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41.
A specific decrease in collagen synthesis in acutely fasted, vitamin C-supplemented, guinea pigs 总被引:6,自引:0,他引:6
Weight loss often results from various experimental conditions including scurvy in guinea pigs, where we showed that decreased collagen synthesis was directly related to weight loss, rather than to defective proline hydroxylation (Chojkier, M., Spanheimer, R., and Peterkofsky, B. (1983) J. Clin. Invest. 72, 826-835). In the study described here, this effect was reproduced by acutely fasting normal guinea pigs receiving vitamin C, as determined by measuring collagen and non-collagen protein production after labeling tissues in vitro with [3H]proline. Collagen production (dpm/microgram of DNA) decreased soon after initiating fasting and by 96 h it had reached levels 8-12% of control values. Effects on non-collagen protein were much less severe, so that the percentage of collagen synthesis relative to total protein synthesis was 20-25% of control values after a 96-h fast. These effects were not due to changes in the specific radioactivity of free proline. Refeeding reversed the effects on non-collagen protein production within 24 h, but collagen production did not return to normal until 96 h. The effect of fasting on collagen production was independent of age, sex, ascorbate status, species of animal, and type of connective tissue and also was seen with in vivo labeling. Pulse-chase experiments and analysis of labeled and pre-existing proteins by gel electrophoresis showed no evidence of increased collagen degradation as a result of fasting. Procollagen mRNA was decreased in tissues of fasted animals as determined by cell-free translation and dot-blot hybridization with cDNA probes. In contrast, there was no decrease in translatable mRNAs for non-collagen proteins. These results suggest that loss of nutritional factors other than vitamin C lead to a rapid, specific decrease in collagen synthesis mainly through modulation of mRNA levels. 相似文献
42.
43.
Ernst Wm. Spannhake Terry L. Adams Steven R. Kleeberger 《Prostaglandins & other lipid mediators》1985,30(6):1041-1055
The effects of repeated antigen exposure on the synthesis of mediators by lung tissues are not well understood. To investigate the influence of antigen challenge on the synthesis of prostaglandins by central airway and peripheral lung tissues, fourteen sensitive sheep underwent biweekly exposure to aerosolized antigen (7) or saline (7). Following the fifth exposure, microsomal and high speed supernatant fractions were prepared from trachealis muscle and lung parenchyma. Synthesis of thromboxane (TX) A2, prostaglandin (PG) D2 and PGI2 from the PG endoperoxide intermediate, PGH2, was assayed over a range of substrate concentrations from 3–200 uM. Synthesis of PGI2 by trachealis microsomes was approximately 5-fold greater than that of TXA2. PGI2 and TXA2 production was identical in tracheal preparations from Ascaris- and saline-exposed animals. In parenchymal tissues, where TXA2 production predominated over PGI2 by 9-fold, preparations from Ascaris- exposed animals synthesized 50% more TXA2 than controls at PGH2 concentrations of 25 uM and above, whereas synthesis of PGI2 and PGD2 were similar in preparations from both groups of animals. The density of pulmonary mast cells was decreased by 21% in the Ascaris group, whereas polymorphonuclear leukocyte density was unchanged. These results demonstrate the differential synthesis of TXA2 and PGI2 in central airways and peripheral lung regions of the sheep. They further indicate that repeated exposure of the airways to antigen selectively enhances TXA2 synthesis in the lung periphery of sensitized animals. The site of this increased enzymatic activity, whether in resident cells or newly-infiltrated cells, has not been determined. 相似文献
44.
Inman M. D.; Hughson R. L.; Weisiger K. H.; Swanson G. D. 《Journal of applied physiology》1987,63(4):1578-1585
A mathematical model has been developed that permitted the calculation of the flow-weighted mean tissue O2 consumption (VO2T) at the onset of a step increase in work rate. From breath-by-breath measurements of alveolar O2 consumption (VO2A) and cardiac output (Q) by impedance cardiography and assumptions about the site of depletion of O2 stores, the rate of change in O2 stores (VO2s) was determined. The sum of VO2A + VO2s = VO2T. Six very fit males performed six repetitions of each of two step increases in work rate. STlo was a transition from rest to 100-W cycling; SThi was a transition from 100- to 200-W cycling. For each work rate transition, the responses of VO2A and Q were averaged over the six repetitions of each subject and the model was solved to yield VO2T. The responses of VO2A, VO2T, and Q after the increase in work rate were fit with a monoexponential function. This function included a time constant and time delay, the sum of which gave the mean response time (MRT). In the STlo test, the MRT of VO2A (24.9 +/- 1.1 s, mean +/- SE) was longer than that of VO2T (15.3 +/- 1.3 s) and of Q (16.5 +/- 6.5 s) (P less than 0.05). The MRT of VO2T and Q did not differ significantly. Also for SThi, the MRT of VO2A (34.4 +/- 3.3 s) was significantly longer than that of VO2T (30.0 +/- 3.4 s) (P less than 0.05). The MRT of VO2T and Q (30.3 +/- 5.5 s) were not significantly different at this work rate either.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
45.
V A Berezin E N Zhmarev I A Brodskaia G M Shevchenko L S Zashko 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(7):68-70
The content of neurospecific proteins S-100, GFA and D2 was measured in malignant cerebral tumors by electrophoresis with the use of monospecific antisera. Concomitant measurement of proteins S-100 and GFA is a more reliable diagnostic criterion as to the tumor histogenesis than study of each protein alone. D2 protein appeared to be the most stable specific marker. 相似文献
46.
All plant cells are provided with the necessary rigidity to withstand the turgor by an exterior cell wall. This wall is composed
of long crystalline cellulose microfibrils embedded in a matrix of other polysaccharides. The cellulose microfibrils are deposited
by mobile membrane bound protein complexes in remarkably ordered lamellar textures. The mechanism by which these ordered textures
arise, however, is still under debate. The geometrical model for cell wall deposition proposed by Emons and Mulder (Proc.
Natl. Acad. Sci. 95, 7215–7219, 1998) provides a detailed approach to the case of cell wall deposition in non-growing cells, where there is no evidence for the
direct influence of other cellular components such as microtubules. The model successfully reproduces even the so-called helicoidal
wall; the most intricate texture observed. However, a number of simplifying assumptions were made in the original calculations.
The present work addresses the issue of the robustness of the model to relaxation of these assumptions, by considering whether
the helicoidal solutions survive when three aspects of the model are varied. These are: (i) the shape of the insertion domain,
(ii) the distribution of lifetimes of individual CSCs, and (iii) fluctuations and overcrowding. Although details of the solutions
do change, we find that in all cases the overall character of the helicoidal solutions is preserved. 相似文献
47.
Cristian A. Acevedo Elizabeth Y. Sanchez Juan G. Reyes Manuel E. Young 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(3-4):449-455
It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells. 相似文献
48.
Hostile intercommunity relations, including attacking and killing extra-community infants of both sexes have occurred at most
wild chimpanzee sites. We describe three recent cases of intercommunity attacks on infants committed by members of the Ngogo
chimpanzee community in Kibale National Park, Uganda. Two of the attacks resulted in confirmed infanticides while a third
attack probably resulted in the infant's death. In common with previous accounts of chimpanzee infanticides, the attacks described
here occurred during boundary patrols outside the Ngogo community's usual range, adult and adolescent males were the main
participants, one infant was cannibalized after being killed, and the victims’ mothers did not accompany the attacking party
back to the Ngogo range. However, the patrol parties during each infanticide were larger than before and included females
from the Ngogo community. Our observations indirectly support both the range expansion and imbalance of power hypotheses,
which address why and under which conditions chimpanzee intercommunity encounters lead to aggression. These cases of intercommunity
infanticide add to the growing database of the phenomenon in wild chimpanzees. 相似文献
49.
J.G. Roddick 《Phytochemistry》1974,13(1):9-25
The literature relating to chemical, biochemical and biological aspects of the steroidal glycoalkaloid, α-tomatine, is reviewed. The alkaloid, which can be used as a starting compound for the synthesis of steroidal hormones, is toxic to a wide range of living organisms. The significance of tomatine to plants which elaborate it is discussed and some possible uses of the compound are mentioned. 相似文献