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The Effect of Methionine Sulphoximine on the Absorption of Ammonium by Maize and Barley Roots over Short Periods 总被引:4,自引:3,他引:1
Pre-treating intact, ammonium-grown plants of maize and barleywith the glutamine synthetase inhibitor, L-methionine sulphoximine(MSO), increased subsequent accumulation of 13N or 15N fromlabelled ammonium very markedlyup to 8-fold in extremecases. The phenonemon could not be satisfactorily explainedin terms of an effect of MSO on the cation-exchange propertiesof the root free space, because neither potassium (86Rb+) norsodium absorption was enhanced by MSO pretreatment, and rapidabsorption of ammonium continued for too long in relation tothe likely free-space content of a monovalent cation. Instead,the response is seen as a consequence of the enlargement ofthe cytoplasmic and vacuolar ammonium pools that occurs whenammonium assimilation is inhibited by MSO. The results, whichincluded comparisons between short-term and longer-term ratesof ammonium absorption, provide tentative evidence of rapidfluxes of ammonium through the plasma membrane. Key words: Cytoplasm, vacuole, flux, transport 相似文献
114.
We re‐examine the higher level phylogeny and evolutionary affinities of the family Gelechiidae (Lepidoptera: Gelechioidea) based on DNA sequence data for one mitochondrial gene (cytochrome c oxidase subunit I) and seven nuclear genes (Elongation Factor‐1α, wingless, Ribosomal protein S5, Isocitrate dehydrogenase, Cytosolic malate dehydrogenase, Glyceraldehyde‐3‐phosphate dehydrogenase and Carbamoylphosphate synthase domain protein). Fifty‐two taxa representing nearly all established subfamilies and tribes of Gelechiidae, and about 10% of described gelechiid genera, in addition to five outgroup taxa were sequenced. Data matrices (6157 bp total) were analysed under model‐based evolutionary methods (Maximum Likelihood and Bayesian Inference), resulting in novel high‐level phylogenetic interrelationships. The best supported cladogram divided the Gelechiidae into six distinct clades corresponding to the subfamilies Anacampsinae, Dichomeridinae, Apatetrinae, Thiotrichinae, Anomologinae and Gelechiinae (+ Physoptilinae, which were not available for study). The results suggest the following adjustments in gelechiid interrelationships: Brachmini is nested within Dichomeridinae; Anarsiini is the sister group of Chelariini; Pexicopiinae is the sister group of Apatetrinae, here suggested to be treated as a tribe Pexicopiini of Apatetrinae. A new subfamily Thiotrichinae ( subfam.n. ) is proposed on the basis of the resurrected genus Thiotricha Meyrick ( gen.rev. ), which includes Macrenches Meyrick, Palumbina Rondani and Polyhymno Chambers. Gelechiidae display a wide array of life‐history strategies, but the diversity in patterns of larval mode of life has direct phylogenetic correlation only below subfamily level, suggesting multiple origins and/or frequent reversals for traits such as external or internal feeding and leaf mining within the family. 相似文献
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DONALD LEE 《The Journal of eukaryotic microbiology》1971,18(2):173-175
SYNOPSIS. The effect of temperature on the stability of lysosomes in homogenates of T. pyriformis at pH 8.1 was studied thruout the temperature range 0–35 C and compared with the stability of lysosomes in homogenates of rat liver and kidney. The lysosomes from T. pyriformis are unstable on exposure for 30 min to temperatures greater than 10–15 C; this instability does not appear to depend on strain, culture medium, growth temperature or the presence or absence of agents which affect the stability of lysosomal membranes. 相似文献
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The family Embolemidae Westwood, 1833 is recorded for the first time in Korea, based on a species Embolemus ruddii Westwood. A diagnosis of this species complemented by digital images are provided. 相似文献
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Muhammad A. KHAN Waseem AKRAM Muhammad ASHFAQ Hafiz A. A. KHAN Yeon K. KIM Jong-Jin LEE 《Entomological Research》2010,40(6):285-289
Silkworm rearing has been an income source for small-holding farmers in Pakistan. Over the years efforts have been made to improve silkworm quality and quantity and thus develop better prospects for increasing yield and income. Mulberry leaves Morus alba L. in a series of experiments have been supplemented with various nutrients in different doses and combinations. Relative success has been achieved in this context. In our present experiments on silkworm Bombyx mori L., we fed the larvae on mulberry leaves supplemented with different doses of N (0.2%), P (0.1%), K (0.3%) and Ca (0.1%) at 26 ± 3°C with 65 ± 5% relative humidity. The results showed better outputs in terms of food consumption (72.55 g/10 larvae), coefficient of utilization (76.45%), body weight (70.07 g/10 larvae) and body length (8.56 cm/larvae) than where simple mulberry leaves were offered. Our net gains with these production parameters have been in terms of heavier cocoons with and without pupa (2.25 and 0.69 g/cocoon), respectively. 相似文献
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