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51.
The inhibition of ammonium uptake by nitrate in wheat 总被引:1,自引:1,他引:0
52.
G C LEWIS C. RAVEL W. NAFFAA C. ASTIER G. CHARMET 《The Annals of applied biology》1997,130(2):227-238
Infection with endophytic fungi (Acremonium spp.) was detected in wild populations of Lolium spp. from 15 of 20 European countries. Of 523 populations examined, 38% contained no infection, 48% contained 1–50% infection and 14% contained 51–100% infection. Level of infection was slightly but significantly associated with abundance of Lolium in the sward. For data from France, significant correlations were obtained between level of infection and five climate variables; the highest correlations were with evapotranspiration (0.66, P < 0.001) and water supply deficit (-0.66, P < 0.001). A model established using multiple regression analysis and incorporating five climatic variables, accounted for 56% of total variation; water supply deficit alone accounted for 43%. These climatic variables were shown by geostatistical analysis to account for a spatial structure in infection level. Groups of Lolium populations with a high level of infection were located mostly in Mediterranean regions, where stress from summer drought is common. 相似文献
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D. H. LEWIS 《The Journal of eukaryotic microbiology》1975,22(3):344-352
SYNOPSIS. The ultrastructure of promastigotes of 4 reptilian isolates of Leishmania grown in culture, is described. One mammalian isolate of Leishmania is also examined for comparison. No differences in the basic ultrastructure of these strains are apparent; neither is there any significant digression from the organization described for other trypanosomatids. It appears, however, that the numbers of subpellicular microtubules are of potential use in taxonomy, and that differences in the spacing of these organelles exist between reptilian and mammalian forms. In addition, an attempt is made to clarify aspects of attachment of the flagellum to the subpellicular tubule network, and to the anterior face of the kinetoplast. Finally, the formation of multivesiculate bodies from the Golgi apparatus is described, together with some features of dividing forms. 相似文献
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ABSTRACT. Phagocytosis is a highly conserved biological process that serves numerous functions in a wide variety of organisms. Over the past few decades Dictyostelium has proven to be an excellent organism for investigations in cell biology and this is certainly no less the case for a study of phagocytosis. This review examines three distinct phagocytic activities which have been characterized in Dictyostelium. The first, "vegetative phagocytosis," represents the classical eukaryotic microbial uptake of food particles (bacteria). The second, a predatory form of phagocytosis, arises when one species such as Dictyostelium caveatum attacks another species of slime mold, engulfing small pieces of the target prey. This has been termed "cell nibbling." The third phagocytic process is "sexual cannibalistic phagocytosis." In this situation a zygote giant cell, having arisen from the fusion of gametic amoebae, attracts unfused nonzygotic amoebae of the same species and engulfs them as a food source. While cell nibbling has not been actively studied, vegetative and sexual cannibalistic phagocytosis have received varying amounts of attention leading to the idea that some of the elements (e.g., glycoprotein receptors and a Gα s subunit) involved in certain of these phagocytic events may be the same. On the other hand, some unique events (e.g., filopodial induction in prey by D. caveatum ) are also worthy of further investigation. Among other things, the presence of self-nonself recognition, the existence of opsonin-like substances and the presence of signal transduction elements (e.g., an A2-like receptor that negatively modulates sexual phagocytosis) once considered to be extant only in higher organisms suggest that much can be learned about phagocytosis in general by further studies in the classic, eukaryotic microbe Dictyostelium discoideum and related species. 相似文献
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Regenerative Capacity of Visceral Preganglionic Neurones 总被引:1,自引:0,他引:1
P. R. LEWIS PAULA BLUNDELL JONES S. M. BREATHNACH V. NAVARATNAM 《Nature: New biology》1972,236(67):181-182
IT seems to have been widely assumed that autonomic neurones respond to axonal damage much as do somatic motoneurones. From an extensive series of experiments on the albino rat, however, we have obtained evidence that autonomic preganglionic neurones are much more seriously affected. Thus, when the cervical vagus on one side was either crushed or cut, we consistently found in more than 100 animals that changes in the cells of the dorsal motor nucleus of the vagus were more severe and more protracted than in those of the hypoglossal nucleus when its nerve was similarly treated. The changes are seen particularly well in histochemical preparations designed to show the distribution of various metabolic enzymes. In the work described here attention was concentrated on alterations in the cholinesterases and in acid phosphatase. 相似文献
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Plants ofLolium perenne, grown with and without the balansoidfungal leaf endophyteNeotyphodium lolii, were exposed to threeultraviolet radiation treatments at an outdoor facility in theUK for 172 d. Plants were exposed to either (a) a 30% elevationabove the ambient erythemally-weighted level of UV-B (280315nm) radiation under banks of cellulose diacetate filtered fluorescentlamps that also produce UV-A (315400 nm) radiation (UV-B+A);(b) elevated UV-A radiation alone under banks of polyester filteredlamps; or (c) ambient levels of solar radiation under banksof unenergized lamps. The fertility of plants grown withN. loliiwassignificantly reduced by the elevated UV-B+A exposure. After172 d, these plants produced 70% fewer spikes, 75% fewer seeds,71% lower total weight of seed and 78% fewer seeds per g d.wt of plant tissue than plants colonized byN. loliiwhich wereexposed to ambient radiation. There was no discernible effectof elevated UV-B+A exposure on the fertility of endophyte-freeplants. Plants irradiated with UV-B+A developed 14% thickerleaves than those exposed to ambient radiation. Those whichwere irradiated with elevated UV-A alone produced seeds thatwere 20% heavier than plants exposed to ambient levels of radiation.Plants grown withN. loliihad 7% thicker leaves, 4% thicker stembases and 7% fewer tillers than those grown without it. Thefresh mass of tillers of plants grown withN. loliiwas 11% greaterthan those of endophyte-free plants, owing to their higher moisturecontents. These results suggest that the fertility ofL. perennecolonizedbyN. loliiin the natural environment could be deleteriouslyaffected by elevated fluxes of UV-B radiation associated withstratospheric ozone depletion and that this may affect the populationdynamics of the species.Copyright 1998 Annals of Botany Company Fungal leaf endophyte,Neotyphodium lolii, perennial ryegrass (Lolium perenne), stratospheric ozone depletion, UV-B radiation. 相似文献
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G. LEAR I. K. G. BOOTHROYD S. J. TURNER K. ROBERTS G. D. LEWIS 《Freshwater Biology》2009,54(7):1532-1543
1. We set out to evaluate the reliability of bacterial communities as an indicator of freshwater ecological health.
2. Samples of epilithic biofilm were taken over a 1-year period from four streams, each impacted by varying degrees of human modification. The bacteria within each sample were characterised using a whole community DNA fingerprinting technique (automated ribosomal intergenic spacer analysis). Spatial and temporal differences in community structure between samples were visualised using multi-dimensional scaling and quantified using permutational multivariate anova . Macrobenthic invertebrates, which are commonly used as indicators of stream ecological health, were also sampled for comparison.
3. Multivariate analysis revealed a clear gradient in macroinvertebrate community structure between sites exposed to increased human impact. Bacterial communities, however, could only distinguish the most impacted site from the remainder.
4. Additional research is required to increase the sensitivity of bacterial community analyses before endorsing their use as an indicator of freshwater ecological health. 相似文献
2. Samples of epilithic biofilm were taken over a 1-year period from four streams, each impacted by varying degrees of human modification. The bacteria within each sample were characterised using a whole community DNA fingerprinting technique (automated ribosomal intergenic spacer analysis). Spatial and temporal differences in community structure between samples were visualised using multi-dimensional scaling and quantified using permutational multivariate anova . Macrobenthic invertebrates, which are commonly used as indicators of stream ecological health, were also sampled for comparison.
3. Multivariate analysis revealed a clear gradient in macroinvertebrate community structure between sites exposed to increased human impact. Bacterial communities, however, could only distinguish the most impacted site from the remainder.
4. Additional research is required to increase the sensitivity of bacterial community analyses before endorsing their use as an indicator of freshwater ecological health. 相似文献