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31.
Christian Biémont Cristina Vieira Christine Hoogland Géraldine Cizeron Catherine Lœvenbruck Claude Arnault Jean-Pierre Carante 《Genetica》1997,100(1-3):161-166
To investigate the main forces controlling the containment of transposable elements (TE) in natural populations, we analyzed
the copia, mdg1, and 412 elements in various populations of Drosophila melanogaster and D. simulans. A lower proportion of
insertion sites on the X chromosome in comparison with the autosomes suggests that selection against the detrimental effects
of TE insertions is the major force containing TE copies in populations of Drosophila. This selection effect hypothesis is
strengthened by the absence of the negative correlation between recombination rate and TE copy number along the chromosomes,
which was expected under the alternative ectopic exchange model (selection against the deleterious rearrangements promoted
by recombination between TE insertions). A cline in 412 copy number in relation to latitude was observed among the natural
populations of D. simulans, with very high numbers existing in some local populations (around 60 copies in a sample from Canberra,
Australia). An apparent absence of selection effects in this Canberra sample and a value of transposition rate equal to 1–2
× 10-3 whatever the population and its copy number agree with the idea of recent but temporarily drastic TE movements in local populations.
The high values of transposition rate in D. simulans clearly disfavor the hypothesis that the low amount of transposable elements
in this species could result from a low transposition rate.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
32.
33.
Dr J. O. Lopes D. V. Pereira L. A. Streher A. A. Fenalte S. H. Alves J. P. Benevenga 《Mycopathologia》1995,130(2):89-92
A case of cutaneous zygomycosis caused byAbsidia coryabifera in a leukemic patient submitted to chemotherapy is reported. The lesion was located on the little finger of the right hand and probably resulted from a latent osteomyelitis. It progressed to form extensive necrotic area. No systemic infection was detected and the lesion did not appear to be associated with any trauma. 相似文献
34.
Bernadette M. Kelly-Basetti Darren J. Cundy Suzanne M. Pereira Wolfgang H. F. Sasse G. Paul Savage Gregory W. Simpson 《Bioorganic & medicinal chemistry letters》1995,5(24):2989-2992
A series of substituted 2,2′-bipyridine derivatives was prepared using the Kröhnke reaction and alkylation of 4,4′-dimethyl-2,2′-bipyridine. These compounds were screened for fungicidal activity against 9 plant diseases. 5-Phenyl-2,2′-bipyridine exhibited strong preventative and curative fungicidal activity against wheat powdery mildew (Erisyphe graminis) and wheat leaf rust (Puccinia recondita). 相似文献
35.
M P Deane R H Mangia N M Pereira H Momen A M Gon?alves C M Morel 《Memórias do Instituto Oswaldo Cruz》1984,79(4):495-497
From an initial double infection in mice, established by simultaneous and equivalent inocula of bloodstream forms of strains Y and F of Trypanosoma cruzi, two lines were derived by subinoculations: one (W) passaged every week, the other (M) every month. Through biological and biochemical methods only the Y strain was identified at the end of the 10th and 16th passages of line W and only the F strain at the 2nd and 4th passages of line M. The results illustrate strain selection through laboratory manipulation of initially mixed populations of T. cruzi. 相似文献
36.
37.
Summary When the Na electrochemical potential difference across the skin (Na) is altered by perturbing the transmembrane electrical potential difference or the external Na concentration, effects on transport and associated oxygen consumption can be described by the formalism of linear nonequilibrium thermodynamics (Vieira, Caplan & Essig, 1972,J. Gen. Physiol.
59:77; Danisi & Lacaz-Vieira, 1974,J. Gen. Physiol.
64:372; Procópio and Lacaz-Vieira, 1977,J. Membrane Biol.
35:219). We now show that with modifications of Na by substitution of Li or choline for Na in the inner bathing solution, this formalism is no longer applicable. Inner Na by K substitution ((Na×K)
i
) causes profound alterations in short-circuit current (SCC),J
Na
in
, K efflux (J
K
eff
) and PD. SCC drops transiently after (Na×K)
i
in Cl and in SO4 media, increasing subsequently. In Cl medium, following the initial transient, there is a late decline in SCC toward a steady state. The rate of SCC decline in Cl medium is more pronounced than that observed in SO4 medium. (Na×K)
i
causes a transient increase inJ
Na
in
with a peak synchronous to the minimum in SCC, both in Cl and in SO4 media. This was interpreted as due to depolarization of the inner membrane. In SO4 medium, following the peak observed after (Na×K)
i
J
Na
in
drops, to increase again toward a steady state in which SCC andJ
Na
in
are not statistically different, resembling the control condition before (Na×K)
i
. In Cl medium, however, theJ
Na
in
steady state is approximately 100% higher than SCC. This difference is due to an important K efflux (J
K
eff
), which builds up progressively after the substitution. The apparent K permeability [J
K
eff
/(K
i
)] is of comparable magnitude in Cl and in SO4 media before (Na×K)
i
, the apparent K permeability increases one order of magnitude as compared to the control condition before the ionic substitution. In Cl medium, the high levels ofJ
Na
in
and ofJ
K
eff
observed in the steady state after (Na×K)
i
were interpreted as being a consequence of cell swelling. SCC and PD follow very different temporal patterns after (Na×K)
i
which are characterized by transients in SCC and a simple fall in PD. Reasons for these differences are discussed. 相似文献
38.
Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods 总被引:8,自引:4,他引:4 下载免费PDF全文
Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells. 相似文献
39.
Immunocytolocalization of glutamine synthetase in mesophyll and phloem of leaves ofSolanum tuberosum L. 总被引:1,自引:0,他引:1
Summary Localization of glutamine synthetase inSolanum tuberosum leaves was investigated by techniques of Western tissue printing and immunogold electron microscopy. Anti-GS antibodies used in immunolocalization recognize two peptides (45 kDa and 42 kDa) on Western blots. Antibody stained tissue prints on nitrocellulose membranes allowed low resolution localization of GS. Immunostaining was most evident in the adaxial phloem of the leaf midribs and petiole veins. High-resolution localization of glutamine synthetase by immunogold electron microscopy revealed that this enzyme occurs in both the chloroplasts and the cytosol ofS. tuberosum leaf cells. However, GS was specifically associated with the chloroplasts of mesophyll cells and with the cytoplasm of phloem companion cells. The evidence for cell-specific localization of chloroplast and cytosolic GS presented here agrees with the recently reported cell-specific pattern of expression of GUS reporter gene, directed by promoters for chloroplast and cytosolic GS form in tobacco transgenic plants. These data provide additional clues to the interpretation of the functional role of these different isoenzymes and its relationship with their specific localization.Abbreviations BSA
bovine serum albumin
- EM
electron microscope
- GOGAT
glutamate synthase
- GS
glutamine synthetase
- GUS
-glucuronidase
- IgG
immunoglobulin
- PBS
phosphate buffer saline
- SDS-PAGE
sodium dodecyl sulphate-polyacrylamide gel electrophoresis 相似文献
40.
H. Mercier C. C. J. Vieira R. C. L. Figueiredo-Ribeiro 《Plant Cell, Tissue and Organ Culture》1992,28(3):249-254
Leaf and stem segments of Gomphrena officinalis originated from aseptically grown seedlings were used to initiate cultures. Callus production was obtained on gelled Murashige & Skoog medium supplemented with 6-benzylaminopurine alone (1.0, 5.0 or 10.0 mgl-1) or combined with -naphthalene acetic acid (0.1, 0.5 and 1.0 mgl-1) after 10 to 15 days of culture, and can be transferred to fresh medium every 30 days. The combinations of 5.0 or 10.0 mgl-1 of 6-benzylaminopurine with 0.1 mgl-1 of -naphthalene acetic acid were found to be the best for shoot regeneration. Adventitious shoot formation occurred after 50 to 60 days of culture in leaf and internode stem explants. Nodal segments developed actively growing lateral buds after 30 days of culture. Gelled Murashige & Skoog medium containing 10 mgl-1 of indole-3-butyric acid was considered optimal for the rooting of shoots. Rooted plants transferred to potting soil could be successfully established.Abbreviations BA
6-benzylaminopurine
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige & Skoog
- NAA
-naphthalene acetic acid 相似文献