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41.
Eight new tetramicrosatellite loci for Eurasian otter (Lutra lutra) were designed. Polymorphism of these eight loci in 29 otter individuals was tested. The results indicated that the allele numbers of each of loci ranged from three to five and the observed heterozygosity from 0.483 to 0.828. These new loci can be useful for population genetic research on otters and help improve the resolution of individual identification using noninvasive method.  相似文献   
42.

Background aim

Translation of therapeutic cell therapies to clinical-scale products is critical to realizing widespread success. Currently, however, there are limited tools that are accessible at the research level and readily scalable to clinical-scale needs.

Methods

We herein developed and assessed a closed loop bioreactor system in which (i) a highly gas-permeable silicone material was used to fabricate cell culture bags and (ii) dynamic flow was introduced to allow for dissociation of activated T-cell aggregates.

Results

Using this system, we find superior T-cell proliferation compared with conventional bag materials and flasks, especially at later time points. Furthermore, intermittent dynamic flow could easily break apart T-cell clusters.

Conclusions

Our novel closed loop bioreactor system is amenable to enhanced T-cell proliferation and has broader implications for being easily scaled for use in larger need settings.  相似文献   
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One of the largest environmental assessment programs in the United States was initiated in the early 1990s to determine the chemical characteristics of soil located within the planned alignment for the Central Artery (I-93) / Tunnel (I-90) (CA/T) Project in Boston, Massachusetts. The primary purpose of the program was to support management of the handling and disposal of over 17 million cubic yards of soil to be excavated during construction of the CA/T Project. As part of this work, more than 8,000 soil samples were collected from more than 2,600 soil borings and analyzed for a range of chemical contaminants, including volatile organic compounds, acid/base neutral compounds, total petroleum hydrocarbons, polychlorinated biphenyls, and heavy metals. The soils encountered during the investigations exhibited properties influenced by numerous anthropogenic activities. These activities, such as vehicular emissions, historic industrial/manufacturing operations, and waterfront filling with both building rubble and dredge spoils from Boston Harbor, resulted in soils primarily contaminated with petroleum hydrocarbons and metals. As a result of this program, an extensive database of the chemical constituents present in urban soils in downtown Boston was developed. These results were primarily used to delineate the limits of contaminated areas affecting the planned construction. In addition, the database has been used by the Project to support various soil management activities, as well as by the regulatory community in developing guidelines and criteria governing the management of contaminated soils in Massachusetts. This paper focuses on the various applications of this database throughout the course of the Project, and with the additional aim of stimulating potential future applications by both the regulatory and scientific communities.  相似文献   
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一.引言 亚洲飞蝗(Locusta migratoria manilensis Meyen)(以下简称飞蝗)在我国沿海诸省每年一般发生两代。根据在洪泽湖与微山湖蝗区观察的结果,夏蝗在6月下旬和7月上旬产卵,至同年7月中、下旬孵化;秋蝗在9月上、中旬产卵,到次年6月上旬孵化。越冬蝗卵在土中停留8个月左右的时间,这使它们有充分的机会去领受当地环境因素如寒冷、干,湿、天敌等的作用,从而左右次年飞蝗发生时间  相似文献   
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线粒体是细胞呼吸代谢和能量代谢的中心,有关它的结构和机能是近代生物化学研究中极活跃的领域之一。氧化磷酸化作用,即电子在呼吸链传递过程中偶联有高能磷酸键(腺苷三磷酸ATP)的合成,是线粒体的主要机能,也是研究生物能代谢的重要方面。 氧化磷酸化作用已经在几种昆虫飞翔肌线粒体中被研究过(Sacktor,1961;Slater,1960;Лю Шу-сэнь,1962)。早期研究结果证明,昆虫线粒体与高等动物肝细胞线粒体的区别是前者P/O比值较低,α-甘油磷酸的氧化速率大大高于三羧酸循环各基质的氧化速  相似文献   
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金大雄 《昆虫学报》1975,(3):341-346
1952年1—3月我们在云南大理的树(鼠句)(Tupaia glis Diard)体上采有吸虱,经研究鉴定为一新属、新种,订名为丁Tupaiphthirus dolabrifer。现记述如下。  相似文献   
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