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排序方式: 共有177条查询结果,搜索用时 640 毫秒
71.
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LINDA Y. RUTLEDGE JOSHUA J. HOLLOWAY BRENT R. PATTERSON BRADLEY N. WHITE 《The Journal of wildlife management》2009,73(8):1430-1435
ABSTRACT Sampling of feces for genetic studies of wild populations can be problematic because of the low quality and quantity of template DNA obtained. We used cotton swabs in the field to isolate the mucous layer on the surface of fresh wolf (Canis lupus, C. lycaon, and their hybrids) scats followed by immediate preservation, and compared microsatellite genotyping of DNA from these fresh field swabs (FS) to that of previously frozen laboratory swabs (LS). In single polymerase chain reactions (PCRs) of 2 multiplexes, amplification at 8 loci was higher in the FS samples (FS = 50%, LS = 15%; P = 0.02) because proportion, quantity, and quality of large fragment wolf nuclear DNA from these samples was greater (2.5–25%, 6.25–62.5 ng/swab, 35% amplified at 1,000 base pairs [bp]) than from the LS samples (1.9%–10%, 4.7–25 ng/swab, 10% amplified at 1,000 bp). Paired blood and fresh field-swabbed samples had identical genotypes. In 84 multiplex PCRs we found no evidence of allelic dropout associated with low template quality or quantity. We conclude that field swabbing of fresh wolf scat facilitates field storage and reduces the need for multiple amplifications at single microsatellite loci, thereby reducing the genotyping costs for wildlife projects that use noninvasive samples. 相似文献
73.
SYNOPSIS. Symbiosis between Chlamydomonas hedleyi (Lee, Crockett, Hagen & Stone) and Archais angulatus (Fichtel & Moll) was examined during laboratory studies of primary production and light-enhanced calcification. Photosynthesis and calcification are directly proportional to light intensity in the range of 0–200 μEinsteins m-2 sec-1. Calcification in the light is directly proportional to photosynthesis and proceeds at rates that are 2–3 times that observed in the dark. The herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), in concentrations of 1–100 μM, completely inhibits photosynthesis and light-enhanced calcification. Calcification of the foraminiferan test is therefore due to the photosynthetic activity of the symbiote. Calcification rates for foraminifers incubated in the dark or with DCMU are not significantly different from the calcification rates obtained for dead foraminifers. Rates of calcification obtained with 45Ca are twice that obtained with 14C. 相似文献
74.
We examined changes in the types of fungi consumed by six species of small mammals across a habitat gradient in north‐eastern New South Wales that graded from swamp, to woodland, to open forest and then to rainforest. All mammals ate some fungus, but only bush rats (Rattus fuscipes) regularly did so, and their diet included most of the fungal taxa that we identified across all mammals in the study. The composition of bush rat diet changed significantly with each change in habitat from woodland, to forest, to rainforest. In particular, there was a significant difference in the diets of rats caught either side of the open forest‐rainforest ecotone, which marks the change in fungal community from one dominated by ectomycorrhizal fungi, to a community dominated by arbuscular mycorrhizal fungi. Movement patterns of bush rats living around the open forest‐rainforest ecotone suggest that they transport fungal spores between these contrasting fungal communities. Therefore, bush rats have the potential, by way of spore dispersal, to influence the structure of vegetation communities. 相似文献
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GITA R. KOLLURU GREGORY F. GRETHER SANDRA H. SOUTH ERIC DUNLOP REA CARDINALI LINDA LIU ANDREH CARAPIET 《Biological journal of the Linnean Society. Linnean Society of London》2006,89(2):301-309
Dietary carotenoids have been shown to confer immunological benefits to some species of animals in which males also use these pigments to attract mates. Thus, the potential exists for an allocation trade-off between the sexual and immunological functions of carotenoids. Food availability may also influence immune system function. The present study examined the effects of carotenoid and food availability on the resistance of male guppies ( Poecilia reticulata Peters) from four wild populations to the parasite Gyrodactylus turnbulli Harris. Intermediate levels of carotenoid ingestion resulted in the lowest parasite loads, which suggests that carotenoids strengthen parasite resistance at low levels but either benefit parasites or suppress host immunity at high levels. Males raised on the high-food level initially had fewer parasites, suggesting heightened innate immunity relative to males raised on the low-food level. Over the course of the experiment, however, the high-food males supported higher parasite population growth rates than the low-food males. The results obtained emphasize the importance of evaluating the effects of diet on multiple aspects of immune system function, and caution against assuming that positive effects of carotenoids on immunity in one context will automatically translate to other contexts. © 2006 The Linnean Society of London, Biological Journal of the Linnean Society , 2006, 89 , 301–309. 相似文献
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REBECCA A. SHERRY ENSHENG WENG JOHN A. ARNONE III DALE W. JOHNSON DAVE S. SCHIMEL PAUL S. VERBURG LINDA L. WALLACE YIQI LUO 《Global Change Biology》2008,14(12):2923-2936
Global climate change is expected to result in a greater frequency of extreme weather, which can cause lag effects on aboveground net primary production (ANPP). However, our understanding of lag effects is limited. To explore lag effects following extreme weather, we applied four treatments (control, doubled precipitation, 4 °C warming, and warming plus doubled precipitation) for 1 year in a randomized block design and monitored changes in ecosystem processes for 3 years in an old‐field tallgrass prairie in central Oklahoma. Biomass was estimated twice in the pretreatment year, and three times during the treatment and posttreatment years. Total plant biomass was increased by warming in spring of the treatment year and by doubled precipitation in summer. However, double precipitation suppressed fall production. During the following spring, biomass production was significantly suppressed in the formerly warmed plots 2 months after treatments ceased. Nine months after the end of treatments, fall production remained suppressed in double precipitation and warming plus double precipitation treatments. Also, the formerly warmed plots still had a significantly greater proportion of C4 plants, while the warmed plus double precipitation plots retained a high proportion of C3 plants. The lag effects of warming on biomass did not match the temporal patterns of soil nitrogen availability determined by plant root simulator probes, but coincided with warming‐induced decreases in available soil moisture in the deepest layers of soil which recovered to the pretreatment pattern approximately 10 months after the treatments ceased. Analyzing the data with an ecosystem model showed that the lagged temporal patterns of effects of warming and precipitation on biomass can be fully explained by warming‐induced differences in soil moisture. Thus, both the experimental results and modeling analysis indicate that water availability regulates lag effects of warming on biomass production. 相似文献
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LINDA L. SWEANOR KENNETH A. LOGAN JIM W. BAUER BLUE MILLSAP WALTER M. BOYCE 《The Journal of wildlife management》2008,72(5):1076-1084
Abstract: Because of increasing concerns about puma (Puma concolor) attacks on people and the desire to minimize dangerous puma-human encounters while conserving puma populations, we examined spatial and temporal relationships between pumas and people that used Cuyamaca Rancho State Park (CRSP), California, USA. From 2001 to 2003, we studied 10 adult pumas outfitted with Global Positioning System collars. Although number of visitors to CRSP was increasing, no dangerous puma-human encounters were reported during our study. Male and female pumas typically moved short distances during the day (mean of means of individual hourly movements = 168 m and 131 m each hr, respectively) and moved the most at night (mean of means = 690 m and 390 m each hr, respectively). Of 10 pumas, 9 were least active during the day and most active during the evening or at night. In contrast, most visitor use of trails (x̄ = 85%) occurred during the day. Based on puma and human activity patterns, risk of a puma-human encounter was greatest during the evening. Puma prey caches were randomly distributed in relation to trails and park facilities; however, 8 of 33 caches were still within 100 m of a trail and 2 were within 300 m of a facility. Individual puma behavior relative to human activity areas was variable. Some pumas appeared to temporally avoid human activity areas; others used the park randomly in relation to human activity areas; none appeared to be attracted to human activity areas. Pumas that did not show detectable responses to human activity may have been exhibiting some level of habituation; if so, this level of habituation did not result in puma-human conflicts. When human activity peaked during the day, adult male and female pumas were within 100 m of a trail an average of 9% and 19% of the time they were located in the park, respectively. Thus, there were opportunities for puma-human encounters. Management personnel can take a proactive approach to deal with puma-human interactions through education and protocols that help to minimize probability of conflicts; this may provide the best chance for a continued puma presence in habitat used by pumas and people. 相似文献