全文获取类型
收费全文 | 722750篇 |
免费 | 81131篇 |
国内免费 | 896篇 |
出版年
2018年 | 6494篇 |
2016年 | 8893篇 |
2015年 | 12637篇 |
2014年 | 14741篇 |
2013年 | 20277篇 |
2012年 | 23543篇 |
2011年 | 23865篇 |
2010年 | 16047篇 |
2009年 | 14579篇 |
2008年 | 21121篇 |
2007年 | 21883篇 |
2006年 | 20481篇 |
2005年 | 19592篇 |
2004年 | 19453篇 |
2003年 | 18432篇 |
2002年 | 18134篇 |
2001年 | 33319篇 |
2000年 | 33540篇 |
1999年 | 26444篇 |
1998年 | 9141篇 |
1997年 | 9306篇 |
1996年 | 8825篇 |
1995年 | 8379篇 |
1994年 | 8074篇 |
1993年 | 7922篇 |
1992年 | 21842篇 |
1991年 | 21534篇 |
1990年 | 20873篇 |
1989年 | 20108篇 |
1988年 | 18646篇 |
1987年 | 17475篇 |
1986年 | 16449篇 |
1985年 | 16188篇 |
1984年 | 13275篇 |
1983年 | 11562篇 |
1982年 | 8632篇 |
1981年 | 7684篇 |
1980年 | 7140篇 |
1979年 | 12579篇 |
1978年 | 9890篇 |
1977年 | 8831篇 |
1976年 | 8200篇 |
1975年 | 9480篇 |
1974年 | 10155篇 |
1973年 | 9927篇 |
1972年 | 8954篇 |
1971年 | 8113篇 |
1970年 | 7067篇 |
1969年 | 6812篇 |
1968年 | 6215篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
952.
Hepatic alcohol dehydrogenase activity and mass content change coordinately during development in male rats. Enzyme activity and mass content increase continuously after birth to 100 and 80% of maximal values within 6 weeks (2.6 ± 0.4 μmole/min/g liver and 92 ± 20 μg/g liver), respectively. When expressed per milligram of soluble proteins, both parameters peak at 3 weeks (0.052 ± 0.002 μmole/min/mg protein and 2.0 ± 0.4 μg/mg protein) and then decrease gradually to plateau levels. These decreases probably arise from a “surge” in soluble liver protein levels that occurs after weaning. Similar developmental patterns also occur in female rats. These findings are the first quantitative measurements of this enzyme in developing animals. 相似文献
953.
L V Loskutova M A Gilinski? 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》1987,37(6):1070-1074
Character of conditioned reaction of passive avoidance was analyzed in Wistar line male rats after neurochemical destruction of terminal dopaminergic fields of the amygdalar complex. 6-hydroxydopamine was bilaterally administered to the central nucleus of the amygdalar complex after preliminary treating with desmethylimipramine for selective destruction of dopaminergic terminals. Lowering of dopamine level in the amygdalar complex led to a weakening of reproduction and to prolongation of spontaneous extinction of conditioned reaction. Features of conditioned reaction are highly similar to the effect of latent inhibition connected with attention deficit. It is suggested that activity of terminal fields of the amygdalar complex is one of the mechanisms providing for attention and intensifying selection of information in learning. 相似文献
954.
955.
The authors analyze the results of comparative studies on 15 paired sera from children with suspected measles, of 32 sera from children and adolescents aged 1.5 to 16 immunized against measles, and of 21 sera from adults aged 19 to 86 with a history of the disease. EIA proved to be more sensitive than HAIT: the detection rate of positive sera was higher, as were the titers of antibodies detected by it, in examinations of the sera from vaccinated children and the adults. Analysis of the distribution of sera with different titers of antibody to measles virus in EIA and HAIT has revealed a correlation between the titers in the sera with high antibody levels. In the cases with low antihemagglutinin titers, no correlation between the titers determined in the two tests has been observed. 相似文献
956.
G G Hillebrand A H McCluskey K A Abbott G G Revich K L Beattie 《Nucleic acids research》1984,12(7):3155-3171
A method has been developed for simultaneous comparison of the propensity of a DNA polymerase to misincorporate at different points on a natural template-primer. In this method elongation of a [5'-32P] primer, annealed to a bacteriophage template strand, is carried out in the presence of only three dNTPs (highly purified by HPLC). Under these conditions the rate of primer elongation (monitored by gel electrophoresis/autoradiography) is limited by the rate of misincorporation at template positions complementary to the missing dNTP. Variations in the rate of elongation (revealed by autoradiographic banding patterns) reflect variations in the propensity for misincorporation at different positions along the template. The effect on primer elongation produced by addition of a chemically modified dNTP to 'minus' reactions reveals the mispairing potential of the modified nucleotide during DNA synthesis. By use of this electrophoretic assay of misincorporation we have demonstrated that the fidelity of E. coli DNA polymerase I varies greatly at different positions along a natural template, and that BrdUTP and IodUTP can be incorporated in place of dCTP during chain elongation catalyzed by this enzyme. 相似文献
957.
S Imajoh H Kawasaki Y Emori K Suzuki 《Biochemical and biophysical research communications》1987,146(2):630-637
Endogenous inhibitors for calcium-activated neutral protease (CANP) were purified from rabbit erythrocytes and liver. The purified inhibitors showed single bands but with significantly different mobilities on sodium dodecylsulfate-polyacrylamide gel electrophoresis. Peptide mapping and sequencing analyses have revealed that the erythrocyte inhibitor (429 residues) retains the C-terminal three repetitive units of the liver inhibitor (639 residues), which contains four potential repetitive units for inhibition of CANP. The erythrocyte and liver inhibitors inhibited 3 and 4 moles of CANP on the basis of the molecular weights of 46,000 and 68,000, respectively. 相似文献
958.
Glycogen debranching enzyme: purification, antibody characterization, and immunoblot analyses of type III glycogen storage disease. 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Type III glycogen storage disease is caused by a deficiency of glycogen debranching-enzyme activity. Many patients with this disease have both liver and muscle involvement, whereas others have only liver involvement without clinical or laboratory evidence of myopathy. To improve our understanding of the molecular basis of the disease, debranching enzyme was purified 238-fold from porcine skeletal muscle. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified enzyme gave a single band with a relative molecular weight of 160,000 that migrated to the same position as purified rabbit-muscle debranching enzyme. Antiserum against porcine debranching enzyme was prepared in rabbit. The antiserum reacted against porcine debranching enzyme with a single precipitin line and demonstrated a reaction having complete identity to those of both the enzyme present in crude muscle and the enzyme present in liver extracts. Incubation of antiserum with purified porcine debranching enzyme inhibited almost all enzyme activity, whereas such treatment with preimmune serum had little effect. The antiserum also inhibited debranching-enzyme activity in crude liver extracts from both pigs and humans to the same extent as was observed in muscle. Immunoblot analysis probed with anti-porcine-muscle debranching-enzyme antiserum showed that the antiserum can detect debranching enzyme in both human muscle and human liver. The bands detected in human samples by the antiserum were the same size as the one detected in porcine muscle. Five patients with Type III and six patients with other types of glycogen storage disease were subjected to immunoblot analysis. Although anti-porcine antiserum detected specific bands in all liver and muscle samples from patients with other types of glycogen storage disease (Types I, II, and IX), the antiserum detected no cross-reactive material in any of the liver or muscle samples from patients with Type III glycogen storage disease. These data indicate (1) immunochemical similarity of debranching enzyme in liver and muscle and (2) that deficiency of debranching-enzyme activity in Type III glycogen storage disease is due to absence of debrancher protein in the patients that we studied. 相似文献
959.
960.