金翅夜蛾亚科Plusiinae的研究

金翅夜蛾亚科(Plusiinae)是夜蛾科中一个显著的类群,从眼的睫毛,M_2的存在与前翅通常具有金银斑可以认识。但是不同属、种在外形上区别极少,所以如果不根据生殖器,要正确鉴定所有的属种,几乎是不可能。 这一亚科全世界已记载的约230余种,其分布于古北区的有107种。中国已记载的共70种。陕西省已有记载的只有6种,可能分布到的约21种。1963年朱弘复、陈一心二同志在《中国经济昆虫志(第三册)鳞翅目夜蛾科(一)》一书中记述了11种,提到其中5     

金针菇HMG-box转录因子鉴定及一个候选基因的差异表达分析

食用菌的原基形成受到环境因子和内源基因的共同调控,转录因子在其生长发育过程中能够起到关键作用。本研究在已测序金针菇单核菌株L11全基因组中鉴定到29个高速泳动蛋白（high-mobility-group box,HMG-box）转录因子编码基因。基于金针菇双核菌株H1123各发育时期转录组数据,筛选到一个与金针菇原基形成相关的HMG-box转录因子基因Fv-hmg。通过同源比对发现Fv-hmg的DNA序列在6个不同金针菇菌株中十分保守,一致性为98.38%,有92个SNP位点。克隆分析表明,该基因长度为1 517bp,含有一个内含子。该基因编码489个氨基酸残基的蛋白序列,含有一个HMG-box结构域。通过实时荧光定量PCR和转录组测序共同分析表明,该基因在金针菇原基时期的表达量比双核菌丝时期高达4倍以上,具有显著性差异（P<0.01）,由此推测该转录因子参与调控金针菇的原基形成。HMG-box转录因子Fv-Hmg在金针菇原基形成过程中的作用仍需进一步研究。     

一株从高粱蚜分离的镰刀菌的鉴定

一株被误认为是蚜霉(Entomophthora aphidisHoffm)的菌株经鉴定是串珠镰刀菌(Fusariummoniliforme Sheldon)。 “昆虫学报”Vol.9 No 1 刊出“蚜霉菌剂的简易制备及其田间防治棉蚜实验初报”一文,目前该菌已在全国各地推广应用,井已收到防治棉蚜的初步效果。我们对此菌株的分类地位提出异议,经原作者的支持进行了重新鉴定。本文仅记载该菌的形态和培养特性以及学名的鉴定。     

小叶三点金的黄酮类成分研究

从小叶三点金(Desmodium microphyllum)乙醇溶液中分离得到9个黄酮类化合物,通过波谱学方法分别鉴定为()-表儿茶素(1)、genistein-4′-O-β-glucoside(2)、异荭草苷(isoorientin,3)、荭草苷(orientin,4)、木犀草素(luteotin,5)、异日本獐牙菜素(isoswertiajaponin,6)、luteoayamenin(7)、木犀草素-7-O-β-D-葡萄糖苷(luteolin-7-galactoside,8)、木犀草素-7-O-鼠李糖苷(luteolin-7-rhamnoside,9)。所有化合物均为首次在该植物中分离得到。     

Effect of Celecoxib on Ca2+ Fluxes and Proliferation in MDCK Renal Tubular Cells

The effect of celecoxib on renal tubular cells is largely unexplored. In Madin Darby canine kidney (MDCK) cells, the effect of celecoxib on intracellular Ca^{2 +} concentration ([Ca^{2 +}]_i) and proliferation was examined by using the Ca^{2 +}-sensitive fluorescent dye fura-2 and the viability detecting fluorescent dye tetrazolium, respectively. Celecoxib (≥1 μ M) caused an increase of [Ca^{2 +}]_i in a concentration-dependent manner. Celecoxib-induced [Ca^{2 +}]_i increase was partly reduced by removal of extracellular Ca^{2 +}. Celecoxib-induced Ca^{2 +} influx was independently suggested by Mn^{2 +} influx-induced fura-2 fluorescence quench. In Ca^{2 +}-free medium, thapsigargin, an inhibitor of the endoplasmic reticulum Ca^{2 +}-ATPase, caused a monophasic [Ca^{2 +}]_i increase, after which celecoxib only induced a tiny [Ca^{2 +}]_iincrease; conversely, pretreatment with celecoxib completely inhibited thapsigargin-induced [Ca^{2 +}]_i increases. U73122, an inhibitor of phospholipase C, abolished ATP (but not celecoxib)-induced [Ca^{2 +}]_i increases. Overnight incubation with 1 or 10 μ M celecoxib decreased cell viability by 80% and 100%, respectively. These data indicate that celecoxib evokes a [Ca^{2 +}]_i increase in renal tubular cells by stimulating both extracellular Ca^{2 +} influx and intracellular Ca^{2 +} release and is highly toxic to renal tubular cells in vitro.     

中国蝴蝶新种,新亚种及新记录种（I）（鳞翅目）

记述中国蝴蝶１新种、３新亚种、２中国新记录种和２中国新记录亚种,模式标本保存在西北农要科技大学昆虫博物馆。     

Post-juvenile and post-breeding moult of Savi's Warblers Locustella luscinioides in Portugal

We describe the sequence and extent of the complex and little understood post-juvenile and post-breeding moults of Savi's Warblers Locustella luscinioides . In contrast to previous studies, the post-juvenile moult occurred in at least 44% of the birds, 5% of which moulted some or all tertials and greater coverts. The timing of overlap between the filling and the post-juvenile moults, and the fact that later-moulting birds had no post-juvenile moult, strongly suggests that the moult extent is dependent on fledging date. From July onwards, all adult males overlapped breeding and moult, whereas only 11% of the females did so. The start of moult varied from 6 June to 25 August, and was significantly earlier in males. Only 18% of the birds completed the moult, whereas the remaining individuals retained a variable number of inner primaries and/or secondaries. Interestingly, not only was the number of retained primaries positively associated with the date of moult, but so too was the primary number of birds in which the moult started. We view this as an adaptation allowing the replacement of the most important feathers for flight when the time available for moult is short. Body condition did not vary with the progress of moult when date was taken into account, but fat reserves still tended to decrease and then increase. The body condition was correlated positively with the wing raggedness, so Savi's Warblers do not compensate for an increasing wing load during moult.