticatum were analyzed, they were found to be a genetic match to the Pacific Mexican species C. simulans. Historical voucher collections assigned to C. isthmocladum ssp. clavatum show that this Pacific lineage has been present in Bermuda (Atlantic) waters for over a century, thus precluding a very recent introduction from the Pacific. We hypothesize that C. simulans may have hitched a ride on the ballast rocks of early commercial ships plying their trade between the Pacific and Atlantic Oceans. 相似文献
949.
Simultaneous quantification of N2, NH3 and N2O emissions from a flooded paddy field under different N fertilization regimes 总被引:1,自引:0,他引:1
Longlong Xia Xiaobo Li Qianqian Ma Shu Kee Lam Benjamin Wolf Ralf Kiese Klaus Butterbach‐Bahl Deli Chen Zhian Li Xiaoyuan Yan 《Global Change Biology》2020,26(4):2292-2303
Gaseous nitrogen (N) emissions, especially emissions of dinitrogen (N2) and ammonia (NH3), have long been considered as the major pathways of N loss from flooded rice paddies. However, no studies have simultaneously evaluated the overall response of gaseous N losses to improved N fertilization practices due to the difficulties to directly measure N2 emissions from paddy soils. We simultaneously quantified emissions of N2 (using membrane inlet mass spectrometry), NH3 and nitrous oxide (N2O) from a flooded paddy field in southern China over an entire rice‐growing season. Our field experiment included three treatments: a control treatment (no N addition) and two N fertilizer (220 kg N/ha) application methods, the traditional surface application of N fertilizer and the incorporation of N fertilizer into the soil. Our results show that over the rice‐growing season, the cumulative gaseous N losses from the surface application treatment accounted for 13.5% (N2), 19.1% (NH3), 0.2% (N2O) and 32.8% (total gaseous N loss) of the applied N fertilizer. Compared with the surface application treatment, the incorporation of N fertilizer into the soil decreased the emissions of NH3, N2 and N2O by 14.2%, 13.3% and 42.5%, respectively. Overall, the incorporation of N fertilizer into the soil significantly reduced the total gaseous N loss by 13.8%, improved the fertilizer N use efficiency by 14.4%, increased the rice yield by 13.9% and reduced the gaseous N loss intensity (gaseous N loss/rice yield) by 24.3%. Our results indicate that the incorporation of N fertilizer into the soil is an effective agricultural management practice in ensuring food security and environmental sustainability in flooded paddy ecosystems. 相似文献
950.
Bao Quoc Lam Sushant K. Shrivastava Anju Shrivastava Sharmila Shankar Rakesh K. Srivastava 《Journal of cellular and molecular medicine》2020,24(14):7706-7716
The incidence of obesity and type 2 diabetes (T2DM) in the Western world has increased dramatically during the recent decades. According to the American Cancer Society, pancreatic cancer (PC) is the fourth leading cause of cancer‐related death in the United States. The relationship among obesity, T2DM and PC is complex. Due to increase in obesity, diabetes, alcohol consumption and sedentary lifestyle, the mortality due to PC is expected to rise significantly by year 2040. The underlying mechanisms by which diabetes and obesity contribute to pancreatic tumorigenesis are not well understood. Furthermore, metabolism and microenvironment within the pancreas can also modulate pancreatic carcinogenesis. The risk of PC on a population level may be reduced by modifiable lifestyle risk factors. In this review, the interactions of diabetes and obesity to PC development were summarized, and novel strategies for the prevention and treatment of diabetes and PC were discussed. 相似文献