全文获取类型
收费全文 | 965篇 |
免费 | 74篇 |
专业分类
1039篇 |
出版年
2023年 | 8篇 |
2022年 | 17篇 |
2021年 | 25篇 |
2020年 | 19篇 |
2019年 | 14篇 |
2018年 | 12篇 |
2017年 | 28篇 |
2016年 | 30篇 |
2015年 | 29篇 |
2014年 | 52篇 |
2013年 | 55篇 |
2012年 | 62篇 |
2011年 | 66篇 |
2010年 | 38篇 |
2009年 | 35篇 |
2008年 | 48篇 |
2007年 | 42篇 |
2006年 | 36篇 |
2005年 | 28篇 |
2004年 | 48篇 |
2003年 | 29篇 |
2002年 | 21篇 |
2001年 | 19篇 |
2000年 | 28篇 |
1999年 | 30篇 |
1998年 | 13篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 7篇 |
1991年 | 13篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 11篇 |
1987年 | 10篇 |
1986年 | 6篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 7篇 |
1981年 | 6篇 |
1979年 | 10篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 10篇 |
1974年 | 13篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1970年 | 7篇 |
1969年 | 3篇 |
排序方式: 共有1039条查询结果,搜索用时 15 毫秒
41.
42.
Dinesh Mondal Murari Lal Das Vijay Kumar M. Mamun Huda Pradeep Das Debashis Ghosh Jyoti Priyanka Greg Matlashewski Axel Kroeger Alexander Upfill-Brown Rajib Chowdhury 《PLoS neglected tropical diseases》2016,10(8)
BackgroundWe investigated the efficacy, safety and cost of lime wash of household walls plus treatment of sand fly breeding places with bleach (i.e. environmental management or EM), insecticide impregnated durable wall lining (DWL), and bed net impregnation with slow release insecticide (ITN) for sand fly control in the Indian sub-continent.MethodsThis multi-country cluster randomized controlled trial had 24 clusters in each three sites with eight clusters per high, medium or low sand fly density stratum. Every cluster included 45–50 households. Five households from each cluster were randomly selected for entomological measurements including sand fly density and mortality at one, three, nine and twelve months post intervention. Household interviews were conducted for socioeconomic information and intervention acceptability assessment. Cost for each intervention was calculated. There was a control group without intervention.FindingsSand fly mortality [mean and 95%CI] ranged from 84% (81%-87%) at one month to 74% (71%-78%) at 12 months for DWL, 75% (71%-79%) at one month to 49% (43%-55%) at twelve months for ITN, and 44% (34%-53%) at one month to 22% (14%-29%) at twelve months for EM. Adjusted intervention effect on sand fly density measured by incidence rate ratio ranged from 0.28 (0.23–0.34) at one month to 0.62 (0.51–0.75) at 12 months for DWL; 0.72 (0.62–0.85) at one month to 1.02 (0.86–1.22) at 12 months for ITN; and 0.89 (0.76–1.03) at one months to 1.49 (1.26–1.74) at 12 months for EM. Household acceptance of EM was 74% compared to 94% for both DWL and ITN. Operational cost per household in USD was about 5, 8, and 2 for EM, DWL and ITN, respectively. Minimal adverse reactions were reported for EM and ITN while 36% of households with DWL reported transient itching.InterpretationDWL is the most effective, durable and acceptable control method followed by ITN. The Visceral Leishmaniasis (VL) Elimination Program in the Indian sub-continent should consider DWL and ITN for sand fly control in addition to IRS. 相似文献
43.
Ankit Kumar Yadav Suman Manna K. Pandiyan Arjun Singh Murugan Kumar Hillol Chakdar Prem Lal Kashyap Alok Kumar Srivastava 《Microbiology》2016,85(1):56-62
Among hydrocarbon pollutants, diesel oil is a complex mixture of alkanes and aromatic compounds which are often encountered as soil contaminants leaking from storage tanks and pipelines or as result of accidental spillage. One of the best ecofriendly approaches is to restore contaminated soil by using microorganisms able to degrade those toxic compounds in a bioremediation process. In the present study, nineteen bacteria were isolated by enrichment culture technique from diesel spilled soil collected from electric generator shed of NBAIM, Mau. All the isolates were subjected to screening for lipase production and twelve isolates were found to be positive for lipase. When the isolates were screened for biosurfactant production using CTAB-methylene blue agar plates, only one isolate viz. 2NBDSH3 was found positive which was found to be phylogenetically closely related with Bacillus flexus. Despite having low emulsification index, the bacterium could degrade 88.6% of diesel oil in soil. Biosurfactant from the isolate was extracted and characterized through infra-red spectroscopy which indicated its possible lipopeptide nature which was further supported by strong absorption in UV range in the UV-Vis spectrum. The results of the present study indicated that the isolate either does not produce any bioemulsifier or produces very low amount of emulsifier rather it produces a lipopeptide biosurfactant which helps in degradation of diesel oil by lowering the surface tension. The bacterium thus isolated and characterized can serve as a promising solution for ecofriendly remediation of bacterium diesel contaminated soils. 相似文献
44.
45.
46.
Tanya Biswas Alok Kalra A. K. Mathur R. K. Lal Manju Singh Archana Mathur 《Applied microbiology and biotechnology》2016,100(11):4909-4922
47.
Lal B 《Journal of molecular modeling》2007,13(4):531-536
The mechanism of the enantioselective binding of L-histidine with C60 fullerene and its derivatives, (1,2-methanofullerene C60)-61-carboxylic acid, diethyl (1,2-methanofullerene C60)-61-61-dicarboxylate and tert-butyl (1,2-methanofullerene C60)-61-carboxylate based chiral selectors was studied by quantum chemical calculations. All the molecules were fully optimized at RHF/6-31G* basis set. Relative energies between the different complexes were subsequently estimated with single-point electronic energies computed using Møller-Plesset perturbation theory (MP2). Stability and feasibility of all the generated structures were supported by their respective energy minima and fundamental frequencies. It was observed that interaction of fullerene derivatives with L-histidine is due to the existence of hydrogen bonding forces during the complex formation. The intermolecular forces, flow of atomic charges, binding energy, hardness, dipole moment and localization of electrostatic potential are in agreement with enantioselective interaction of L-histidine with C60 fullerene and its derivatives. It is found that theoretical evaluation to be consistent with the experimental data. 相似文献
48.
Guillaume Fouché Thomas Michel Anaïs Lalève Nick X. Wang David H. Young Brigitte Meunier Danièle Debieu Sabine Fillinger Anne-Sophie Walker 《Environmental microbiology》2022,24(3):1117-1132
Acquired resistance is a threat to antifungal efficacy in medicine and agriculture. The diversity of possible resistance mechanisms and highly adaptive traits of pathogens make it difficult to predict evolutionary outcomes of treatments. We used directed evolution as an approach to assess the resistance risk to the new fungicide fenpicoxamid in the wheat pathogenic fungus Zymoseptoria tritici. Fenpicoxamid inhibits complex III of the respiratory chain at the ubiquinone reduction site (Qi site) of the mitochondrially encoded cytochrome b, a different site than the widely used strobilurins which inhibit the same complex at the ubiquinol oxidation site (Qo site). We identified the G37V change within the cytochrome b Qi site as the most likely resistance mechanism to be selected in Z. tritici. This change triggered high fenpicoxamid resistance and halved the enzymatic activity of cytochrome b, despite no significant penalty for in vitro growth. We identified negative cross-resistance between isolates harbouring G37V or G143A, a Qo site change previously selected by strobilurins. Double mutants were less resistant to both QiIs and quinone outside inhibitors compared to single mutants. This work is a proof of concept that experimental evolution can be used to predict adaptation to fungicides and provides new perspectives for the management of QiIs. 相似文献
49.
50.
Jayakumar T. T. K. Shakhovskoy I. B. Prasoon N. P. K. Kathirvelpandian A. Ajith Kumar T. T. Lal K. K. 《Journal of Ichthyology》2019,59(5):697-706
Journal of Ichthyology - The present study reports the first record of the rearfin flying fish, Cypselurus opisthopus (Bleeker, 1865), in the south-eastern Arabian Sea based on a single specimen... 相似文献