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161.
卷果涩芥(Malcolmia scorpioides)、四齿芥(Tetracme quadricornis)、长齿四齿芥(T.recurvata)和狭果鹤虱(Lappula semiglabra)为新疆北部常见的四种短命植物。本文从种子生物学、发育节律、株形和植株寿命等方面研究它们与生存环境的协调与适应。 相似文献
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163.
研究了植物凤凰木种子中的蛋白质成分,试图分离出新的核糖体失活蛋白凤凰木种子经磷酸盐缓冲液抽提,硫酸铵分级盐析,分子筛和阴离子交换剂等多次柱层析,分离出三种组分DrⅠ、DrⅡ和DrⅢ.SDS-PAGE和IEF实验表明这三种蛋白质均达到单一纯,而HPLC实验则表明它们的纯度不低于90%。它们的分子量据SDS-PAGE和HPLC实验分别约23500、26000、28500.IEF实验测得三者的等电点均为5.2左右。测定了组分DrⅠ的蛙卵泡裂解活性,其毒性约为天花粉蛋白的1/40.分析了它们的氨基酸组成。估算了三种蛋白质的二级结构含量,结果表明三者均富含β折叠结构。已有的实验事实表明这三种蛋白质的生化行为明显异于核糖体失活蛋白,因而很可能不属于这一蛋白质家族。 相似文献
164.
Corticotropin-releasing hormone (CRH) has been shown to be a central mediator for most, if not all, stress-induced responses. Since stressful stimuli may decrease hypothalamic tuberoinfundibular and tuberohypophysial dopaminergic neuronal activities, we aimed to determine whether CRH is involved. Using central administration of various doses of ovine CRH (oCRH; 1, 3 and 10 µg/rat) into the lateral cerebroventricle of either male or female rats, the neurochemical changes in various parts of the central nervous system, including the hypothalamus, were determined by high-performance liquid chromatography at various times after the injection (30, 60, 120 and 240 min). The concentrations of 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxy-phenylethyleneglycol (MHPG), two major metabolites of dopamine and norepinephrine, respectively, in discrete brain regions were used as indices for catecholaminergic neuron activity. Plasma corticosterone levels increased significantly after all doses of oCRH and at all time points studied. oCRH also exerted significant stimulatory effects on noradrenergic neuron terminals in the frontal cortex, and on dopaminergic neuron terminals in the nucleus accumbens, hypothalamic paraventricular and periventricular nuclei, and intermediate pituitary lobe. Dopaminergic neuron terminals in the median eminence and the neural lobe of the pituitary, however, were not affected. There was no major difference in the responses between male and female rats. We conclude that CRH has a differential effect on central catecholaminergic neurons. 相似文献
165.
几种细胞分裂素诱导石竹外植体直接再分化成花芽的机理 总被引:4,自引:0,他引:4
用MS+2,4-D0.1mg/L为基本培养基,分别附加不同浓度的ZT、BA和KT,对石竹的叶片、茎和花等外植体直接再分化成花芽的试验表明:叶片的外植体在有BA时可再分化出只有红色花瓣的不正常花芽;在含ZT的培养基中再分化出具有1~2片叶或无叶的花芽,其花有两性花,雌花和仅单个雌蕊的不正常花,两性花可发育成结籽的果实;KT未能诱导花芽分化。茎外植体,3种CTK都不能诱导出花芽。花等外植体仅在KT3mg/L的培养中再分化出无叶的不正常花芽,ZT和BA的处理均不能诱导出花芽。 相似文献
166.
滇南及滇东南胶孔菌复合群的分类地理研究 总被引:1,自引:1,他引:0
报道了我国滇南及滇东南热带、亚热带胶孔菌复合群众路线属6种,其中瘤丝牛肝菌Filoboletus verruculosus P.G.Liu和滇丝牛肝菌F.yunnanensis P.G.Liu是新种,紫兰小菇Mycena violacella(Speq.)Sing。是我国新纪录种;新种附有拉丁文描术和插图,新纪录和附有形态解剖图。本文所引证标本均存放于中国科学院昆明植物研究所隐花植物标本馆(HKA 相似文献
167.
乌奴龙胆中五个新的环烯醚萜甙 总被引:4,自引:0,他引:4
从藏药乌奴龙胆(GentianaurnulaSmith)(龙胆科)的全草中分离到5个新的环烯醚萜甙,命名为乌奴龙胆甙(gentioumoside)A-E;它们的结构主要通过光谱分析得以确定。其中,乌奴龙胆A-C是二聚环烯醚萜甙,而乌奴龙胆甙D和E为马钱素型的环烯醚萜甙,所有这些化合物的分子中都具有一个2,3-二羟基苯甲酰基或其衍生物的取代基。 相似文献
168.
169.
The pulmonate limpets Siphonaria japonica and Siphonana siriusoccur over a wide range of local habitat types in terms of exposureto wave action and salinity. This is a study of these two specieson three different shore types in Hong Kong, ranging from anextremely exposed, high salinity (3235) shore at Caped'Aguilar to a sheltered, low salinity (1633) shore atTai Lam Chung. Both species are restricted to the low shore,year round. S. japonica is a winter breeder and recruitmentoccurred between October and January. The recruitment of S.sirius could not be recognised from the size-frequency histograms.The algal standing crop at Tai Lam Chung was higher than atWu Kwai Sha during the winter period, i.e., between Octoberand April. Seasonal fluctuations in growth rate were recordedfor both Siphonaria species with the time of fastest growthoccurring in winter. S. japonica grew faster at Tai Lam Chungthan at Wu Kwai Sha. Food availability is thought to be an importantfactor affecting growth. (Received 17 September 1993; accepted 16 June 1994) 相似文献
170.
A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells. 总被引:22,自引:5,他引:17
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J M Slingerland L Hengst C H Pan D Alexander M R Stampfer S I Reed 《Molecular and cellular biology》1994,14(6):3683-3694
Transforming growth factor beta (TGF-beta) is a potent inhibitor of epithelial cell growth. Cyclins E and A in association with Cdk2 have been shown to play a role in the G1-to-S phase transition in mammalian cells. We have studied the effects of TGF-beta-mediated growth arrest on G1/S cyclins E and A. Inhibition of cyclin A-associated kinase by TGF-beta is primarily due to a decrease in cyclin A mRNA and protein. By contrast, while TGF-beta inhibits accumulation of cyclin E mRNA, the reduction in cyclin E protein is minimal. Instead, we find that the activation of cyclin E-associated kinase that normally accompanies the G1-to-S phase transition is inhibited. A novel inhibitor of cyclin-Cdk complexes was detected in TGF-beta-treated cell lysates. Inhibition is mediated by a heat-stable protein that targets both Cdk2 and Cdc2 kinases. In G0-arrested cells, a similar inhibitor of Cdk2 kinase was detected. These data suggest the existence of an inhibitor of cyclin-dependent kinases induced under different conditions to mediate antiproliferative responses. 相似文献