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51.
10μmol/的clotrimazole不仅抑制光合磷酸化活力,而且抑制各种类型的电子传递,是一个典型的电子传递抑制剂。经过它对叶绿体放氧,荧光和毫秒延迟发光影响的比较研究表明:clotri—mazole在光合电子传递链上的作用部位在Q与PQ之间,即与DGMU的作用部位相同或相近。 相似文献
52.
甲基紫精(MV)系统中,在对类囊体膜的光合磷酸化(PSP)活力近于完全抑制的二溴百里香醌(DBMIB)浓度下,由类囊体残缺膜与线粒体嵴膜组成的融合膜PSP活力不仅不被抑制,反而受到不同程度的促进。在铁氰化钾(FeCy)系统中,DBMIB对类囊体膜的PSP活力不能完全抑制,同样浓度的DBMIB对融合膜的PSP活力有抑制效应。检测了不同膜在不同系统中,光下耗氧、放氧、FeCy还原和融合效应的关系等,论证了融合膜中电子传递的途径。 相似文献
53.
In enzymatically dissociated brain cells prepared from neonatal rats, KCl produced a significant increase in [Ca2+]i and this increase could be prevented by verapamil or nifedipine, known to block voltage-sensitive calcium channels. The opioid receptor agonists ohmefentanyl (OMF, mu agonist), [D-Pen2,D-Pen5]enkephalin (DPDPE, delta agonist), and 66A-078 (kappa agonist) produced a marked suppression of the Ca2+ influx induced by high K+ depolarization. The suppressive effect of OMF, DPDPE, and 66A-078 on the high K(+)-induced increase in [Ca2+]i was markedly reversed by their respective antagonists beta-funaltrexamine (beta-FNA), ICI174864, and nor-binaltorphimine (nor-BNI). Cholecystokinin octapeptide (CCK-8), at concentrations of 0.3, 3.0, and 30 nM, dose-dependently mobilized Ca2+ from intracellular stores. While CCK-8 30 nM did not affect significantly the increase of [Ca2+]i following high K+, it did reverse the suppression of the high K(+)-induced increase in [Ca2+]i by the mu agonist OMF and the kappa agonist 66A-078, but not that by the delta agonist DPDPE. The results suggested that while opioid ligands suppress [Ca2+]i by blocking voltage-operated Ca2+ influx, the antiopioid effect of CCK-8 seems to be operated via mobilization of Ca2+ from intracellular stores. 相似文献
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56.
本文分析了我国特产树种云杉Picea asperata的核型,K(2n)=24=20m+4sm,属2A类型,染色体相对长度组成为2n=24=2L+12M_2+SM_1+2S。云杉属植物(22种、变种)的核型全由臂比小于2的中部和近中着丝粒染色体构成,是较为原始的核型。根据松科各属核型的比较,作者讨论了云杉属的亲缘关系和进化地位,并得到形态学、解剖学、孢粉学、植化学、生化学及古植物学等的支持。 相似文献
58.
P. Vuillez F. René M. Plante C. Hindelang M. J. Klein J. M. Félix M. E. Stoeckel 《Cell and tissue research》1992,267(1):169-183
Summary Organotypic cultures, in defined medium, of pituitary primordia obtained from 15-day-old rat fetuses were performed in order to study the in vitro differentiation of melanotrophic cells. The morphological and ultrastructural features of the transplants resembled those of the gland developing in vivo. In situ hybridization on semi-thin sections, using a 35S-labelled oligonucleotide probe, revealed pro-opiomelanocortin-mRNA-containing cells on the first day of culture in the anterior lobe and after 2–3 days in the intermediate lobe. Immunoperoxidase labelling of adjacent sections showed that the same cells reacted with antibodies against -melanocyte-stimulating hormone (MSH), 3 and adrenocorticotropic hormone in both lobes. The pro-opiomelanocortin-mRNA-containing cells formed progressively conspicuous areas in the intermediate lobe, which was almost uniformly labelled after 6 days. In the anterior lobe, these cells remained scattered in small cell groups, and colloidal gold immunolabelling showed the progressive disappearance of MSH labelling from the secretory vesicles in cells exhibiting morphological features of adult corticotrophic cells. Both the MSH content of the explants and MSH release into the culture medium increased with time. Treatment with the dopamine agonist bromocriptine induced a strong dose-dependent decrease in MSH secretion, which was significant after 3 days in culture, indicating that dopamine D2 receptors are able to regulate hormonal release of melanotrophic cells at early stages. This system constitutes a suitable model for further studies of factors controlling cell differentiation and cellular interactions involved in histogenesis. 相似文献
59.
自发性高血压大鼠血管α1肾上腺素受体亚型的改变 总被引:1,自引:0,他引:1
本工作在离体与整体条件下比较易卒中型自发性高血压(SHRSP)大鼠与WKY大鼠血管中α_1受体的两种亚型。在离体灌流的主动脉、肾动脉与肠系膜动脉,50μmol/L氯甲基可乐定(CEC)预温育30min可使α_1受体激动时引起的最大收缩张力在SHRSP与WKY大鼠分别降为对照时的31.4±8.3%与35.2±2.9%,68.4±8.2%与80.1±7.3%,68.4±6.3%与55.4±7.0%,两者间均无显著性差别。但10μmol/L硝苯吡啶对α_1受体收缩效应的阻断作用则在SHRSP大鼠大大超过WKY大鼠,最大收缩张力分别降为对照时的3.1±1.5%与56.5±4.8%(P<0.01),9.0±4.1%与23.6±3.5%(P<0.05),5.9±2.5%与28.0±0.8%(P<0.01)。整体动物实验也显示硝苯吡啶的降血压作用及对苯肾上腺素升血压效应的阻断作用在SHRSP大鼠都较WKY大鼠显著增强。离体主动脉a_1受体激动时的快速相与持续相收缩均主要由α_(1B)亚型激动引起,硝苯吡啶对快速相收缩的阻断作用在SHRSP与WKY大鼠无显著性差别,但对持续相收缩的阻断作用则在SHRSP大鼠显著强于WKY大鼠。上述结果提示SHRSP大鼠血管α_1受体两种亚型的分布没有显著改变,但α_(1B)受体激动时继发性细胞外Ca~(2+)进入的途径由非双氢吡啶敏感性钙通道转变为双氢吡啶敏感性钙通道。 相似文献
60.
Roy E. Crabtree Edward C. Cyr Renée E. Bishop Laura M Falkenstein John M. Dean 《Environmental Biology of Fishes》1992,35(4):361-370
Synopsis Leptocephali were collected in June 1981 and July 1989 over the continental shelf and slope of the Florida west coast. Tarpon larvae ranged 5.5–24.4 mm standard length (SL) and were the second most abundant leptocephalus species. Sagittae examined with compound microscopes and scanning electron microscopy had increments that were presumed to be formed daily. Increment counts made using the two microscopic techniques were not significantly different. Estimated ages ranged 2–25 days with a growth rate (± standard error) of 0.92 ± 0.04 mm d–1 The least squares linear regression equation SL = 2.78 + 0.92 (age in days) best described the relationship between estimated age and length. Adult tarpon appear to undergo a substantial spawning migration from inshore areas frequented during spring and summer to offshore spawning grounds. Spawning occurs during May, June, and July, although the spawning season may be of greater duration. 相似文献