正常大鼠肾脏细胞溶酶体膜的构成蛋白

溶酶体是细胞内对其吞噬之物质溶解及消化之主要场所,同时也是细胞自噬作用的主要细胞器。为了进一步了解此细胞器的功能与结构,我们采用免疫荧光标记法,通过5种针对大鼠肝细胞溶酶体膜蛋白的特异性单克隆抗体,对大鼠正常肾脏细胞溶酶体膜蛋白进行了标记,并通过NH_4Cl溶液对溶酶体作了膜膨胀处理,结果显示:(1)细胞内溶酶体膜蛋白是由多种蛋白所构成,其各种蛋白的含量是不同的;(2)所有溶酶体膜蛋白均表达于该细胞器之表面;(3)NH_4Cl溶液能有效地使溶酶体扩张,这将有和于进一步研究溶酶体的结构。     

普劳螨属一新种记述（蜱螨亚纲：巨须螨科）

本文记述普劳螨属一新种:宽颚普劳螨Pulaeus platygnathus sp.nov.,并与近似种马丁普劳螨Pulaeus martini Den Heyer,1981进行了比较。     

中国长颚属二新种(弹尾目:长颚科)

长颚(虫兆)属(Neanura)1893年由Mac Gillvary所建立,模式种为N.muscorum(Templeton)。该属为一大属,至1964年已记录了75种,5亚种。1967年底,massaud根据口器的结构,对该属进行了订正。他将该属中下颚呈简单的尖刺状,无缨状叶,上颚仅3—4端齿的种保留在该属内,而将上颚6齿以上,下颚复杂,有缨状叶的种列入别的属。经Massaud订正后的长颚(虫兆)属Neanura(s.str)共48种。目前该属已知种已超过55种。     

Depolymerization of cortical microtubules is not a primary cause of chilling injury in corn (Zea mays L. cv Black Mexican Sweet) suspension culture cells

Cold-induced depolymerization of cortical microtubules were examined in suspension culture cells of corn (Zea mays L. cv Black Mexican Sweet) at various stages of chilling. In an attempt to determine whether microtubule depolymerization contributes to chilling injury, experiments were carried out with and without abscisic acid (ABA) pretreatment, since ABA reduces the severity of chilling injury in these cells. Microtubule depolymerization was detectable after 1 h at 4°C and became more extensive as the chilling was prolonged. There was little chilling injury after 1 d at 4°C in either ABA-treated or non-ABA-treated cells. After 3 d at 4°C, there was about 26% injury for ABA-treated and 40% injury for non-ABA-treated cells, as evaluated by 2,3,5-triphenyl-tetrazolium chloride reduction and by regrowth. After 1d at 4°C, less than 10% of cells retained full arrays of microtubules in both ABA-treated and non-ABA-treated cells, the remainder having either partial arrays or no microtubules. After 3d at 4°C, about 90% of cells showed complete or almost complete depolymerization of microtubules in both ABA-treated and non-ABA-treated cells. ABA did not stabilize the cortical microtubules against cold-induced depolymerization. In about 66% of ABA-treated cells and 57% of non-ABA-treated cells that had been held at 4°C for 3d, repolymerization of cortical microtubules occurred after 3h at 28°C. These results argue against the hypothesis that depolymerization of cortical microtubules is a primary cause of chilling injury.     

钉螺体内日本血吸虫尾蚴发育期的形态及其扫描电镜观察

本文通过活体标本、连续切片、全封标本及扫描电镜观察了大陆品系日本血吸虫Schistosomajaponicum子胞蚴体内尾蚴发育期的形态。日本血吸虫尾蚴发育分为五期,即胚细胞期、胚球期、尾蚴雏体期、成熟前期和成熟期。与曼氏血吸虫尾蚴发育进行比较,日本血吸虫尾蚴在胚球期无极化现象;尾蚴雏体期的尾芽形态多非圆球形,尾叉形成较早。钻腺及焰细胞均在此期发生,较曼氏血吸虫尾蚴早。除了上述头器、头腺、钻腺、焰细胞及消化器官进一步发育外,体内散在胚细胞结集为生殖始基,体表感觉乳突分化与体尾肌细胞的发育,尾蚴从成熟前期过渡到成熟期。 电镜首次原位观察尾蚴发育在子胞蚴育腔内的自然状态。尽管偶尔可见到胞蚴体壁与胚元之间某些结构联系,但大部份的胚元各自独立混杂在育腔之中。此外尚注意到尾蚴体棘发生在成熟前期,但其密度较成熟尾蚴小,而其大小却比成熟尾蚴的大。尾蚴头器上感觉乳突和围褶可能先于内部腺体细胞的分化。     

油松毛虫的光周期反应:温度和营养对临界光周的影响

油松毛虫Dendrolimus tabulaeformis Tsai et Liu的临界光周值,在适温区内随温度的下降而增加.不同季节和不同生长阶段的油松针叶,即营养质量不同的针叶,对油松毛虫幼虫的生长、发育和存活影响明显,从而影响着油松毛虫的光周期反应.这些结果,为油松毛虫的世代发生及种群动态的预测提供了一定的依据.     

温度对家白蚁的生存和活动的影响

家白蚁 Coptotermes formosanus Shiraki 的生存、行为和取食活动与温度密切相关。探索温度对家白蚁的生存以及行为、取食诸活动的影响,对于研究其生态学和防治学,都具有重要意义。广东省昆虫研究所对此曾进行过许多有益的研究,并作过一些有价值的报道。笔者于1986—1987年,对此问题作了系统的试验,得到一些新的结果,报道如下。     

中国粘菌Ⅷ.中国新纪录属贫丝菌属Oligonema及其新种:膨丝贫丝菌O.oedonema

贫丝菌属Oligonema是由Rostafinski在1875年建立的,至今仅承认4个种,中国前此尚无记载。作者在长白山采到的这份标本,孢丝极少且短小,中部膨肿,有不甚清晰的螺纹带,具贫丝菌的特征,与已知的种明显不同,因此确认为本属的新成员,定名为膨丝贫丝菌O. oedonema,主模式保存在中国科学院微生物研究所真菌标本室。     

中国寄生云杉的顶裂盘菌及其无性阶段

本文报道了寄生在云杉上的中国新记录种顶裂盘菌(Lophophacidium hyperboreumLagerb.);首次发现了这个种的无性型座壳梭孢属(Apostrasseria sp.),证实了融雪前病株针叶上有表生的菌丝和小菌核;查清了它是新疆云杉林中的广布种,引致云杉雪枯病;发现了新分布区,地理分布范围在75°—94°E,37°40′—49°N;发现了新寄主,多土的西伯利亚云杉(Picea obovata Ledeb.)和引进的青海云杉(P.crassifolia Kom.)、川西云杉(P.balfourianaRehd.et Wils.)。