全文获取类型
收费全文 | 274篇 |
免费 | 14篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 20篇 |
2020年 | 4篇 |
2019年 | 8篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 17篇 |
2015年 | 19篇 |
2014年 | 24篇 |
2013年 | 25篇 |
2012年 | 25篇 |
2011年 | 25篇 |
2010年 | 10篇 |
2009年 | 10篇 |
2008年 | 13篇 |
2007年 | 10篇 |
2006年 | 10篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 5篇 |
2001年 | 5篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1975年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有288条查询结果,搜索用时 187 毫秒
71.
Thomaz Pinotti Anders Bergström Maria Geppert Matt Bawn Dominique Ohasi Wentao Shi Daniela R. Lacerda Arne Solli Jakob Norstedt Kate Reed Kim Dawtry Fabricio González-Andrade Cesar Paz-y-Miño Susana Revollo Cinthia Cuellar Marilza S. Jota José E. Santos Qasim Ayub Chris Tyler-Smith 《Current biology : CB》2019,29(1):149-157.e3
72.
Zanuncio JC Lemos WP Lacerda MC Zanuncio TV Serrão JE Bauce E 《Journal of economic entomology》2006,99(2):401-407
Reproductive potential, longevity, life expectancy, and fertility life tables of Brontocoris tabidus (Signoret) (Heteroptera: Pentatomidae), a predator of lepidopteran defoliators in eucalyptus (Eucalyptus spp.) plantations, were studied in the field. After a 50-d preoviposition period (emergence of adults to the deposition of the first egg mass), ovipositional activity of B. tabidus continued until females died at 160 d. Females laid an average of 4.2 eggs per day and 601.1 eggs in a lifetime. Gross and net reproductive rates were 216.7 and 75.8 females, respectively. Generation time was 146.1 d, the period for doubling the population was 23.4 d, intrinsic rate was 0.03, and finite population increase was 1.03. Number of females per generation increased at 33.4 times. Results from our field studies indicate that B. tabidus has greater potential reproduction, oviposition period, and longevity than was expected from previous laboratory experiments. This suggests that B. tabidus has potential as a biological control agent to limit economically damaging pests in eucalyptus plantations. 相似文献
73.
Juan Carlos Alvarez-Pizarro Enéas Gomes-Filho Claudivan Feitosa de Lacerda Nara Lídia Mendes Alencar José Tarquínio Prisco 《Plant Growth Regulation》2009,59(2):125-135
The effects of NaCl stress on the growth, water relation, gas exchange, tissue mineral content, and on H+-ATPase activity, lipid composition and peroxidation of root plasma membrane-enriched fractions of two genotypes (CCP06 and
BRS189, sensitive and tolerant to salt stress, respectively) of dwarf-precocious cashew were studied. Growth reduction was
higher in CCP06 than in BRS189. Net photosynthesis decreased in both genotypes, CCP06 being more affected. Roots of BRS189
accumulated higher amount of Na+ than those of CCP06 at both salt treatments, whereas Cl− increase was higher only at 8 dS m−1. NaCl at 8 dS m−1 did not modify the plasma membrane H+-ATPase activity in CCP06 roots, but significantly increased it in BRS189 roots. Lipid peroxidation was lower in BRS189 than
in CCP06 roots. Salinity induced higher accumulation of proline in BRS189 roots. Total phospholipids and free sterols content
increased significantly in root plasma membrane of CCP06. However, in BRS189, a slight reduction of free sterols content and
no changes in total phospholipids content were observed. Thus, the results suggest that the ability of cashew seedlings to
adapt to salt stress is, at least in part, dependent upon the maintenance of integrity and protection against oxidative damage
of plasma membrane, which could favor the activation of plasma membrane H+-ATPase, as a cellular mechanism to regulate ion exclusion from the shoot. 相似文献
74.
Thaís Leal-Silva Flaviane Vieira-Santos Fabrício Marcus Silva Oliveira Luiza de Lima Silva Padro Lucas Kraemer Pablo Hemanoel da Paixo Matias Camila de Almeida Lopes Ana Cristina Loiola Ruas Isabella Carvalho de Azevedo Denise Silva Nogueira Milene Alvarenga Rachid Marcelo Vidigal Caliari Remo Castro Russo Ricardo Toshio Fujiwara Lilian Lacerda Bueno 《PLoS neglected tropical diseases》2021,15(7)
Toxocariasis is a neglected disease that affects people around the world. Humans become infected by accidental ingestion of eggs containing Toxocara canis infective larvae, which upon reaching the intestine, hatch, penetrate the mucosa and migrate to various tissues such as liver, lungs and brain. Studies have indicated that Th2 response is the main immune defense mechanism against toxocariasis, however, there are still few studies related to this response, mainly the IL-33/ST2 pathway. Some studies have reported an increase in IL-33 during helminth infections, including T. canis. By binding to its ST2 receptor, IL-33 stimulating the Th2 polarized immune cell and cytokine responses. Thus, we aimed to investigate the role of the IL-33/ST2 pathway in the context of T. canis larval migration and the immunological and pathophysiological aspects of the infection in the liver, lungs and brain from Wild-Type (WT) BALB/c background and genetically deficient mice for the ST2 receptor (ST2-/-). The most important findings revealed that the IL-33/ST2 pathway is involved in eosinophilia, hepatic and cerebral parasitic burden, and induces the formation of granulomas related to tissue damage and pulmonary dysfunction. However, ST2-/- mice, the immune response was skewed to Th1/Th17 type than Th2, that enhanced the control of parasite burden related to IgG2a levels, tissue macrophages infiltration and reduced lung dysfunction. Collectively, our results demonstrate that the Th2 immune response triggered by IL-33/ST2 pathway mediates susceptibility to T. canis, related to parasitic burden, eosinophilia and granuloma formation in which consequently contributes to tissue inflammation and injury. 相似文献
75.
Stephanie Zobrist Marcelo Brito Eduardo Garbin Wuelton M. Monteiro Suellen Clementino Freitas Marcela Macedo Aline Soares Moura Nicole Advani Maria Kahn Sampa Pal Emily Gerth-Guyette Pooja Bansil Gonzalo J. Domingo Dhelio Pereira Marcus VG Lacerda 《PLoS neglected tropical diseases》2021,15(8)
BackgroundGlucose-6-phosphate dehydrogenase (G6PD) deficiency is a common enzyme deficiency, prevalent in many malaria-endemic countries. G6PD-deficient individuals are susceptible to hemolysis during oxidative stress, which can occur from exposure to certain medications, including 8-aminoquinolines used to treat Plasmodium vivax malaria. Accordingly, access to point-of-care (POC) G6PD testing in Brazil is critical for safe treatment of P. vivax malaria.Methodology/Principal findingsThis study evaluated the performance of the semi-quantitative, POC STANDARD G6PD Test (SD Biosensor, Republic of Korea). Participants were recruited at clinics and through an enriched sample in Manaus and Porto Velho, Brazil. G6PD and hemoglobin measurements were obtained from capillary samples at the POC using the STANDARD and HemoCue 201+ (HemoCue AB, Sweden) tests. A thick blood slide was prepared for malaria microscopy. At the laboratories, the STANDARD and HemoCue tests were repeated on venous samples and a quantitative spectrophotometric G6PD reference assay was performed (Pointe Scientific, Canton, MI). G6PD was also assessed by fluorescent spot test. In Manaus, a complete blood count was performed.Samples were analyzed from 1,736 participants. In comparison to spectrophotometry, the STANDARD G6PD Test performed equivalently in determining G6PD status in venous and capillary specimens under varied operating temperatures. Using the manufacturer-recommended reference value thresholds, the test’s sensitivity at the <30% threshold on both specimen types was 100% (95% confidence interval [CI] venous 93.6%–100.0%; capillary 93.8%–100.0%). Specificity was 98.6% on venous specimens (95% CI 97.9%–99.1%) and 97.8% on capillary (95% CI 97.0%–98.5%). At the 70% threshold, the test’s sensitivity was 96.9% on venous specimens (95% CI 83.8%–99.9%) and 94.3% on capillary (95% CI 80.8%–99.3%). Specificity was 96.5% (95% CI 95.0%–97.6%) and 92.3% (95% CI 90.3%–94.0%) on venous and capillary specimens, respectively.Conclusion/SignificanceThe STANDARD G6PD Test is a promising tool to aid in POC detection of G6PD deficiency in Brazil.Trial registrationThis study was registered with ClinicalTrials.gov (identifier: ). NCT04033640相似文献
76.
Samanta Oliveira Loureiro Luana Heimfarth Bruna Arcce Lacerda Luiza Fedatto Vidal Angela Soska Natália Gomes dos Santos Angela Terezinha de Souza Wyse Regina Pessoa-Pureur 《Cellular and molecular neurobiology》2010,30(4):557-568
In this study, we investigated the actions of high homocysteine (Hcy) levels (100 and 500 μM) on the cytoskeleton of C6 glioma
cells. Results showed that the predominant cytoskeletal response was massive formation of actin-containing filopodia at the
cell surface that could be related with Cdc42 activation and increased vinculin immunocontent. In cells treated with 100 μM
Hcy, folic acid, trolox, and ascorbic acid, totally prevented filopodia formation, while filopodia induced by 500 μM Hcy were
prevented by ascorbic acid and attenuated by folic acid and trolox. Moreover, competitive NMDA ionotropic antagonist DL-AP5
totally prevented the formation of filopodia in both 100 and 500 μM Hcy treated cells, while the metabotropic non-selective
group I/II antagonist MCPG prevented the effect of 100 μM Hcy but only slightly attenuated the effect induced by of 500 μM
Hcy on actin cytoskeleton. The competitive non-NMDA ionotropic antagonist CNQX was not able to prevent the effects of Hcy
on the reorganization of actin cytoskeleton in the two concentrations used. Also, Hcy-induced hypophosphorylation of vimentin
and glial fibrillary acidic protein (GFAP) and this effect was prevented by DL-AP5, MCPG, and CNQX. In conclusion, our results
show that Hcy target the cytoskeleton of C6 cells probably by excitoxicity and/or oxidative stress mechanisms. Therefore,
we could propose that the dynamic restructuring of the actin cytoskeleton of glial cells might contribute to the response
to the injury provoked by elevated Hcy levels in brain. 相似文献
77.
Removal of heavy metals by an Aspergillus terreus strain immobilized in a polyurethane matrix 总被引:1,自引:0,他引:1
Dias MA Lacerda IC Pimentel PF de Castro HF Rosa CA 《Letters in applied microbiology》2002,34(1):46-50
AIMS: The aim was to investigate the biosorption of chromium, nickel and iron from metallurgical effluents, produced by a steel foundry, using a strain of Aspergillus terreus immobilized in polyurethane foam. METHODS AND RESULTS: A. terreus UFMG-F01 was immobilized in polyurethane foam and subjected to biosorption tests with metallurgical effluents. Maximal metal uptake values of 164.5 mg g(-1) iron, 96.5 mg g(-1) chromium and 19.6 mg g(-1) nickel were attained in a culture medium containing 100% of effluent stream supplemented with 1% of glucose, after 6 d of incubation. CONCLUSIONS: Microbial populations in metal-polluted environments include fungi that have adapted to otherwise toxic concentrations of heavy metals and have become metal resistant. In this work, a strain of A. terreus was successfully used as a metal biosorbent for the treatment of metallurgical effluents. SIGNIFICANCE AND IMPACT OF THE STUDY: A. terreus UFMG-F01 was shown to have good biosorption properties with respect to heavy metals. The low cost and simplicity of this technique make its use ideal for the treatment of effluents from steel foundries. 相似文献
78.
J. R. De Oliveira R. Da Silva Romeiro B. De Souza Leäo Lacerda 《Journal of Phytopathology》1994,140(4):363-366
Occurrence of crown gall on grape was observed in a vineyard in Minas Gerais State, Brazil. Isolations of the pathogen yielded a bacterium which incited galls on grapevine, tomato and bryophillum. The bacterium was confirmed as Agrobacterium tumefaciens and its characterization showed it to be biovar 3. This is the first report on the occurrence of the biovar 3 of A. tumefaciens on grapevine in Brazil. 相似文献
79.
Wilson?MachadoEmail author Bruno B.?Gueiros Sebasti?o D.?Lisboa-Filho Luiz D.?Lacerda 《Wetlands Ecology and Management》2005,13(2):199-206
Metal-rich mineral deposits on the roots of aquatic plants, denominated iron plaques, may moderate the uptake of essential, but potentially toxic metals by roots. We investigated the iron plaque formation on the fine, nutritive roots of mangrove seedlings growing in contrasting environments (oxidizing sand flat sediments and reducing mangrove forest sediments) in southeast Brazil. The results indicate that Avicennia schaueriana, Laguncularia racemosa, and Rhizophora mangle seedlings developed an efficient exclusion of Fe, Mn, and Zn through iron plaque formation. This process seems to be influenced substantially by species-specific responses to environmental conditions. While Fe and Zn translocation to leaves appear to be suppressed by accumulation within root tissues, this did not appear to occur for Mn, suggesting that Mn trapping in rhizosphere sediments and iron plaque formation are the main mechanisms responsible for the Mn exclusion from the organism level. In addition to factors well recognized as affecting mangrove seedling development (e.g., salinity stress and nutrient availability), the mediation of trace metal uptake by iron plaque formation possibly contribute to determine the seedling adaptability to waterlogged conditions. 相似文献
80.
The proportions of males produced by queens or workers of Scaptotrigona depilis, and the sex-ratio in the brood, were estimated. Thirteen young combs were collected; from one half of each comb the cells
were opened and the number of eggs per cell was recorded. Later, upon maturation, from the other halves of the combs the individual
inside each cell was classified according to sex. If from a cell containing initially two eggs a male would emerge (supposedly
the son of a worker) the proportion of such cells in the comb would represent the maximum overall workers’ male production.
Consequently, the difference with the number of males found in the second half of the comb would indicate the minimum contribution
by the queen. In these 13 combs, on average, 7.3% of the cells contained 2 eggs, whilst in 30.9% of the cells a male developed.
Males were found in 10 combs, in 2–79% of the cells. In 6 of the 13 combs 2–47% of the cells contained 2 eggs. In these 6
combs more than 40% of the cells had a male inside. In 5 of them, the frequency of males was significantly higher than the
frequency of cells containing 2 eggs. Queens, therefore, produced the majority of the males. There was no correlation between
worker and queen numerical investments in male production, indicating different response mechanisms of workers, compared to
queens, to conditions favouring male production. There was also no correlation between the mean egg size of the queen and
the percentage males in the combs. 相似文献