Bacterial leaching of manganese ores

Leaching of various types of ores, containing 12–30% manganese, by the thiobacterium Acidithiobacillus ferrooxidans was studied. Leaching of reduced ores (manganocalcite and manganiferous limestone) was mediated mainly by degradation of manganiferous minerals (by sulfuric acid produced in the course of bacterial oxidation of pyrite or sulfur). Bacterial treatment of the ores for 144 and 192 h allowed solubilization of 96–98% of manganese. Inoculation of bacteria into pulp with pyrite increased the rate of leaching of oxide ore (psilomelane) by 37%, and the degree of its extraction within 180 h increased from 80 to 97%.     

Mutation Analysis of IDH1 in Paired Gliomas Revealed IDH1 Mutation Was Not Associated with Malignant Progression but Predicted Longer Survival

Recurrence and progression to higher grade lesions are characteristic behaviorsof gliomas. Though IDH1 mutation frequently occurs and is considered as an early event in gliomagenesis, little is known about its role in the recurrence and progression of gliomas. We therefore analysed IDH1 and IDH2 statusat codon 132 of IDH1 and codon 172 of IDH2 by direct sequencing and anti-IDH1-R132H immunohistochemistry in 53 paired samples and their recurrences, including 29 low- grade gliomas, 16 anaplastic gliomas and 8 Glioblastomas. IDH1/IDH2 mutation was detected in 32 primarytumors, with 25 low- grade gliomas and 6 anaplastic gliomas harboring IDH1 mutation and 1 low- grade glioma harboring IDH2 mutation. All of the paired tumors showed consistent IDH1 and IDH2 status. Patients were analyzed according to IDH1 status and tumor-related factors. Malignant progression at recurrence was noted in 22 gliomas and was not associated with IDH1 mutation. Survival analysis revealed patients with IDH1 mutated gliomas had a significantly longer progression-free survival (PFS) and overall survival (OS). In conclusion, this study demonstrated a strong tendency of IDH1/IDH2 status being consistent during progression of glioma. IDH1 mutation was not a predictive marker for malignant progression and it was a potential prognostic marker for gliomas of Chinese patients.     

Orem自理模式在TURBT术后自我护理中的应用

目的：探讨Orem自理模式在经尿道膀胱肿瘤电切患者护理中的应用,以Orem自护理论作为指导,对膀胱肿瘤患者电切术后实行护理干预,观察Orem自理模式对膀胱肿瘤电切术后患者生活质量及术后复发率的影响。方法：选择2008年10月至2012年6月我科经尿道膀胱肿瘤电切术患者160例随机分为两组,即对照组和实验组,各80例。其中对照组采用术后常规护理,实验组将Orem自理模式贯穿于常规护理的全过程。比较两组患者术后下床活动时间、住院时间、患者对相关知识的掌握情况及患者满意度。结果：实验组患者术后下床活动时间、住院时间、患者对疾病的认知度、患者满意度均明显高于对照组（P〈0．05）。结论iOrem自理模式应用于经尿道膀胱肿瘤电切术后患者的护理,训练患者及家属自我护理技术和能力,不仅能调动和激发经尿道膀胱肿瘤电切患者主观能动性,变“替代护理”为“自我护理”,而且能减少并发症的发生,提高患者的自理能力及生存质量,初步实现了对经尿道膀胱肿瘤电切患者教育的个体化、系统性、连贯性和重复性。     

原儿茶酸对帕金森模型鼠脑组织抗氧化能力的影响

目的:观察原儿茶酸对鱼藤酮诱导的帕金森模型小鼠脑组织中相关抗氧化酶活性的影响,为原儿茶酸治疗帕金森病提供理论依据。方法:选用昆明小鼠为实验动物,连续5周腹腔注射鱼藤酮(1mg/kg.d)建立帕金森模型,之后2周分别注射原儿茶酸(5mg/kg.d)和阳性药物美多芭(125mg/kg.d)。应用生物化学方法,观察原儿茶酸对帕金森模型小鼠中脑和纹状体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力以及脂质过氧化物丙二醛(MDA)含量的影响。结果:实验数据显示原儿茶酸可提高帕金森模型小鼠中脑和纹状体SOD、CAT、GSH-PX活力,降低MDA含量。同时病理切片染色显示原儿茶酸可减轻鱼藤酮诱导的脑组织损伤。结论:原儿茶酸具有神经保护作用,保护机制可能是提高脑组织内源性抗氧化酶活力,减少体内自由基的产生,进而减轻了脑组织的病理损伤。     

Application of Spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas

To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.     

Discharge characteristics and dynamics of compressive plasma streams generated by a compact magnetoplasma compressor

Results from experimental studies of a compact magnetoplasma compressor designed for operation with heavy gases are presented. The integral characteristics of the discharge and the energy contents and other parameters of the generated xenon plasma streams are determined.     

Synaptic interrelationships between the segments of the heteromorphic bivalent in double heterozygotes for paracentric inversions in chromosome 1 of the house mouse

Electron microscopic analysis of synaptonemal complexes in bouble heterozygotes for the partially overlapping inversions In(1) 1Rk and In(1)12Rk in chromosome 1 of the house mouse was carried out. A great variety of synaptic configurations with complicated combinations of homologously and non-homologously paired segments was observed. Analysis of these configurations revealed at least five independent pairing regions in chromosome 1. Interrelationships between these regions with respect to their pairing ability were estimated. Pairings in the distal non-inverted segment and in inversions inhibit each other, while pairing in either inverted segment facilitates synapsis in the other. In other words, pairing initiations in different parts of the same bivalent are not independent events.by H.C. MacgregorDedicated to Dr. Ann Chandley in view of her important contributions to the study of meiosis     

Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags

The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty‐one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers.