TIBA、LaCl_3、Verapamil和TFP对菜豆下胚轴生长素诱导的导管分化和形成的效应

通过ＴＩＢＡ、ＬａＣｌ３、Ｖｅｒａｐａｍｉｌ和ＴＦＰ对菜豆下胚轴导管分化和形成的调控作用,证明生长素、Ｃａ２＋和ＣａＭ是木质部导管分化和形成所必需的。ＮＡＡ能诱导菜豆下胚轴导管的分化;ＴＩＢＡ能抑制生长素类诱导的导管分化和形成;ＬａＣｌ３和ＴＦＰ在导管分化诱导期（最初２４ｈ）具抑制作用;Ｖｅｒａｐａｍｉｌ与ＴＩＢＡ相似,在导管分化诱导期和导管形成期（伤口形成后７２ｈ之内）都有抑制作用。     

黄瓜幼苗的冷锻炼与低温引起的光抑制

黄瓜幼苗的冷锻炼与低温引起的光抑制李晓萍,陈贻竹,李平,郭俊彦（中国科学院华南植物研究所,广州５１０６５０）关键词：低温引起的光抑制,荧光猝灭,冷锻炼,黄瓜幼苗低温使植物利用光能的能力降低从而引起或加剧光抑制（ｈａｓ等１９８３）。在光抑制中常以叶绿素...     

小牛类表皮生长因子活性肽（c－EGF）的分离纯化及性质

本文报道小牛颌下腺通过酸抽提、ＣＭ５２、ＤＥ５２柱层析、ＳｅｐｈａｄｅｘＧ７５凝胶过滤及ＨＰＬＣ等步,分离纯化到一个分子量为６ｋＤ,等电点为４．６的小肽。该小肽类似人重组表皮生长因子（ｒｈ—ＥＧＦ）的生物活性,具有刺激ＮＲＫ细胞的增殖及刺激新生小鼠的睁眼、萌牙,并具有增加Ａ４３１细胞膜蛋白质磷酸化的功能。氨基酸组成缺少苯丙氨酸及苏氨酸残基。我们命名它为小牛类ＥＧＦ样活性肽（ｃａｌｆＥＧＦ－ｌｉｋｅａｃｔｉｖｅｐｅｐｔｉｄｅ,简称ｃ－ＥＧＦ）。     

酵母醇脱氢酶ADHI的纯化及动力学研究

本文报道了啤酒酵母醇脱氢酶组成型同工酶ＡＤＨＩ的快速高效的纯化方法。通过活性蓝色染料柱亲和层析的方法将该酶纯化至电泳均一,收率达４７％,对该酶的产物抑制及端点抑制动力学研究结果支持Ｗｒａｔｔｅｎ,Ｃｌｅｌａｎｄ提出的序列有序机制。     

Signal transduction pathways in guinea pig sperm

Trifluoperazine (TFP), the antagonist of calmodulin (CaM). significantly stimulated the capacitation and acrosome reaction of guinea pig spermatozoa at the concentration of 10-100μmol/L, independent of the external Ca2+. Forskolin, dbcAMP and caffeine evidently promoted the occurrence of acrosome reaction of spermatozoa at early capacitation stage (5 h) in nonsynchronous system but not in synchronous system. If the spermatozoa were capacitated for 15 h in synchronous system, the above three drugs significantly stimulated acrosome reaction in a Ca2+-independent manner. Protein kinase C activators, i.e. phorbol 12-myristate 13-acetate (PMA) and phorbol 12,13-dibutyrate (PDB) did not influence the occurrence of acrosome reaction of spermatozoa at early capacitation stage, but significantly increased the acrosome reaction rate in capacitated spermatozoa in a Ca2+-independent manner. In contrast. PKC inhibitor staurosporine significantly inhibited the occurrence of acrosome reaction.     

Repair effect of thymine radical anion by echinocoside using pulse radiolysis

Repair activities of thymine radical anion by echinocoside, isolated from Pedicularis plicata. were studied using pulse radiolysis technique. The thymine radical anion was produced by the reaction of hydrated electron with thymine. Echinocoside. one of the polyphenols of phenylpropanoid glycoside, was added to the thymine aqueous solution saturated with N2. Kinetic analysis by transient absorption spectrum showed that thymine radical anion was formed at first, and then after several decades of microseconds of pulse radiolysis. the spectrum of thymine radical anion was changed to that of echinocoside radical anion. The evidence indicated that thymine radical anion was repaired through one-electron-transfer between the DNA base radical anion and echinocoside. The rate constant of electron transfer by echinocoside was 1.45× 109 dm3 · mol1 · s 1.     

Neurobiological mechanisms of the meridian and the propagation of needle feeling along the meridian pathway

The present experiments attempt to find the meridian phenomenon and how the needle feeling propagates along the given meridian channels. The neurobiological mechanisms of the meridian were studied with neuroelectrical recording from the motor neurons and CB-HRP retrograde histochemistry technique in both rats and cats. The results demonstrated that most, but not all, of alpha motor neurons supplying a muscle group of a given meridian were selectively activated by afferent inputs originating not only from homonymous or heterogeneous, but synergistic muscle, but also from the skin nerve overlying the muscle group of the homonymous meridian. However, the afferent inputs from the heterogeneous meridian have very weak or no effect. On the other hand, the labeled motor neurons supplying a given meridian muscles form a discrete longitudinal column with a definite bound in the lateral ventral horn. There are oriented dendro-dendristes projections between the labeled motor neurons.The characteristics of both sel     

下盾螨属一新种──（蜱螨亚纲：厉螨科）

本文记述下盾螨新种Ｈｙｏａｓｐｉｓｓｏｒｅｃｉｓｓｐ．ｎｏｖ,并讨论与近似种Ｈ．ＨｒｙｄｉＳａｍｓｉｎａｋ１９６１的鉴别特征。模式标本采自青海互助土族自治县的暗色ＳｏｒｅｘｓｉｎａｌｉｓＴｈｏｍａｓ,１９１２体上。文内量度单位均为μｍ,标本存放在青海省地方病防治研究所,西宁。     

黄山雄性短尾猴换群行为的研究

１９８６至１９９２年对安徽黄山一群短尾猴连续进行７年的换群行为研究表明：雄性的换群行为发生在临近交配期开始前后,大多数为成年个体;这些个体与换群前的顺位无关,且未受到迁入新群的其它个体更多的攻击,迁入雄体还倍受雌体青睐。据此推测,外群雌性的性吸引是雄性短尾猴换群的直接原因。雄性短尾猴换群的结果,降低了种群的近亲繁殖率,增加了不同种群间的基因交流,对种群遗传多样性的保存和发展具有重要意义．因此,进化选择压力有利于换群行为的发展。     

梅花鹿甲烷能代谢规律的研究

本文应用ＫＢ－１型呼吸测热装置,结合消化、代谢试验,对梅花鹿（Ｃｅｒｖｕｓｎｉｐｐｏｎ）甲烷能代谢规律进行了研究。结果表明,梅花鹿甲烷能的产生量随其采食量的增加而增加;也随着果食后时间的推移而减少,而且减少的幅度又随采食量的增加而下降;甲烷能的产生量分别占总能食入量、消化能食入量和体增热的６．６１％、８．８３％和１０．８８％;甲烷能的产生量随着日粮蛋白质水平的提高而降低,日粮蛋白质水平每提高１个百分点,甲烷能产生量就降低５８．５８ｋＪ／ｄ;分别以总能食入量（ＧＥＩ）和干物质食入量（ＤＭＩ）为自变量所建立的甲烷能（ＣＨ４Ｅ）估计分别为：ＣＨ４Ｅ（ｋＪ／ｄ）＝０．０７ＣＥＪ（ｋＪ／ｄ）－１０１．０４（ｎ＝１２,ｒ＝０．９４４,Ｐ＜０．０１）ＣＨ４Ｅ（ｋＪ／ｄ）＝９８．７８＋１．０５ＤＭＩ（ｇ／ｄ）（ｎ＝１２,ｒ＝０．９４２,Ｐ＜０．０１）