全文获取类型
收费全文 | 8527篇 |
免费 | 943篇 |
国内免费 | 1886篇 |
出版年
2024年 | 46篇 |
2023年 | 155篇 |
2022年 | 313篇 |
2021年 | 498篇 |
2020年 | 368篇 |
2019年 | 452篇 |
2018年 | 412篇 |
2017年 | 336篇 |
2016年 | 358篇 |
2015年 | 526篇 |
2014年 | 637篇 |
2013年 | 716篇 |
2012年 | 801篇 |
2011年 | 740篇 |
2010年 | 557篇 |
2009年 | 487篇 |
2008年 | 553篇 |
2007年 | 521篇 |
2006年 | 428篇 |
2005年 | 411篇 |
2004年 | 363篇 |
2003年 | 334篇 |
2002年 | 330篇 |
2001年 | 201篇 |
2000年 | 201篇 |
1999年 | 155篇 |
1998年 | 90篇 |
1997年 | 87篇 |
1996年 | 63篇 |
1995年 | 44篇 |
1994年 | 40篇 |
1993年 | 29篇 |
1992年 | 18篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 8篇 |
1988年 | 10篇 |
1987年 | 7篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 10篇 |
1981年 | 3篇 |
1965年 | 1篇 |
1961年 | 2篇 |
1956年 | 1篇 |
1955年 | 2篇 |
1954年 | 1篇 |
1951年 | 1篇 |
1949年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 270 毫秒
21.
有机硅橡胶固定化细胞进行的生物转化 总被引:3,自引:0,他引:3
有机硅橡胶固定化细胞进行的生物转化潘冰峰,戴学倩,冯青,李祖义(中国科学院上海有机化学研究所,200032)关键词硅橡胶;白地霉;固定化细胞;生物转化近年来,有机化学领域的一个重要进展是用酶或微生物进行生物转化反应。其中研究和应用较多的是碳基还原,特... 相似文献
22.
利福霉素生产菌产生钝化RifSV物质的分离及性质研究 总被引:1,自引:0,他引:1
利福霉素SV(简称RifSV)生产菌──地中海拟无枝菌酸菌在生物合成RifSV过程中,产生一种能钝化自身产物(RifSV)的物质.实验证实,该物质是由谷氨酸、天冬氨酸、赖氨酸及缬氨酸等14种常见氨基酸组成的蛋白质.分子量(MW)约2.5×104D,等电点(PI)为5.7—6.1.在100℃下加热10min,其活性丧失.作用于RifSV的最适pH值范围为7.4—8.6,最适温度为29℃,初步证实该物质是一种酶(暂称利福霉素钝化酶) 相似文献
23.
汉防己甲素(汉甲)及克矽平(Polyvinylpyridine-N-Oxide,PVNO)是目前较为有效的抑制矽肺纤维化的药物。本文研究了其对胶原mRNA水平的影响.斑点杂交实验表明大鼠接尘60天和120天后α1(Ⅰ)及α1(Ⅲ)mRNA水平明显上升,经汉甲或克矽平治疗1个月或3个月后,胶原mRNA水平明显下降。原位杂交结果表明胶原mR-NA银颗粒与细胞性结节和增厚的肺泡壁的成纤维细胞分布重合。汉甲或克矽平治疗后银颗粒数下降。提示汉甲及克矽平对矽肺进程中的胶原基因表达增强有抑制作用。 相似文献
24.
The red alga Porphyra purpurea (Roth) C. Agardh has a life cycle that alternates between shell-boring, filamentous sporophytes and free-living, foliose gametophytes. The significant morphological differences between these two phases suggest that many genes should be developmentally regulated and expressed in a phase-specific manner. In this study, we prepared and screened subtracted complementary DNA (cDNA) libraries specific for the sporophyte and gametophyte of P. purpurea. This involved the construction of cDNA libraries from each phase, followed by the removal of common clones through subtractive hybridization. Sampling of the subtracted libraries indicated that 8–10% of the recombinant colonies in each library were specific for the appropriate phase. Of 20 putative phase-specific cDNAs selected from each subtracted library, eight unique clones were obtained for the sporophyte and seven for the gametophyte. After confirming their phase-specificities by hybridization to gametophyte and sporophyte messenger RNA, these 15 phase-specific cDNAs were sequenced, and the deduced amino acid sequences were used to search protein databanks. Two proteins encoded by the sporophyte-specific cDNAs and two by the gametophyte-specific cDNAs were identified by their similarity to databank entries. 相似文献
25.
5e-tert-Butyl-2e-[4-(substituted-ethynyl)phenyl]-1,3-dithianes with selected functional groups (R) on the ethynyl moiety are potent blockers of the GABA-gated chloride channel measured as inhibitor concentration (IC50) for 4-n-[3H]propyl-1-(4-ethynylphenyl)-2, 6,7-trioxabicyclo[2.2.2]octanebinding to bovine brain membranes. The terminal R substituents were introduced by coupling 5e-tert-butyl-2e-(4-iodophenyl)-1,3-dithiane with HC ≡ CR or 5e-tert-butyl-2e-(4-ethynylphenyl)-1,3-dithiane with XR. The potency of the parent compound (R=H) with an IC50 of 21 μM is equaled or exceeded by up to 7-fold (i.e. IC50 = 3–21 μM) by several carboxylic acids [R = (CH2)nCO2H (n = 0–3), (CH2nOCH2CO2H (n = 1–3) and CH2SCH2 CO2H] and their esters and two phosphonic acids (CH2CH2PO3H2 and CH2OCH2PO3H2) but not their esters. These carboxyl and phosphonic acids (and their salts) include the most potent water-soluble chloride channel blockers known. Conversion to the monosulfones increases activity of the R = H and CH2OH analogs by 1.2- to 3-fold but decreases that of the R = CH2CH2CO2R′ (R′ = H or CH3) derivatives by 3- to 13-fold. Quantitative structure-activity analyses for 44 2-[4-(substituted-ethynyl)phenyl]-dithianes suggests that the principal feature of the R substituent for high activity is its polarizable volume modeled as molecular refractivity, i.e. this substituent is not a well-defined pharmacophore and undergoes a structurally non-specific interaction with the receptor. These observations lay the background for preparing candidate affinity probes. 相似文献
26.
双价外壳蛋白基因植物表达载体的构建及马铃薯转基因植株的鉴定 总被引:10,自引:0,他引:10
在克隆了马铃薯X病毒(PVX)、马铃薯Y 病毒(PVY)和马铃薯卷叶病毒(PLRV)的外壳蛋白基因的基础上,构建同时包含PVX和PVY 与PVY 和PLRV 两个外壳蛋白基因植物表达框架的表达载体,通过农杆菌(Agrobacterium tumefaciens)介导转化烟草(Nicotianatabacum )和生产上常用的几个马铃薯(Solanum tuberosum )优良品种:“Favorita”、“虎头”、“克4”。经PCR检测证明外源基因已整合到植物的染色体上,得到批量转基因植株。在转PVX+PVY 外壳蛋白基因的烟草上接种PVX (5 μg/m L)、PVY(20 μg/m L)病毒,得到有一定抗性的植株 相似文献
27.
Abstract The ecdysteroid levels in hemolymph, ovary, synganglion and whole body of diapausing female Dermacentor niveus were detected by HPLC, and compared with the results of nondiapausing female. It is revealed that the ecdysteroid levels in hemolymph and ovary of diapausing female are similar basically to that of nondiapausing female in the first few days after engorgement. From the 10th day after engorgement, the ecdysteroid levels of diapausing female decreased and even became distinctly lower than that of nondiapausing female. The paucity of ecdysteroids in these individuals would influence the normal development of oocytes. In order to explore the effect of ecdyateroids on the diapausing female, we injected 20-hydroxyecdysone with different dosages at different time into the ticks, and found that after just complete engorgement the injection with large dosages (10000 and 1375 ng/tick) caused death of the ticks. From 10th to 20th day after engorgement the ecdysteroid levels of diapausing female are lower than that of nondiapausing one before oviposition, the injection with certain dosages 50, 70 and 100 ng/tick> of 20E can accelerate vitellogenesis and terminate reproductive diapause, but the amount of eggs produced by them is less than that produced by nondiapausing female. The termination of diapause in female of ixcdid tick by exogenous ecdysteroids is reported for the first time. 相似文献
28.
RGDS肽对大鼠主动脉球囊内膜剥脱后血管壁增殖的影响 总被引:1,自引:0,他引:1
在大鼠主动脉球囊内膜剥脱术后血管壁细胞过度增殖模型上,用合成的血小板膜纤维蛋白原受体(glycoproteinⅡb/Ⅲacomplex,GPⅡb/Ⅲa)拮抗剂RGDS(Arg-Gly-Asp-Ser,50μmol·kg-1·d-1)治疗可有效地抑制损伤血管壁的细胞计数增加和内膜增厚以及血管平滑肌细胞增殖,显著降低其血管组织3H-TdR和3H-Leu的参入增加程度。实验结果提示RGDS肽作为血管成型术的辅佐剂,对于防治血管再狭窄可能具有潜在的临床应用前景。 相似文献
29.
30.