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11.
本文用细胞免疫化学方法,在冰冻切片上,检测了胎儿不同组织和器官内带γδ和αβ受体的T细胞(TCR)的分布,结果发现TCR细胞的分布与,般T细胞不同,有相对固定的分布区,如胸腺内TCR细胞主要分布在皮筋质交界处和髓质部;脾脏内的γδT主要位于边缘区,而αβT主要位于动脉周围淋巴鞘,在红髓和血窦两种细胞共存;淋巴结内只有少数TCR细胞位于滤泡间或副皮质,滤泡内则未见。消化管内的TCR细胞主要分布在小肠的固有膜,而胃、大肠和阑尾的固有膜内很少见;肝内TCR细胞主要集中在血管和血窦周围;皮肤切片内的少数TCR细胞见于真皮内,表皮基底层细胞内未见。这些细胞在胎儿期的免疫皮应及其生理功能还不清楚。 相似文献
12.
将γ─亚麻酸生产菌Cunninghamellaspp.8761进行原生质体化,并用物理方法进行诱变,使其在形态上产生了变异,γ─亚麻酸含量有所增加。本文报道了该菌株原生质体的制备,再生条件,以及诱变后形态变化,发酵性能。 相似文献
13.
Yuheng Chen Yongbin Wu Yuran Dong Yao Li Zhiwei Ge Oduro George Gang Feng Lingfeng Mao 《Diversity & distributions》2023,29(2):232-243
Aim
Understanding how species' traits and environmental contexts relate to extinction risk is a critical priority for ecology and conservation biology. This study aims to identify and explore factors related to extinction risk between herbaceous and woody angiosperms to facilitate more effective conservation and management strategies and understand the interactions between environmental threats and species' traits.Location
China.Taxon
Angiosperms.Methods
We obtained a large dataset including five traits, six extrinsic variables, and 796,118 occurrence records for 14,888 Chinese angiosperms. We assessed the phylogenetic signal and used phylogenetic generalized least squares regressions to explore relationships between extinction risk, plant traits, and extrinsic variables in woody and herbaceous angiosperms. We also used phylogenetic path analysis to evaluate causal relationships among traits, climate variables, and extinction risk of different growth forms.Results
The phylogenetic signal of extinction risk differed among woody and herbaceous species. Angiosperm extinction risk was mainly affected by growth form, altitude, mean annual temperature, normalized difference vegetation index, and precipitation change from 1901 to 2020. Woody species' extinction risk was strongly affected by height and precipitation, whereas extinction risk for herbaceous species was mainly affected by mean annual temperature rather than plant traits.Main conclusions
Woody species were more likely to have higher extinction risks than herbaceous species under climate change and extinction threat levels varied with both plant traits and extrinsic variables. The relationships we uncovered may help identify and protect threatened plant species and the ecosystems that rely on them. 相似文献14.
无瓣海桑是广西从自治区外引进的外来红树林树种,采用定量化算法精确估算无瓣海桑地上生物量对红树林生态修复以及海洋蓝碳监测提供经验和方法。论文以广西茅尾海自然保护区无瓣海桑红树林为研究对象,以野外实测无瓣海桑红树林地上生物量数据和Sentinel-1/2卫星提取的后向散射数据、波段数据、植被指数数据和纹理指数数据为数据源,通过分析各遥感因子与实测红树林地上生物量之间的重要性关系,采用极端梯度提升(XGBoost)机器学习算法对比了不同的变量组合对模型精度的影响,最后基于优选的变量组合反演了无瓣海桑红树林的地上生物量。结果表明:(1)研究区无瓣海桑红树林实测树高范围为1.55—13.58m,平均值为8.37m,胸径范围为0.7—41cm,平均值为15.62cm;(2)通过XGBoost算法优选的21个特征变量组合模型拟合效果较好,其模型在测试阶段R2=0.7237,RMSE=21.70Mg/hm2。XGBoost算法反演研究区无瓣海桑地上生物量介于19.14—138.46Mg/hm2之间,平均值为51.92Mg/hm... 相似文献
15.
Ying-Ying Li Xi Chen Jin-Xian Yang Qiang Chen Tie-Ying Song Jun-Qing Ge 《Journal of fish biology》2023,102(1):141-154
Eels are important aquaculture species for which an increasing number of reference genes are being identified and applied. In this study, five housekeeping genes [RPL7 (ribosomal protein L7), 18 S (18 S ribosomal RNA), EF1A (elongation factor 1α), ACTB (β-actin) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase)] were chosen to evaluate their reliability as reference genes for quantitative real-time PCR (qPCR) for the study of Anguilla anguilla. The expression of the selected genes in different eel tissues was determined using qPCR at different growth stages or upon challenge by Anguillid herpesvirus (AngHV), and the expression levels of these genes were then compared and evaluated using the geNorm and NormFinder algorithms. Then, RefFinder was used to comprehensively rank the examined housekeeping genes. Interestingly, the expression of the evaluated housekeeping genes exhibited tissue-dependent and treatment-dependent variations. In different growth periods A. anguilla tissues, the most stable genes were the following: ACTB in mucus; 18 S in skin and kidney; RPL7 in muscle, gill, intestine and brain; EF1A in heart and liver; and GAPDH in spleen. In contrast, in AngHV-challenged A. anguilla tissues, the most stable genes were the following: 18 S in mucus; RPL7 in skin, gill, heart, spleen, kidney and intestine; EF1A in muscle and liver; and ACTB in brain. Further comparison analysis indicated that the expression of RPL7 and EF1A was stable in multiple A. anguilla tissues in different growth periods and in eels challenged by AngHV. Nonetheless, the expression level of GAPDH in eel tissues was lower, and it was unstable in several tissues. These results indicated that the selection of reference genes for qPCR analysis in A. anguilla should be made in accordance with experimental parameters, and both RPL7 and EF1A could be used as reference genes for qPCR study of A. anguilla at different growth stages or upon challenge by AngHV. The reference genes identified in this study could improve the accuracy of qPCR data and facilitate further studies aimed at understanding the biology of eels. 相似文献
16.
寡毛实蝇属三新种:(双翅目:实蝇科) 总被引:1,自引:0,他引:1
本文记述的3新种,即广西实蝇Dacus(Zeugodacus)guangxianus、近黑颜实蝇D.(Zeugodacus)parater和那大实蝇D.(Asiadacus)nadanus,是1982-1985年在南方各地果园中用诱蝇酮(cue-lure)诱获的,寄主不明。所有模式标本均存放于北京农业部植物检疫实验所。在此谨向提供标本的同志致谢。 相似文献
17.
MENG Wuyi LIN ZhengjiongZHOU Yuancong 《中国科学:生命科学英文版》1996,39(6):584-591
Basic phospholipase A2 (BPLA2) from the venom of Agkistrodon halys pallas has a strong ability to hemolyze erythrocytes. The asymmetrical unit of P212121 crystal of BPLA2 contains two molecules. Self-rotation function was used to study the orientation relationship of these two molecules. Cross-rotation and translation functions were then used to determine the orientations and positions of the two molecules in the unit cell. The model building and preliminary structure refinement were carried out. The result shows that the two molecules in the asymmetrical unit of orthorhombic crystal are related by a non-crystallographic 2-fold symmetry axis. 相似文献
18.
The N-terminal signal sequence of glucitol pcrmease of Escherichia Coli (Gut22) and its analogue (Gut22Ana) were synthesized. The analogue had a Pro residue substituting for the His at the 7th position of Gut22 and a Val residue substituting for the Glu at the 10th position. The intrinsic fluorescence emission spectra indicated that the binding of Gut22 with lipid bilayer was much stronger than that of Gut22Ana. The leakage experiments with calcein-loaded liposomes showed that Gut22 strongly perturbed lipid bilayers while Gut22Ana did not. The apparent partition constant of Gut22 for partitioning into phosphatidylserine/phosphatidylcholine bilayers was measured; the effect of membrane potential on the interaction of Gut22 with lipid bilayers was studied and the conformation changes of Gut22 and Gut22Ana upon interacting with liposomes were studied by the method of circular dichroism analysis. 相似文献
19.
20.
木瓜蛋白酶PPAⅢ自水解作用 总被引:1,自引:0,他引:1
木瓜蛋白酶PPAⅢ自水解作用王秀艳,周慧,葛玉斌,李惟(吉林大学分子生物学系,长春130023)PPAⅢ、papain、pepsine、stembromelain等都属于以Cys-SH为活性中心的酶,同种分子之间存在着一种相互切割的趋势,称为酶自水解... 相似文献