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991.
LI MINGFANG ZHENG XUEQING ZHU YONGQIANG WANG XIANGSHE LIANG SUYU LI LEI WU XINGRONG 《Molecular ecology resources》2006,6(4):1205-1207
Sixteen microsatellite loci were isolated from lychee (Litchi chinensis), all of which exhibited polymorphism (two or three alleles per locus), with levels of heterozygosity ranging from 0.021 to 0.900. These loci can help assess the genetic structure of lychee. 相似文献
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A peptide ranging from residues 229 to 240 (ENESANEGYRFG) of D1 protein was synthesized by stepwise solid-phase method. Resolution enhancement techniques were combined with band curve-fitting procedures to quantitate the FTIR spectra in the amide I' region (1700-1600 cm-1). FTIR analysis showed that DCMU induced drastic structural modification with a relative decrease of the unordered structure and turns, and a substantial increase of α-helix, which indicated that a much more compact structure was formed when DCMU was applied. The results may reflect molecular information for the protective effect of DCMU against photoinhibition. 相似文献
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XU LIANG ZHI‐HE ZHANG LIANG ZHANG WEN‐PING ZHANG FU‐JUN SHEN ZHI YANG RONG HOU WEI HE KUN WEI BI‐SONG YUE 《Molecular ecology resources》2007,7(6):1012-1014
A total of 15 polymorphic tetranucleotide microsatellite loci in the red panda, Ailurus fulgens, were characterized in this study. Based on evaluations of 33 red pandas, the number of observed alleles for each locus ranged from seven to 17 and the expected and observed heterozygosities were 0.412–0.897 and 0.121–0.909, respectively. The mean polymorphic information content was 0.721. These markers would greatly strengthen the utilization of microsatellite tools in genetic variation studies in red panda populations. 相似文献
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以B链羧端去五肽胰岛素(DPI)结构为模型,应用分子置换法对B链N端去二肽(B1~2)C端去五肽(B26~30)胰岛素(DesB1~2DPI)晶体结构进行了研究.DesB1~2DPI晶体单位晶胞中每个结晶学不对称单位包含1个DesB1~2DPI分子.交叉旋转函数和平移函数搜索均找到了明显突出的峰,确定了DesB1~2DPI分子在晶胞中的取向和位置.进一步的三维模型重建和结构精化结果巩固了DesB1~2DPI的分子置换法研究的正确性. 相似文献
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The ovules of Nicotiana tabacum var. macrophylla 8 days after pollination were fixed successively with 2% EDC (1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide) and a mixture solution of paraformaldehyde and glutaraldehyde, then slightly postfixed in 0.5 % osmium solution. UI- trathin sections of 6-, 9- or 12- celled proembryos embedded in Epon 812 resin were stained in an anti-GA MAb and sheep anti-mouse IgG-colloidal gold (10 nm). This MAb specifically recognizes methyl esters of GA7 and GAn. Therefore, it could be used as a probe to localize GA7 and GAn in cells after EDC fixation. The 12-celled proembryo is composed of a 9-celled embryo and a 3-celled suspensor. Wide distribution of GA7 and GAn was observed in all proembryo cells and most organelles at subeellular level, including walls, plasmodesmata, plasma membrane, cytoplasmic matrix, mitochondria, plastids, vacuoles, endoplasmic reticulum and nuclei. Clusters of gold granules were found in nuclear envelope, nucleomatrix, nucleolus, chromosome, cytoplamic matrix. In a region composed of cytoplasmic matrix, a vacuole and a mitochondrium, such concentrated gold granules were particularly obviously observed. There appeared a gradient distribution of GA7 and Gan from embryo cells decreasingly to suspensor cells. GA7 and GAn could be translocated via intercellular walls, plasmodesmata and vesicles between embryo and suspensor. 1he authors suspect the direction of GA translocation in proembryo may be from suspensor to embryo. To author's knowledge, this is the first report to indicate subcellular and gradient distributions of bioactive gibberellins in plant proembryos. 相似文献