Isolation of microsatellite markers from Pinus densiflora Sieb. et Zucc. using a dual PCR technique

We developed seven microsatellite loci from Pinus densiflora using a dual polymerase chain reaction (PCR) technique. Of 186 clones from a library based on suppression PCR, 127 contained microsatellite sequences. Of these, 43 candidates were determined sequences of both flanking regions, and 16 regions from this group were chosen as development markers. Seven of these primer pairs successfully amplified polymorphic single loci among 83 resistant trees against pine wood nematode. The observed heterozygosity of the seven microsatellite markers ranged from 0.247 to 0.843. Mendelian inheritance was confirmed using megagametophytes.     

Research of blastocyte-like structure in chicken

The chicken embryo is a classic model used to investigate embryonic development, gene expression, and tissue differentiation, and is also an important research tool in studying the animal functional genomics. The whole blastoderms of fresh unincubated eggs from White Leghorn chickens were collected with a paper ring, mechanically broken into small pieces and cultured in medium. Then the small pieces would develop into blastocyte-like structures (BLS), which could be facilitated by an addition of fetal bovine serum (FBS) to the primary culture and their diameter was nearly doubled from 12 to 24 h. The additional yolk had no positive effect on the development in the first 12 h but encouraged the BLSs attaching and inner cells differentiating instead in 24 h. The inner cells of the BLS showing a high alkaline-phosphatase activity similar to those in mouse embryonic stem (ES) cells and also expressing a large amount of the specific stage embryonic antigen-1 (SSEA-1) on the surface, which was known to be the characteristic of non-differentiated mouse and avian ES cells, could finally differentiate into nerve-like cells, fibroblast cells and so on in the medium. Leukemia inhibitory factor (LIF) facilitated the cells' proliferation and prevented differentiation in the suspended culture of the BLSs. So we drew the conclusion that the BLS obtained from broken blastoderm can be used to amplify avian ES cells so as to initiate a new method of producing transgenic chickens.     

甘肃草本植物区系的一条分界线

对甘肃产5科161属531种草本植物（不包括栽培属种）进行了鉴定和登记,且对它们进行了统计分析和区系地理研究,从种数与纬度的关系图中得出,北纬36°05′一线为甘肃草本植物区系中的一条可能的分界线。     

四川蝗虫二新种(直翅目:蝗总科)

1985年5—10月作者等在四川省卧龙自然保护区和马边县大风顶自然保护区进行竹类害虫调查,采得危害竹林的卵翅蝗属Caryanda St(?)l,1878一新种;并在整理四川省永川森林病虫防治试验站蝗虫标本时,又发现该属一新种,现记述如下。模式标本保存于缺西师范大学生物系蝗虫研究室。     

秦巴山区蝗虫群落多样性研究

作者于1995～1997年对秦巴山区蝗虫资源进行了调查。采用Shannon-Wiener提出的多样性指数(H')公式、Pielou提出的均匀度(E)公式及Simpson提出的优势度(C)公式,计算群落的多样性、均匀度和优势度。结果表明,不同地区、不同生境、不同海拔高度及不同月份,秦巴山区蝗虫群落的物种数目、多样性指数值、均匀度及优势度指数值均有明显差异。这主要与蝗虫的生物学特性直接有关,另外也受到环境因素的影响。     

中国安徽巢湖瓜德鲁普世二叠异蝎蛉科新类群的发现(昆虫纲,长翅目)

二叠异蝎蛉科是二叠纪长翅目的代表性类群, 也是长翅目的基干类群。二叠纪是长翅目昆虫的繁盛期, 但中国该时期长翅目昆虫化石记录非常稀少, 目前仅报道了1种。本文根据发现于中国安徽省巢湖市瓜德鲁普统银屏组的一枚标本, 建立了1新属1新种––––廖氏巢湖异蝎蛉(Chaohuchorista liaoi gen. et sp. nov.)。该新属的鉴定特征为翅细长, Rs₁和Rs₂合并为1条脉, Rs₄具2分支, M具5分支, M₂具2分支。该新发现增加了中国二叠纪长翅目的多样性。     

广东黑石顶自然保护区植被分类系统与数字植被图 Ⅱ.群丛的分布

作为黑石顶自然保护区植被信息系统的一部分,探讨了黑石顶地区低级植被分类单位即群丛的划分,共划分为30个群丛,以此作为前期有关该自然保护区植被型与群系划分及数字植被图研制工作的补充,并借助有关的地理信息系统(GIS)软件绘制了比例尺为1:10000的群丛分布数字植被图.     

与草菇原基形成相关的过氧化物酶基因VvDyP的结构与表达分析

DyP-type过氧化物酶作为氧化物酶家族中的一员,参与了菌体氧化应激调节反应以及基质降解等过程。本研究从草菇基因组中获得一个DyP-type 过氧化物酶的编码基因,将其命名为VvDyP。对该基因进行结构分析,结果显示草菇的DyP-type 过氧化物酶编码基因全长为 2 333bp,含有8个外显子,7个内含子;开放阅读框长为1 485bp,编码494个氨基酸。通过系统发育分析发现它与灰盖鬼伞以及糙皮侧耳DyP蛋白同源性最高;分析DyP-type 过氧化物酶编码基因在草菇各个时期的表达谱情况并进行荧光定量PCR实验验证发现,草菇的DyP-type过氧化物酶编码基因只在原基中高表达,推测DyP-type过氧化物酶编码基因可以清除过量的活性氧自由基以保证原基的正常形成。     

水栒子的组织培养与快速繁殖

1 植物名称 水枸子(Cotoneaster multiflorus Bunge). 2 材料类别 茎尖和茎段. 3 培养条件 基本培养基为MS.诱导培养基:(1)MS+6-BAl.0 mg-L-1(单位下同)+NAA 0.2;继代培养基:(2)MS+6.BA 0.5+NAA 0.5,(3)MS+6-BA0.1+NAA 0.1;生根培养基:(4)1/2MS+IBA 0.5+NAA 0.1.