排序方式: 共有83条查询结果,搜索用时 0 毫秒
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Fan Zhang Maitree Biswas Shabnam Massah Joseph Lee Shreyas Lingadahalli Samantha Wong Christopher Wells Jane Foo Nabeel Khan Helene Morin Neetu Saxena Sonia
H Y Kung Bei Sun Ana
Karla Parra
Nuez Christophe Sanchez Novia Chan Lauren Ung Umut
Berkay Altnta Jennifer
M Bui Yuzhuo Wang Ladan Fazli Htoo
Zarni Oo Paul
S Rennie Nathan
A Lack Artem Cherkasov Martin
E Gleave Jrg Gsponer Nada Lallous 《Nucleic acids research》2023,51(1):99
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Thomas Ibanez Cláudia Baider Chris Birkinshaw Heike Culmsee Susan Cordell F. B. Vincent Florens Janet Franklin Christian P. Giardina Thomas W. Gillespie Melinda Laidlaw Creighton M. Litton Tara G. Martin Rebecca Ostertag Narayanaswamy Parthasarathy Richard Randrianaivo Miramasoandro Randrianjanahary Muthu Rajkumar Ladan Rasingam Fidy Ratovoson Ludovic Reza Lawren Sack Shin‐ichiro Aiba Edward Webb Timothy J. S. Whitfeld Runguo Zang Philippe Birnbaum 《Global Ecology and Biogeography》2018,27(4):474-486
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Ladan Mansouri Josefin M. Paulsson Ali Moshfegh Stefan H. Jacobson Joachim Lundahl 《PloS one》2013,8(8)
Introduction
In Chronic Kidney Disease (CKD), immune cells are affected by uremic retention toxins. Given this effect, we analyzed lymphocyte proliferative response and immune modulators production following in vitro stimulation.Methods
Whole blood was drawn from healthy controls, patients with eGFR <20 ml/min/1.73 m2 (Pre-dialysis, CKD stages 4 and 5) and hemodialysis patients (stage 5D). Peripheral cells were incubated for six days with pokeweed mitogen, concanavalin A, Staphylococcus enterotoxin A or influenza A vaccine. Peripheral lymphocyte proliferation was then analyzed by the “Flow-cytometric Assay of Specific Cell-mediated Immune response in Activated whole blood” (FASCIA) method, and cytokine profile in the cell supernatants was analyzed by the Milliplex multi-array method.Results
The absolute number of lymphoblasts in response to mitogenic stimulation and the number of cells in each CD4+ and CD8+ subpopulation were similar comparing the three groups, except for a single decline in number of lymphoblasts after stimulation with Staphylococcus enterotoxin A, comparing dialysis patients with healthy controls. Levels of interleukin (IL)-2 (p=0.026), -10 (p=0.019) and -15 (p=0.027) in the Staphylococcus enterotoxin A-stimulated supernatant were lower in hemodialysis patients compared to healthy controls. Levels of IL-15 (p=0.017) from pre-dialysis patients and levels of IL-5 (p=0.019) from hemodialysis patients in influenza A vaccine-stimulated supernatants were also lower compared to controls. In pokeweed mitogen–stimulated supernatant, IL-2 levels (p=0.013) were lower in hemodialysis patients compared to pre-dialysis patients. TNF-α, IL-10, IL-12, IL-15, IL-8, MCP-1, IP-10, IFN-α2, IL-1α and eotaxin levels were all significantly higher in plasma obtained from CKD patients.Conclusion
Our results suggest that T-cells from CKD patients have similar proliferative response to stimulation compared with healthy individuals. Moreover, however the immune cells show inability to produce selected cytokines, most likely due to the uremic milieu or dialysis procedure. 相似文献79.
Mohammad Hojjat-Farsangi Abdul Salam Khan Amir Hossein Daneshmanesh Ali Moshfegh ?sa Sandin Ladan Mansouri Marzia Palma Jeanette Lundin Anders ?sterborg H?kan Mellstedt 《PloS one》2013,8(10)
Phosphorylation of receptor tyrosine kinases (RTKs) has a key role in cellular functions contributing to the malignant phenotype of tumor cells. We and others have previously demonstrated that RTK ROR1 is overexpressed in chronic lymphocytic leukemia (CLL). Silencing siRNA downregulated ROR1 and induced apoptosis of CLL cells. In the present study we analysed ROR1 isoforms and the phosphorylation pattern in CLL cells (n=38) applying western blot and flow-cytometry using anti-ROR1 antibodies and an anti-phospho-ROR1 antibody against the TK domain. Two major ROR1 bands with the size of 105 and 130 kDa respectively were identified, presumably representing unglycosylated (immature) and glycosylated (mature) ROR1 respectively as well as a 260 kDa band which may represent dimerized ROR1. A ROR1 band of 64 kDa that may correspond to a C-terminal fragment was also noted, present only in the nucleus. The 105 kDa ROR1 isoform was more frequently expressed in non-progressive as compared to progressive CLL patients (p=0.03). The 64, 105, 130 and 260 kDa bands were constitutively phosphorylated both at tyrosine and serine residues. Phosphorylation intensity of the mature (130 kDa) isoform was significantly higher in progressive than in non-progressive disease (p<0.001). Incubation of CLL cells with a mouse anti-ROR1 KNG or an anti-ROR1 CRD mAb respectively induced dephosphorylation of ROR1 before entering apoptosis. In conclusion CLL cells expressed different isoforms of ROR1 which were constitutively phosphorylated. The mature, phosphorylated ROR1 isoform was associated with a progressive disease stage. Targeting ROR1 by mAbs induced specific dephosphorylation and leukemic cell death. ROR1 might be an interesting therapeutic target. 相似文献
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