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122.
HE Xiaolin LIU Xinqi ZENG Zonghao LI Hongmin WANG Miao ZHANG Ying WANG Dacheng 《中国科学C辑(英文版)》2000,43(1):39-46
BmK M4 is a neutral neurotoxin in the BmK toxin series.It is medially toxic and belongs to group III α-toxins.The purified sample was crystallized in rhombic space group P61.Using an X-ray diffraction technique,the crystal structure of BmK M4 was revealed by molecular replacement at 0.20 nm resolution.The model was refined.The final crystallographic R factor was 0.142 and the free R factor was 0.173.The root mean square deviation is 0.001 5 nm for the bond length and 1.753°for the bond angles.64 water molecules were added to the asymmetric unit.The refined structure showed an unusual non-prolyl cis peptide bond at residue 10.The structure was compared with group II α-toxin BmK M8 (an acidic,weak toxin).The potential structural implications of the cis peptide bond were discussed. 相似文献
123.
采用不同浓度的草甘膦0.41 g/L、0.82 g/L、1.23 g/L、1.64g/L、2.05 g/L分别以胃毒和触杀法处理空心莲子草叶甲Agasicles hygrophila成虫,测定其乙酰胆碱酯酶(AChE)、羧酸酯酶(CarE)和谷胱甘肽S-转移酶(GSTs)比活力.试验结果表明:两种处理,草甘膦对AChE活力均有不同程度的抑制作用;对CarE活力影响较为显著,在2.05 g/L浓度下,胃毒处理CarE对α-乙酸萘酯(α-NA)和β-乙酸萘酯(β-NA)水解能力分别是对照组的50%和57%,触杀处理CarE对α-乙酸萘酯(α-NA)和β-乙酸荼酯(β-NA)水解能力分别是对照组的53%和59%;胃毒处理埘酶活力影响大于触杀处理,草甘膦对GSTs的活力影响不明显. 相似文献
124.
Claire E. Reynolds-Peterson Na Zhao Jie Xu Taryn M. Serman Jielin Xu 《Autophagy》2017,13(8):1262-1279
Heparan sulfate-modified proteoglycans (HSPGs) are important regulators of signaling and molecular recognition at the cell surface and in the extracellular space. Disruption of HSPG core proteins, HS-synthesis, or HS-degradation can have profound effects on growth, patterning, and cell survival. The Drosophila neuromuscular junction provides a tractable model for understanding the activities of HSPGs at a synapse that displays developmental and activity-dependent plasticity. Muscle cell-specific knockdown of HS biosynthesis disrupted the organization of a specialized postsynaptic membrane, the subsynaptic reticulum (SSR), and affected the number and morphology of mitochondria. We provide evidence that these changes result from a dysregulation of macroautophagy (hereafter referred to as autophagy). Cellular and molecular markers of autophagy are all consistent with an increase in the levels of autophagy in the absence of normal HS-chain biosynthesis and modification. HS production is also required for normal levels of autophagy in the fat body, the central energy storage and nutritional sensing organ in Drosophila. Genetic mosaic analysis indicates that HS-dependent regulation of autophagy occurs non-cell autonomously, consistent with HSPGs influencing this cellular process via signaling in the extracellular space. These findings demonstrate that HS biosynthesis has important regulatory effects on autophagy and that autophagy is critical for normal assembly of postsynaptic membrane specializations. 相似文献
125.
Species diversity of the genus Osmundea (Ceramiales,Rhodophyta) in the Macaronesian region 下载免费PDF全文
María Machín‐Sánchez Florence Rousseau Line Le Gall Valéria Cassano Ana I. Neto Abel Sentíes Mutue T. Fujii María Candelaria Gil‐Rodríguez 《Journal of phycology》2016,52(4):664-681
Species diversity within the genus Osmundea in the Macaronesian region was explored by conducting a comprehensive sampling in the Azores, the Canary, and the Madeira archipelagos. Toward identification, all specimens were first observed alive to verify the absence of corps en cerise, a diagnostic character for the genus and morphometric data were measured (thallus length and width, first‐order branches length and width, branchlets length and width, cortical cell length and width in surface view, cortical cell length and width in transverse section). Specimens were sequenced for COI‐5P (39 specimens) and three species delimitation methods (Generalized Mixed Yule Coalescent, Automatic Barcode Gap Discovery method, and Poisson Tree Processes) were used to assess the threshold between infra‐ and interspecific relationships. Subsequently, one or several sequences of plastid‐encoded large subunit of RuBisCO (21 specimens) per delimited species were generated to assess the phylogenetic relationships among Macaronesian Osmundea. Moreover, for each delineated species, vegetative and reproductive anatomy was thoroughly documented and, when possible, specimens were either assigned to existing taxa or described as novel species. This integrative approach has provided data for (i) the presence of O. oederi, O. pinnatifida, and O. truncata in Macaronesia; (ii) the proposal of two novel species, O. prudhommevanreinei sp. nov. and O. silvae sp. nov.; and (iii) evidence of an additional species referred as “Osmundea sp.1,” which is a sister taxon of O. hybrida. 相似文献
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127.
Recruitment is a complex process consisting of sequential stages affected by biotic interactions and abiotic factors. Assessment of these sequential stages and corresponding subprocesses may be useful in identifying the most critical stages. Accordingly, to assess the factors that may determine the altitudinal range limits of the high mountain Mediterranean plant Silene ciliata, a set of demographic stages, from flower production to establishment of 2‐yr‐old plants, and their influence on recruitment probability were examined using a step‐by‐step approach. We integrated florivory, pollination and pre‐dispersal seed predation as pre‐dispersal factors, and seedling emergence and survival as post‐dispersal determinants of recruitment. Three populations were monitored at the southernmost margin of the species along its local altitudinal range. Previous studies suggest that seediness is strongly limited by summer drought especially at the lower boundary of the species, a situation that may worsen under current global warming. Our results showed that recruitment was mainly limited by low seed production in the pre‐dispersal stage and low seedling emergence and survival in the post‐dispersal stage, probably due to environmental harshness in summer. By contrast, biotic factors responsible for propagule loss, such as flower and fruit predation, had a minor effect on the probability of plant recruitment. Although the relative importance of transition probabilities was similar among populations along the altitudinal range, comparatively lower flower production significantly reduced the number of recruited plants at the lowest altitude population. This demographic bottleneck, together with increased competition with other species favoured by climate warming, might collapse population growth and limit persistence at the lower altitudinal range of the species, raising its low local altitudinal edge. 相似文献
128.
番荔枝实蝇Ceratitis artortoe(Graham)是一种重要的外来人侵性检疫害虫.在广东口岸,其幼虫连续从入境旅客所携带的水果中被榆出.目前关于番荔枝实蝇潜在适生性分布的研究进行得很少,但对于我国的生物生态安全却有重要意义.本研究中,我们使用3种牛态位模型(ENFA模型,马氏典型性模型和Maxent模型)对番荔枝实蝇在中斟以及全球范尉内的潜在适生性分布区域进行了预测分析.结果显示:Maxent模型拥有最好的预测精确度,马氏典型件模型次之,而ENFA模型的预测精确度最差;Maxent模型和马氏典型性模型的预测精确度无显著性差异;根据Maxent模型的预测结果,番荔枝实蝇在中国的潜在适生区主要是广西、广东、海南以及云南的少部分地区.分析结果显示,番荔枝实蝇从境外传人中国南部地区并最终在上述地区定殖的风险可能性存在,但风险较小.另外,折刀法(Jackknife)分析显示,6种环境因子,例如地面霜冻频率、年平均降雨量、十月降雨量、四月降雨量、年最低温度以及蒸气压,对于番荔枝实蝇在全球和局部地区的分布模式有显著的影响. 相似文献
129.
Threeextrinsicpolypeptides,whicharelocatedontheinnersurfaceofthylakoidmembrane,playessentialrolesinmaintainingthephotosyntheticevolutionofoxygen.Theyaregenerallyrecognizedasliablecomponentsofthephotosyntheticapparatus,andcanbereleasedbyavarietyofphysic… 相似文献
130.
喜马拉雅西部独特、丰富的自然资源对当地居民生计及生态服务等方面起着重要的作用。由于长期以来当地居民与山地生态系统的相互作用,特别是农业生产、畜牧业放牧、薪柴采集以及其他多种多样的资源利用方式,形成了一种特殊的山区文化景观。本文以印度北部的山地小流域PaliGad(共有25个村子)为例,主要研究当地的资源利用状况,利用卫星遥感数据对该地区可利用自然资源进行评估分析,通过从户到户的社会经济调查,对其提供的生态服务功能以及受威胁的程度进行估计,研究分析了村民对资源需求及获取的时空变化情况。结果显示,平均每人每天的薪柴采集量为1.12k,平均每人每天通过修剪枝叶获得饲料采集量为3.69kg,平均每人每天从森林中采集草料的量为3.25kgo对生态系统服务功能进行估测的结果显示,森林可提供更多的临时调节功能,而农业更多的是支撑服务功能,河流,水体给当地人提供了文化服务功能。以山区典型的人-地生态系统为例,这类生态系统中的自然资源破碎化程度很高。研究发现,该区域贫瘠土地上的自然资源需求还在不断增加。因此,从长远来看,人对资源的无止境获取将不利于整个流域的可持续发展。 相似文献