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811.
812.
Aldi T. Kraja Chunyu Liu Jessica L. Fetterman Mariaelisa Graff Christian Theil Have Charles Gu Lisa R. Yanek Mary F. Feitosa Dan E. Arking Daniel I. Chasman Kristin Young Symen Ligthart W. David Hill Stefan Weiss Jian’an Luan Franco Giulianini Ruifang Li-Gao Fernando P. Hartwig Kari E. North 《American journal of human genetics》2019,104(1):112-138
813.
Roopali Upadhyay Jin Young Kim Eun Young Hong Sun-Gu Lee Joo-Hyun Seo Byung-Gee Kim 《Biotechnology and bioengineering》2019,116(2):i-i
A key point of protein stability engineering is to identify specific target residues whose mutations can stabilize the protein structure without negatively affecting the function or activity of the protein. Here, we propose a method called RiSLnet (Rapid i dentification of Smart mutant Library using residue network) to identify such residues by combining network analysis for protein residue interactions, identification of conserved residues, and evaluation of relative solvent accessibility. To validate its performance, the method was applied to four proteins, that is, T4 lysozyme, ribonuclease H, barnase, and cold shock protein B. Our method predicted beneficial mutations in thermal stability with ~62% average accuracy when the thermal stability of the mutants was compared with the ones in the Protherm database. It was further applied to lysine decarboxylase (CadA) to experimentally confirm its accuracy and effectiveness. RiSLnet identified mutations increasing the thermal stability of CadA with the accuracy of ~60% and significantly reduced the number of candidate residues (~99%) for mutation. Finally, combinatorial mutations designed by RiSLnet and in silico saturation mutagenesis yielded a thermally stable triple mutant with the half-life (T 1/2) of 114.9 min at 58°C, which is approximately twofold higher than that of the wild-type. 相似文献
814.
Chae Rin Park Won Jong Rhee Kyu Won Kim Byeong Hee Hwang 《Biotechnology and bioengineering》2019,116(7):1567-1574
On-site genetic detection needs to develop a sensitive and straightforward biosensor without special equipment, which can detect various genetic biomarkers. Hybridization chain reaction (HCR) amplifying signal isothermally could be considered as a good candidate for on-site detection. Here, we developed a novel genetic biosensor on the basis of enzyme-free dual-amplification of universal hybridization chain reaction (uHCR) and hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme. The uHCR is the strategy which enables simple design for multiple target detection by the introduction of target-specific trigger hairpin without changing the whole system according to a target change. Also, HRP-mimicking DNAzyme could produce a sensitive and quantitative colorimetric signal with increased stability with a limit of detection (LOD) of 5.67 nM. The universality of the uHCR biosensor was proven by the detection of four different targets (miR-21, miR-125b, KRAS-Q61K, and BRAF-V600E) for cancer diagnosis. The uHCR biosensor showed specificity that could discriminate single-nucleotide polymorphism. Moreover, the uHCR biosensor could detect targets in the diluted serum sample. Overall, the uHCR biosensor demonstrated the potential for field testing with a simple redesign without complicated steps or special equipment using a universal hairpin system and enzyme-free amplification. This strategy could enable stable and sensitive detection of a variety of targets. Therefore, it could be applied to urgent detection of various pathogens, remote diagnosis, and self-screening of diseases. 相似文献
815.
Hameed Asif Shahina Mariyam Young Li-Sen Lai Wei-An Sridhar Kandikere Ramaiah Young Chiu-Chung 《Plant and Soil》2019,443(1-2):73-86
Plant and Soil - To screen plant-associated Burkholderia strains for plant probiotic traits including allelochemical metabolism and understand their role on rice allelopathy using a... 相似文献
816.
Hossain Mohammad Tofajjal Khan Ajmal Harun-Or-Rashid Md. Chung Young Ryun 《Plant and Soil》2019,435(1-2):309-321
Plant and Soil - In many mixed grass-shrub ecosystems, increased shrub biomass tends to promote overall carbon storage, but the distribution of carbon pools may be complicated by disturbances such... 相似文献
817.
Mia Petljak Ludmil B. Alexandrov Jonathan S. Brammeld Stacey Price David C. Wedge Sebastian Grossmann Kevin J. Dawson Young Seok Ju Francesco Iorio Jose M.C. Tubio Ching Chiek Koh Ilias Georgakopoulos-Soares Bernardo Rodríguez–Martín Burçak Otlu Sarah O’Meara Adam P. Butler Andrew Menzies Shriram G. Bhosle Michael R. Stratton 《Cell》2019,176(6):1282-1294.e20
818.
819.
Lee Jun-Won Oh Ji Eun Rhee Ki-Jong Yoo Byung-Su Eom Young Woo Park Sang Wook Lee Ji Hyun Son Jung-Woo Youn Young Jin Ahn Min-Soo Ahn Sung-Gyun Kim Jang-Young Lee Seung-Hwan Yoon Junghan 《Molecular and cellular biochemistry》2019,453(1-2):197-203
Molecular and Cellular Biochemistry - Electron transfer occurs through heme-Fe across the cytochrome c protein. The current models of long range electron transfer pathways in proteins include... 相似文献
820.
Young Seung Lee Jae‐Young Chung Sang Bong Jeon Ae‐Kyoung Lee Hyung‐Do Choi 《Bioelectromagnetics》2019,40(7):445-457
This paper proposes a novel in vitro exposure system operating at millimeter‐wave (mmWave) 28 GHz, one of the frequency bands under consideration for fifth generation (5G) communication. We employed the field uniformity concept along cross‐sectional observation planes at shorter distances from the radiation antenna for better efficiency and a small‐size system. A choke‐ring antenna was designed for this purpose in consideration of a wider beamwidth (BW) and a symmetric far‐field pattern across three principal planes. The permittivity of Dulbecco's modified Eagle's medium solution was measured to examine the specific absorption rate (SAR) of the skin cell layer inside a Petri dish model for a three‐dimensional (3D) cell culture in vitro experiment. The best deployment of Petri dishes, taking into account a geometrical field symmetry, was proposed. Local SAR values within the cell layer among the Petri dishes were determined with different polarization angles. It was determined that this polarization effect should be considered when the actual exposure and deployment were conducted. We finally proposed an in vitro exposure system based on the field uniformity including downward exposure from an antenna for 3D cell culture experiments. A small‐size chamber system was obtained, and the size was estimated using the planar near‐field chamber design rule. Bioelectromagnetics. 2019;40:445–457. © 2019 Bioelectromagnetics Society 相似文献