全文获取类型
收费全文 | 784308篇 |
免费 | 85923篇 |
国内免费 | 891篇 |
出版年
2018年 | 6931篇 |
2016年 | 9270篇 |
2015年 | 12983篇 |
2014年 | 15184篇 |
2013年 | 21670篇 |
2012年 | 24507篇 |
2011年 | 25330篇 |
2010年 | 17015篇 |
2009年 | 15535篇 |
2008年 | 22361篇 |
2007年 | 23404篇 |
2006年 | 21964篇 |
2005年 | 21020篇 |
2004年 | 21084篇 |
2003年 | 20115篇 |
2002年 | 19527篇 |
2001年 | 31844篇 |
2000年 | 31815篇 |
1999年 | 25657篇 |
1998年 | 9606篇 |
1997年 | 9890篇 |
1996年 | 9364篇 |
1995年 | 8926篇 |
1994年 | 8584篇 |
1993年 | 8593篇 |
1992年 | 22027篇 |
1991年 | 21703篇 |
1990年 | 21573篇 |
1989年 | 20986篇 |
1988年 | 19608篇 |
1987年 | 18515篇 |
1986年 | 17444篇 |
1985年 | 17753篇 |
1984年 | 14784篇 |
1983年 | 12708篇 |
1982年 | 9790篇 |
1981年 | 8855篇 |
1980年 | 8286篇 |
1979年 | 13971篇 |
1978年 | 11151篇 |
1977年 | 10260篇 |
1976年 | 9700篇 |
1975年 | 10870篇 |
1974年 | 11835篇 |
1973年 | 11597篇 |
1972年 | 10603篇 |
1971年 | 9699篇 |
1970年 | 8310篇 |
1969年 | 8230篇 |
1968年 | 7609篇 |
排序方式: 共有10000条查询结果,搜索用时 406 毫秒
291.
Maria V. Sergeeva Vadim V. Mozhaev Joseph O. Rich Yuri L. Khmelnitsky 《Biotechnology letters》2000,22(17):1419-1422
A novel biocatalytic reaction of transamidation of non-activated amides with amines is reported. Among 45 different lipolytic and proteolytic enzymes tested, only the lipase from Candida antarcticawas able to catalyze this reaction. The reaction proceeded with up to ca. 80% conversion in anhydrous methyl tert-butyl ether and worked with both N-substituted and unsubstituted amides. The biocatalytic transamidation is an equilibrium process and, therefore, higher conversions to the desired amide were achieved by using increased concentrations of the amine nucleophile. 相似文献
292.
293.
294.
295.
Summary The horseradish-peroxidase (HRP) technique was used to visualize the cell bodies of axons projecting to the goldfish pituitary. Following intravenous injections of HRP, HRP reaction products were observed in axons of the rostral pars distalis, proximal pars distalis, neurointermediate lobe, pituitary stalk and in axons coursing from the pituitary into the hypothalamus. HRP-labelled cells in the brain were localized in two regions only — the nucleus preopticus (NPO) pars magnocellularis and pars parvocellularis, and the nucleus lateralis tuberis (NLT) of the hypothalamus. These observations suggest that the NPO and NLT are the source of the neurosecretory innervation of the goldfish pituitary. 相似文献
296.
In the experiments on the anesthesized cats sodium hydroxybutyrate and piracetam, in contrast to glyo-6, have been shown to slow down the growth rate of creatine phosphokinase activity in the blood of the coronary sinus during 60-min occlusion of the coronary artery. At the same time, in the experiments on rats with 3-day myocardial infarction GABA derivatives like glyo-6 failed to influence the final size of cardiac necrosis. It may be concluded that anti-ischemic action of some drugs may be expressed only in the reduction of the rate of ischemic lesion development in the heart, but not in the limitation of the infarction size. 相似文献
297.
W A Van Sickle H G Wilcox A Nasjletti 《Biochemical and biophysical research communications》1986,139(2):416-423
The objective of this study was to determine if the apoprotein or lipid constituents of high density lipoproteins (HDL) mediate HDL-induced prostacyclin synthesis in the Langendorff-perfused rabbit heart. Acetylation, acetoacetylation, or partial removal by trypsin digestion of HDL apoprotein did not reduce the ability of the lipoprotein to stimulate cardiac prostacyclin synthesis. Delipidated apoproteins were less effective in stimulating cardiac prostacyclin synthesis in comparison to intact HDL. In contrast, protein-free lipid vesicles, made from HDL lipids, caused a pronounced stimulation of cardiac prostacyclin synthesis. These results suggest that HDL apoproteins, in their native state, are not essential for HDL-induced cardiac prostacyclin synthesis. The stimulation of cardiac prostacyclin synthesis by HDL may depend on the lipoprotein's lipid rather than on its apoprotein constituents. 相似文献
298.
299.
The primate cochlear nuclear complex exhibits several characteristic morphological differences in the various primate families from Lorisidae through Hominidae. The most striking differences occur in the organization of the dorsal cochlear nucleus in which the laminar pattern becomes progressively obscured. Granule cells form an external granular layer as well as being intermixed within the molecular and pyramidal layers in slow lorises and squirrel and rhesus monkeys. Whereas a prominent external granular layer remains in chimpanzees, granule cells are scant in other portions of the nucleus. Human adults lack an external granular layer. A small number of granule cells occur but with inconstant distribution. Primates lack the linear array of pyramidal cells oriented perpendicularly to the epithelial surface as seen in cats. The granule cell layer exhibits similar regression in development of the human cochlear complex. The external granular layer is prominent in the fetus but rapidly decreases in size after birth. It achieves its adult form prior to 18 months. The data suggest that neuronal attrition, or programmed cell death, may be the major mechanism accounting for the alterations that occur in the human granule cell layer. Other differences in cytoarchitecture, within the great apes and humans, include decreases in the small and giant cell populations of the cochlear complex. These changes, in consort with the organizational changes and reduction of granule cells as noted above, suggest a trend towards reduced intranuclear integration at the level of the cochlear nucleus coupled with encephalization of the auditory system. 相似文献
300.
Avian hepatic T3 generation by 5'-monodeiodination: characterization of two enzymatic pathways and the effects of goitrogens 总被引:1,自引:0,他引:1
F M McNabb L J Lyons T E Hughes 《Comparative biochemistry and physiology. A, Comparative physiology》1986,85(2):249-255
The enzymatic nature of 5'-monodeiodination (5'-D) in avian liver homogenates was demonstrated by abolishment of activity by iopanoic acid (IOP). T3 production from T4 was dependent on enzyme and substrate concentrations, incubation time, incubation temperature, and pH. Two pathways of 5'-D activity were present in avian liver and exhibited characteristics similar to those described in mammalian tissues. Type II activity was identified as propylthiouracil (PTU)-insensitive activity. Type I (PTU-sensitive) was determined by difference between Total and Type II. Km values were 1.58 microM T4 for Total activity and 0.90 nM T4 for Type II, corresponding to the characteristics of the mammalian pathways. The effects of goitrogens on avian hepatic 5'-D were equivalent to those reported for the mammalian enzyme. 相似文献