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111.
112.
Highly purified particulate guanylate cyclase from rat lung: characterization and comparison with soluble guanylate cyclase 总被引:2,自引:0,他引:2
Scott A. Waldman John A. Lewicki Ling Y. Chang Ferid Murad 《Molecular and cellular biochemistry》1983,57(2):155-166
Guanylate cyclase was purified 1000-fold from washed rat lung particulate fractions to a final specific activity of 500 nmoles cyclic GMP produced/min/mg protein by a combination of detergent extraction and chromatography on concanavalin A-Sepharose, GTP-agarose, and blue agarose. Particulate guanylate cyclase has a molecular weight of 200 000 daltons, a Stokes radius of 48 A and a sedimentation coefficient of 9.4 while the soluble form has a molecular weight of 150 000 daltons, a Stokes radius of 44 A, and a sedimentation coefficient of 7.0. Whereas the particulate enzyme is a glycoprotein with a specific affinity for concanavalin A and wheat germ agglutinin, the soluble form of guanylate cyclase did not bind to these lectins. Purified particulate guanylate cyclase did not cross-react with a number of monoclonal antibodies generated to the soluble enzyme. While both forms of the enzyme could be regulated by the formation of mixed disulfides, the particulate enzyme was relatively insensitive to inhibition by cystine. With GTP as substrate both forms of the enzyme demonstrated typical kinetics, and with GTP analogues negative cooperativity was observed with both enzyme forms. These data support the suggestion that the two forms of guanylate cyclase possess similar catalytic sites, although their remaining structure is divergent, resulting in differences in subcellular distribution, physical characteristics, and antigenicity. 相似文献
113.
SYNOPSIS Cyclic epidermal cellular prohfeiation,with or withoutkeratinization is a vertebrate characteristic Such activityprobably obeys an autonomous rhythm which is legulated throughneuro humoral S)stcms in response to envnonmental (piox imate)stimuliand related to adaptive (ultimate) factors In seeking causeand effect lelationships, however, it becomes apparent thatthe same environmental parameter may be both an ultimate anda pioximate factor, the latter also regulating the rate of lesponseWith regard to molting in homoio'heims, tempeiatuie acts insuch a capacity in many species Peiiodic shedding of the outer epidermis in fish amphibiansand reptiles does not appear to be coirelated with seasonalfactors to the extent that avian and mammalian molts are The evolution of vertebrate molting cycles has amounted to theentraining of inherent epidermal C)cles with seasonal demandsby the organism itself and the environment,these demands actas regulating mechanisms Pieadapted structures such as feathersand hairs function collectively as plumage and pelage in theirvarious roles but separately in their growth and leplacementcycles which, however, are coordinated for maximum functionalefficiency Molting is also synchionized with the seasonal cycleaccording to the availability of energy resources and time tocomplete the essential functions (in addition to molting) Theevolved molting systems as manifested in the gieat variety ofpatterns and types in the vertebrates, may thus be legardedas almost individual responses to selective piessures actingon a umveisil vertebrate chaiacter The basic regulatoiy system involves the neuro hvpophyseal complexwhich contiols target endocrines affecting various functionswhich themselves influence epidermal mitosis and, ultimately,molting 1 he mechanism in its simplest form controls the animalsmetabolism through the thyroid acting independently in a permissivecapacity or synergistically with the adrenal and gonadal hormoneswhich are regulated directly and/or indirectly through negativefeedback 相似文献
114.
Genetics of somatic fusion in a myxomycete: F 2 studies 总被引:1,自引:0,他引:1
H Ling 《Protoplasma》1971,73(3):407-416
115.
Ling Y. Wei 《Bulletin of mathematical biology》1971,33(2):187-194
Based on quantum transitions of membrane dipoles, the four fundamental properties of nerve impulse are derived in this paper:
the all-or-none response, the strength-duration relation, refractoriness and refractory period and frequency modulation. Furthermore,
the theory offers a physical mechanism for nerve excitation similar to a two-level ammonia maser. It also implies non-threshold
excitation at elevated temperatures. The role of trimethylamine ions near the surface of a phospholipid membrane is briefly
discussed to indicate a possible connection between theory and reality. 相似文献
116.
117.
我国的新蚤属已知有18个种和亚种,其中有12种和亚种属于多毛组,6种属于斯氏组。著者等今整理了从云南、贵州、福建和陕西四省所得到的斯氏组新蚤,除尊新蚤和异种新蚤未获得以外共记述了10种和亚种。其中无规新蚤和福建新蚤为已知种,不同新蚤为我国新记录,均作了补充记述。另外还发现了斯氏新蚤的雌性。本种系Rothschild 氏于1915年根据采自尼泊尔-锡金边境的一只雄蚤而记述。至1932年Jordan氏根据从四川得到的标本记述了雌性形态。本文根据从云南获得的雌雄性标本,重新记述了雌性形态,并由于早先文献中证据的不足和结论的可疑提出Jordan氏1932年记述的雌蚤并非本种,可能是其他种新蚤的雌性。 本文记述了两个斯氏组的新种:1)相关新蚤新种,采自云南中甸 Apodemus agrarius;2)二毫新蚤新种,采自云南中甸 A.agrarius(?E.proditor)。另外,对特新蚤进行了种下分类,提出特新蚤乃一多型物种,根据其种群特征将所得标本分别订为三个地理亚种:(1)云南大理的为特特新蚤;贵阳的为贵阳特新蚤;福建的为闽北特新蚤。 最后编制了我国斯氏组新蚤12个种和亚种的分类检索表。 相似文献
118.
119.
Willy R. G. Baeyens Betty Lin Ling Udo A. Th. Brinkman Stephen G. Schulman 《Luminescence》1989,4(1):484-499
An overview is presented of the physicochemical basis of luminescence, and its application to the detection of chemicals (drugs, biomedically important compounds, environmentally active substances) in liquid chromatographic systems. 相似文献
120.
本文以分离自中国的大量掷孢酵母为材料,研究了不同属的掷孢酵母生掷孢子的能力,以及此能力的持久性,发现属间差异很大。此外还报道了142株不同酵母的产厚垣孢子的情况,发现与担子菌有关的4属酵母在接近致死温度时,都会出现特大厚垣孢子如红掷孢酵母(Sporobolomyces),布勒掷孢酵母(Bullera),红酵母(Rhodotorula)与隐球酵母(cryptococcus),而属于子囊菌的酵母属(Saccharomyces)则无此现象,从而对某些接近担子菌的酵母又增加了新的认识。 相似文献