全文获取类型
收费全文 | 128篇 |
免费 | 1篇 |
出版年
2017年 | 1篇 |
2014年 | 5篇 |
2013年 | 7篇 |
2012年 | 4篇 |
2011年 | 7篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 6篇 |
2007年 | 12篇 |
2006年 | 7篇 |
2005年 | 7篇 |
2004年 | 4篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2000年 | 1篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有129条查询结果,搜索用时 31 毫秒
31.
XUEQING HUANG JOHANNA SCHMITT LISA DORN CONVERSE GRIFFITH SIGI EFFGEN SHAUN TAKAO MAARTEN KOORNNEEF KATHLEEN DONOHUE 《Molecular ecology》2010,19(7):1335-1351
Colonizing species may often encounter strong selection during the initial stages of adaptation to novel environments. Such selection is particularly likely to act on traits expressed early in development since early survival is necessary for the expression of adaptive phenotypes later in life. Genetic studies of fitness under field conditions, however, seldom include the earliest developmental stages. Using a new set of recombinant inbred lines, we present a study of the genetic basis of fitness variation in Arabidopsis thaliana in which genotypes, environments, and geographic location were manipulated to study total lifetime fitness, beginning with the seed stage. Large‐effect quantitative trait loci (QTLs) for fitness changed allele frequency and closely approached 90% in some treatments within a single generation. These QTLs colocated with QTLs for germination phenology when seeds were dispersed following a schedule of a typical winter annual, and they were detected in two geographic locations at different latitudes. Epistatically interacting loci affected both fitness and germination in many cases. QTLs for field germination phenology colocated with known QTLs for primary dormancy induction as assessed in laboratory tests, including the candidate genes DOG1 and DOG6. Therefore fitness, germination phenology, and primary dormancy are genetically associated at the level of specific chromosomal regions and candidate loci. Genes associated with the ability to arrest development at early life stages and assess environmental conditions are thereby likely targets of intense natural selection early in the colonization process. 相似文献
32.
THE OBSTETRICS GYNECOLOGY RISK RESEARCH GROUP: REBECCA KUKLA MIRIAM KUPPERMANN MARGARET LITTLE ANNE DRAPKIN LYERLY LISA M. MITCHELL ELIZABETH M. ARMSTRONG LISA HARRIS 《Bioethics》2009,23(1):1-8
Over the last several years, as cesarean deliveries have grown increasingly common, there has been a great deal of public and professional interest in the phenomenon of women 'choosing' to deliver by cesarean section in the absence of any specific medical indication. The issue has sparked intense conversation, as it raises questions about the nature of autonomy in birth. Whereas mainstream bioethical discourse is used to associating autonomy with having a large array of choices, this conception of autonomy does not seem adequate to capture concerns and intuitions that have a strong grip outside this discourse. An empirical and conceptual exploration of how delivery decisions ought to be negotiated must be guided by a rich understanding of women's agency and its placement within a complicated set of cultural meanings and pressures surrounding birth. It is too early to be 'for' or 'against' women's access to cesarean delivery in the absence of traditional medical indications – and indeed, a simple pro- or con- position is never going to do justice to the subtlety of the issue. The right question is not whether women ought to be allowed to choose their delivery approach but, rather, taking the value of women's autonomy in decision-making around birth as a given, what sorts of guidelines, practices, and social conditions will best promote and protect women's full inclusion in a safe and positive birth process. 相似文献
33.
LISA M. SCHLOEGEL LUÍS FELIPE TOLEDO JOYCE E. LONGCORE SASHA E. GREENSPAN CONRADO AUGUSTO VIEIRA MARIA LEE SERENA ZHAO CATHERINE WANGEN CLAUDIA MARIS FERREIRA MÁRCIO HIPOLITO ANGELA J. DAVIES CHRISTINA A. CUOMO PETER DASZAK TIMOTHY Y. JAMES 《Molecular ecology》2012,21(21):5162-5177
Global amphibian declines are linked with the presence of specific, highly virulent genotypes of the emerging fungal disease chytridiomycosis caused by Batrachochytrium dendrobatidis (Bd) known as the global panzootic lineage (Bd‐GPL). The global trade in amphibians for human consumption is suspected to have facilitated emergence of the disease, but evidence to support this is largely lacking. Here, we investigated the role the Lithobates catesbeianus (North American bullfrog) trade in spreading Bd genotypes by comparing strains associated with L. catesbeianus to a global panel using 36 sequenced loci from multiple chromosomal regions. Most bullfrogs were infected with Bd‐GPL genotypes, but we also detected novel, highly divergent Bd genotypes (Bd‐Brazil) from a live bullfrog in a US market and from native Brazilian anurans in the Atlantic Forest where bullfrogs are widely farmed. Sexual reproduction was also detected for the first time in Bd in the form of a hybrid genotype between the Bd‐GPL and Bd‐Brazil lineages in the Atlantic Forest. Despite the demonstration that ribosomal RNA types in Bd fail to undergo concerted evolution (over 20 sequence types may be found in a single strain), the Bd‐GPL and Bd‐Brazil lineages form largely separate clusters of related internal transcribed spacer (ITS) RNA sequences. Using ITS sequences, we then demonstrate the presence of Bd‐Brazil in Japan, primarily on invasive L. catesbeianus. The finding that Bd is capable of sexual reproduction between panzootic and endemic genotypes emphasizes the risk of international wildlife trade as a source of additional Bd epizootics owing to hybridization. 相似文献
34.
1. Fish and ducks often belong to the same local food web, and several studies indicate that there is a general negative effect of fish on breeding ducks. This pattern has so far been addressed mainly within the framework of competition for common invertebrate prey, while predation by large fish as a force behind settlement and abundance patterns in ducks remains largely unknown. This is the first study to address the effect of fish predation on breeding ducks, isolated from that of competition, and the first experiment to explore the ability of ducks to identify and avoid lakes with high risk of fish predation. 2. We used a before–after control–impact design and 11 naturally fishless lakes. Waterfowl on the lakes were surveyed during the breeding season of 2005. Large adult pike (Esox lucius) were added to two lakes in early spring 2008, and waterfowl surveys were repeated on all 11 lakes. 3. Pike introduction did not affect the number of pairs on lakes during the nesting season in any of three focal duck species (mallard Anas platyrhynchos, teal Anas crecca, and goldeneye Bucephala clangula). During the brood‐rearing season, however, there was a decrease in duck days in teal and goldeneye in lakes with pike, with similar trends observed in mallard. The number of goldeneye ducklings was also significantly lower in lakes with pike. We were unable to determine whether the response was attributable to direct pike predation or to broods leaving experimental lakes, but in either case, our study demonstrates high fitness costs for ducks breeding on lakes with pike. 4. The apparent inability of nesting ducks to detect pike and the clear fitness implications may influence the annual recruitment of ducks on a larger scale as pike are both common and widespread. Vegetation complexity and food abundance are likely to be of overriding importance when breeding ducks are choosing a nesting site. As pike have a strong influence on breeding birds, relying on vegetation and cues of food abundance, while ignoring indicators of predation risk from fish, could lead to lakes with pike acting as an ecological trap. 相似文献
35.
36.
LISA AUCHINCLOSS HSIEN M. EASLON DIEDRE LEVINE LISA DONOVAN JAMES H. RICHARDS 《Plant, cell & environment》2014,37(6):1364-1370
Most C3 plant species have partially open stomata during the night especially in the 3–5 h before dawn. This pre‐dawn stomatal opening has been hypothesized to enhance early‐morning photosynthesis (A) by reducing diffusion limitations to CO2 at dawn. We tested this hypothesis in cultivated Helianthus annuus using whole‐shoot gas exchange, leaf level gas exchange and modelling approaches. One hour pre‐dawn low‐humidity treatments were used to reduce pre‐dawn stomatal conductance (g). At the whole‐shoot level, a difference of pre‐dawn g (0.40 versus 0.17 mol m?2 s?1) did not significantly affect A during the first hour after dawn. Shorter term effects were investigated with leaf level gas exchange measurements and a difference of pre‐dawn g (0.10 versus 0.04 mol m?2 s?1) affected g and A for only 5 min after dawn. The potential effects of a wider range of stomatal apertures were explored with an empirical model of the relationship between A and intercellular CO2 concentration during the half‐hour after dawn. Modelling results demonstrated that even extremely low pre‐dawn stomatal conductance values have only a minimal effect on early‐morning A for a few minutes after dawn. Thus, we found no evidence that pre‐dawn stomatal opening enhances A. 相似文献
37.
Crossosoma californicum (Crossosomataceae) is a rare shrub species endemic to the California Channel Islands. Previous studies based on allozymes revealed little genetic variability in this plant species. We have isolated 13 polymorphic microsatellite loci from C. californicum. These loci show intermediate levels of variability, averaging 4.2 alleles per locus and expected heterozygosity of 0.376. Two loci did not fit Hardy–Weinberg expectations with significant deficits of heterozygous genotypes consistent with the presence of null alleles or population subdivision. 相似文献
38.
Habitat conditions mediate the effects of climate, so neighboring populations with differing habitat conditions may differ in their responses to climate change. We have previously observed that juvenile survival in Snake River spring/summer Chinook salmon is strongly correlated with summer temperature in some populations and with fall streamflow in others. Here, we explore potential differential responses of the viability of four of these populations to changes in streamflow and temperature that might result from climate change. First, we linked predicted changes in air temperature and precipitation from several General Circulation Models to a local hydrological model to project streamflow and air temperature under two climate‐change scenarios. Then, we developed a stochastic, density‐dependent life‐cycle model with independent environmental effects in juvenile and ocean stages, and parameterized the model for each population. We found that mean abundance decreased 20–50% and the probability of quasi‐extinction increased dramatically (from 0.1–0.4 to 0.3–0.9) for all populations in both scenarios. Differences between populations were greater in the more moderate climate scenario than in the more extreme, hot/dry scenario. Model results were relatively robust to realistic uncertainty in freshwater survival parameters in all scenarios. Our results demonstrate that detailed population models can usefully incorporate climate‐change predictions, and that global warming poses a direct threat to freshwater stages in these fish, increasing their risk of extinction. Because differences in habitat may contribute to the individualistic population responses we observed, we infer that maintaining habitat diversity will help buffer some species from the impacts of climate change. 相似文献
39.
40.
SAMPLE/RINSE TEMPERATURE EFFECTS ON DISCRIMINATION OF SWEET AND SALTY DIFFERENCES IN A FOOD SYSTEM 总被引:1,自引:0,他引:1
The ability of panelists to distinguish between samples of apple juice with two levels of added sucrose (0.1 and 0.9 g/100 mL) or NaCl (0.05 and 0.14 g/100 mL) was determined using a modified signal detection technique. Samples at various temperatures (6, 24, or 50C) were presented with various rinse conditions. Sometimes a water rinse (6, 24, or 50C) was presented between samples and somtimes not. R-index values were calculated to predict the ability of panelists to distinguish between samples. No temperature effect was found for sweetness difference tests; however, R-indices showed that salty differences in apple juice were more distinguishable at a sample temperature of 24C than at 6 or 50C. Samples temperature had a greater effect than rinse condition (temperature or no rinse) on discrimination between samples differing in NaCl concentration. 相似文献