中国弯茎叶蝉属一新种记述(同翅目:叶蝉科:片角叶蝉亚科)

本文记述中国弯茎叶蝉属FlexocerusKnohetFang1新种,吴萸弯茎叶蝉Flexocerusevodianussp．nov.,新种外形特征与弯茎叶蝉FlexocerusflexureuskuohetFang相似。模式标本保存在贵州农学院。     

中国东北地区长栉姬蜂属记述（膜翅目：姬蜂科）

本文报道采自我国东北地区的长栉姬蜂属１新种,全黑长栉姬蜂Ｒｈｙｎｃｈｏｂａｎｃｈｕｓ ｎｉｇｅｒ ｓｐ．ｎｏｖ．,２新纪录种,黄斑长栉姬蜂Ｒｈ．ｆｌａｖｏｐｉｃｔｕｓ Ｈｅｉｎｒｉｃｈ,米长栉姬蜂Ｒｈ．ｍｉｎｏｍｅｎｓｉｓ（Ｕｃｈｉｄａ）,并附中国已知种检索表。     

人Ⅳ型胶原的提纯及其抗血清制备

采用胃蛋白酶限制性消化,NaCl分级盐析,还原和烷基化反应,纤维素离子交换层析从人胎盘组织分离纯化Ⅳ型胶原.经SDS-PAGE电泳鉴定符合Ⅳ型胶原α肽链电泳带.用纯化的Ⅳ型胶原免疫兔制备出高效价的特异抗血清.     

PSⅡ反应中心D1蛋白的小肽抗体的制备和鉴定

用人工合成的PSⅡ反应中心D1蛋白中(229～240)的12肽,与牛血清白蛋白偶联后做为抗原免疫家兔, 获得抗菠菜D1蛋白抗体. 免疫双扩散法测得其具较高的效价,蛋白印迹检测结果显示该抗体仅与D1蛋白有免疫亲和反应. 表明此抗体可作为检测D1蛋白及其光抑制时降解产物的探针.     

载脂蛋白AⅡ保护内皮细胞的实验研究

以细胞形态观察、细胞存活率、乳酸脱氢酶释放和前列环素测定等方面研究载脂蛋白AⅡ保护内皮细胞的作用.结果显示,载脂蛋白AⅡ可部分拮抗低密度脂蛋白对内皮细胞的损伤作用, 维持内皮细胞形态基本正常,乳酸脱氢酶释放减少,前列环素合成增加.     

检测脱氨基速率的超灵敏方法及应用

脱氨基被认为是引起细胞突变的主要因素,如果这些脱氨基的产物不被修复,将引起转换(transition)突变.为了理解DNA结构和其化学活性的关系,介绍一种新的灵敏的遗传学方法,它应用在DNA特定点的脱氨基速率的测定.这种方法基于M13mp2噬菌体内的1acZα基因中的CCC脯氨酸密码子的反转突变,即每个脱氨基事件表现为在白色菌斑背景中的一个蓝色菌斑,其灵敏度可达10⁵ M13mp2 DNA分子中检验出一个脱氨基事件.此外,该法可以计算脱氨基动力学速率常数和反应活化能.     

转铁蛋白分型及其基因频率分布

用固相pH梯度(pH 5.05～5.60)等电聚焦技术对随机抽取的188名北京地区健康汉族人的血清转铁蛋白(Tf)进行分型调查,并统计基因频率,检出了在中国还未见报道的Tf^C3基因.其表型分别是：TfC1, TfC2, TfC1C2, TfC1C3, TfC1Dchi, TfC2Dchi.Tf^C1=0.7420, Tf^C2=0.2420, Tf^C3=0.0027, Tf^Dchi=0.0133, 符合Hardy-Weinberg定律, 并与其他已见报道的汉族 Tf基因频率大致相符.     

Sequence analysis of lacZ- mutations induced by ion beam irradiation in double-stranded M13mp18DNA

While M13mp18 double-stranded DNA was irradiated with ion beam, and transfected intoE. coli JM103, a decrease of transfecting activity was discovered. The lacZ^- mutation frequency at 20% survival could reach (3.6–16.8) × 10⁴, about 2, 3–10 times that of unirradiated M13DNA. Altogether, 27 IacZ^-mutants were selected, 10 of which were used for sequencing. 7 of the sequenced mutants show base changes in 250-bp region examined (the remaining 3 mutants probably have base changes outside the regions sequenced). 5 of the base-changed mutants contain more than one mutational base sites (some of them even have 5–6 mutational base sites in 250-bp region examined); this dense distribution of base changes in polysites has seldom been seen in X-rays, Y-rays or UV induced DNA mutations. Our experiments also showed that the types of base changes include transitions(50%), transversions (45%) and deletion (5%); no addition or duplication was observed. The transitions were mainly C→T and A→G; the transversions were mainly C→A and C→G. The mutations involving cytosine residue (in the template strand) constitute about 60% of all the base changes observed. In comparison with the surrounding sequences of mutational base sites, the base located between TG and CT is found to be easily substituted.     

两种吸血水蛭对光闪烁和水扰动刺激的反应

利用自制的控温实验槽在暗室内进行医蛭科两种吸血水蛭对光闪烁和水扰动刺激的模拟个体生态实验,实验结果的统计分析表明,日本医蛭在１６℃时与照度有极好的正相关关系,而在２２℃时成负相关关系,０．２克体重的个体在两种温度对照度的敏感度均大于０．５－０．６克和１．０－１．３克体重的个体,湖北牛蛭在两种温度时与照度均成正相关关系;０．５克体重的个体在１６℃时对照度的敏感度大于１．０－３．３克体重的个体９．５－     

Sublocalization of an Ataxia-Telangiectasia Gene Distal to D11S384 by Ancestral Haplotyping In Costa Rican Families

In an effort to localize a gene for ataxia-telangiectasia (A-T), we have genotyped 27 affected Costa Rican families, with 13 markers, in the chromosome 11q22-23 region. Significant linkage disequilibrium was detected for 9/13 markers between D11S1816 and D11S1391. Recombination events observed in these pedigrees places A-T between D11S1819 and D11S1960. One ancestral haplotype is common to 24/54 affected chromosomes and roughly two-thirds of the families. Inferred (ancestral) recombination events involving this common haplotype in earlier generations suggest that A-T is distal to D11S384 and proximal to D11S1960. Several other common haplotypes were identified, consistent with multiple mutations in a single gene. When considered together with all other evidence, this study further sublocalizes the major A-T locus to ≈200 kb, between markers S384 and S535.