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71.
Spontaneous Transformation of Bovine Lens Epithelial Cells 总被引:2,自引:0,他引:2
Y. COURTOIS L. SIMONNEAU J. TASSIN M. V. LAURENT E. MALAISE 《Differentiation; research in biological diversity》1978,10(1-3):23-30
Bovine lens epithelial cells, in vivo, are known to perform two determined functions. First, they synthesize the lens capsule and subsequently, in the germinal region, they differentiate in fiber cells with massive production of crystallin proteins, inactivation and pyknosis of the nucleus.
Bovine lens epithelial cells from adult origin can be cultured but so far no massive crystallin production has been demonstrated in vitro. We have studied the growth and differentiation of these cells and shown that in long term culture they acquire spontaneously many characteristics of transformation: unlimited growth potential, abnormal karyotype, multilayering. Viral particles were scarcely detected. However, they retain their epithelioid character and the ability to synthesize lens capsule material. Kinetic characteristics of those cells have been determined.
When injected into nude mice, they actively proliferate and form tumors in which synthesis of α-crystallin can be demonstrated. These results show that in vitro transformation of lens epithelial cells does not affect their potential for terminal differentiation. 相似文献
Bovine lens epithelial cells from adult origin can be cultured but so far no massive crystallin production has been demonstrated in vitro. We have studied the growth and differentiation of these cells and shown that in long term culture they acquire spontaneously many characteristics of transformation: unlimited growth potential, abnormal karyotype, multilayering. Viral particles were scarcely detected. However, they retain their epithelioid character and the ability to synthesize lens capsule material. Kinetic characteristics of those cells have been determined.
When injected into nude mice, they actively proliferate and form tumors in which synthesis of α-crystallin can be demonstrated. These results show that in vitro transformation of lens epithelial cells does not affect their potential for terminal differentiation. 相似文献
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Asymmetrical introgression in a freshwater fish hybrid zone as revealed by a morphological index of hybridization 总被引:1,自引:0,他引:1
LAURENT CRESPIN PATRICK BERREBI JEAN-DOMINIQUE LEBRETON 《Biological journal of the Linnean Society. Linnean Society of London》1999,67(1):57-72
Two cyprinid species, Barbus barbus and Barbus meridionalis , form a hybrid zone in the River Lergue (southern France). Hybrids were distinguished morphologically by discriminant function analysis based on samples from the two parental taxa using only three measurements. This morphological hybridization index showed a strong asymmetry in introgression between the two taxa. B. meridionalis can live in the habitats typical of B. barbus and also in habitats where the latter cannot (small upstream tributaries), while remaining essentially unaffected by the hybridization process. By contrast, B. barbus is strongly introgressed because its habitat is invaded at least sporadically by B. meridionalis migrants. There is no behavioral barrier to hybridization, suggesting that an asymmetrical gene flow is probably partly responsible for the asymmetry in introgression. 相似文献
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LAURIANNE LENIAUD ABRAHAM HEFTEZ LAURENT GRUMIAU SERGE ARON 《Biological journal of the Linnean Society. Linnean Society of London》2011,104(4):866-876
In social organisms, the breeding system corresponds to the number of breeders in a group, their genetic relationships, and the distribution of reproduction among them. Recent, genetically based studies suggest an amazing array of breeding system and reproductive strategies in desert ants of the genus Cataglyphis. Using highly polymorphic DNA microsatellites, we performed a detailed analysis of the breeding system and population genetic structure of two Cataglyphis species belonging to the same phylogenetic group: C. niger and C. savignyi. Our results show that both species present very different breeding systems. C. savignyi colonies are headed by a single queen and populations are multicolonial. Remarkably, queens show one of the highest mating frequency reported in ants (Mp = 9.25). Workers can reproduce by both arrhenotokous and thelytokous parthenogenesis. By contrast, colonies of C. niger are headed by several, multiply mated queens (Mp = 5.17), and they are organized in supercolonial populations made of numerous interconnected nests. Workers lay arrhenotokous eggs only. These results illustrate the high variability in the socio‐genetic organization that evolved in desert ants of the genus Cataglyphis. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2011, 104 , 866–876. 相似文献
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HOWARD MOSKOWITZ BARBARA ITTY RACHEL KATZ REA MAIER JACKIE BECKLEY LAURENT FLORES 《Journal of sensory studies》2004,19(6):459-485
Hispanics and non‐Hispanics Americans participated in four sets of studies dealing with health‐oriented foods, with the goal to identify how they respond to food categories relevant in any cuisine. Each study comprised a conjoint analysis using 36 elements followed by a self‐profiling questionnaire to learn more about Hispanic and non‐Hispanic food responses to concepts for four different product categories (morning bread, bottled water, healthful salad dressing and popcorn as a snack). The study identified patterns of preference of marketing statements in the areas of health information, sensory characteristics, emotional benefits and endorsements. Conjoint utility values generated by both Hispanics and non‐Hispanic respondents correlated highly, suggesting that their food attitudes are quite similar for“non‐signature”products despite the differences in their original cuisines. 相似文献
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LIONEL HAUTIER JACQUES MICHAUX LAURENT MARIVAUX MONIQUE VIANEY‐LIAUD 《Zoological Journal of the Linnean Society》2008,154(4):807-821
Graphiurus is a peculiar taxon among the monophyletic Gliridae (order Rodentia) in showing hystricomorphy of the zygomasseteric architecture of the skull [large infraorbital foramen (IOF), and correlative muscular arrangements). We analysed 34 extant genera taken from two groups of sciurognath rodents that share a large IOF (hystricomorph and myomorph) using elliptical Fourier transform in order to appraise whether this feature of cranial morphology was also accompanied by similar changes in mandible shape. The mandible of Graphiurus is distinct from those of all other hystricomorph sciurognath rodents in showing a more elongated coronoid process and a shorter angular process. Thus, two distinct zygomasseteric organizations (i.e. myomorphy and hystricomorphy of graphiurines) are associated with a similar mandible shape characterized by a well‐developed coronoid process. Results show that hystricomorphy of graphiurines was achieved convergently with other hystricomorph rodents. Protrogomorphy is the plesiomorphic condition in Gliridae and hystricomorphy is an autapomorphic feature of Graphiurus. © 2008 The Linnean Society of London, Zoological Journal of the Linnean Society, 2008, 154 , 807–821. 相似文献
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Fatty acids (FAs) profiles and stable isotope signatures of the ghost shrimp, Trypaea australiensis and the soldier crab, Mictyris longicarpus were determined at an unvegetated sandbank of Southport (Gold Coast, Queensland, Australia), in November 2005 and February 2006. Additionally, the FAs composition of the faeces and feeding pellets of M. longicarpus and the surface sediment at the study site were also analysed. Trypaea australiensis was found to selectively feed principally on benthic diatoms, as revealed by the high contribution of the marker lipid (20:5 (n‐3)) to tissue total FAs and the δ13C and δ15N isotopic signatures of shrimp tissues. Although the diet of T. australiensis did not change between the two sampling periods, the shrimps appeared to reduce their feeding activity in summer, presumably in relation to a restricted metabolism, as revealed by a decrease in the contribution of the microalgal markers in their tissues. The FAs composition of the tissues of the soldier crab indicated that bacteria and diatoms constituted the base of its diet (contributions of branched 15:0 and 17:0, 18:1 (n‐7) and 20:5 (n‐3)). However, the isotopic signatures of the crabs suggested that meiofauna may represent an intermediate link between the crab and these micro‐organisms. 相似文献