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21.
Shin-ichi Ohnuma Tanetoshi Koyama Kyozo Ogura 《Bioorganic & medicinal chemistry letters》1993,3(12):2733-2738
(E)- and (Z-3-Methyl-3-pentenyl diphosphates acted as artificial substrates in the reaction with geranyl diphosphate catalyzed by solanesyl diphosphate synthase of Micrococcus luteus. The reactions of the E- and Z-isomers proceeded in the same stereochemical manner as that with the natural substrate but stopped at the stage of two steps of condensation, forming C16- and C22-prenyl diphosphates having extra one and two methyl groups at 4- and 4,8-positions, respectively. 相似文献
22.
Elham Jahanmard Kyozo Suyama 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2000,739(2)
Allysine is the most important precursor of physiologically essential cross-links formation in collagen and elastin and is formed by enzymatic oxidative deamination of lysine residues. Because it is a highly reactive aldehyde, many cross-linking amino acid residues may arise from its reaction with other allysine residues or lysine or even histidine residues. We purified and isolated an allysine bisphenol derivative, 1-amino-1-carboxy-5,5-bis-p-hydroxyphenylpentane (ACPP), from the reaction products of phenol and allysine residue of bovine ligamentum nuchae by acid hydrolysis in 6 M HCl. The structure of ACPP was verified by UV, fast atom bombardment-MS, 1H- and 13C-nuclear magnetic resonance spectroscopies. The optimal reaction condition for ACPP synthesis accompanied by hydrolysis of such proteins was investigated and an ion-paired high-performance liquid chromatographic method for determination of allysine as ACPP was also developed. 相似文献
23.
Minoru Yoshida Kyozo Osada Seiji Fujishiro Ryohei Oda 《Bioscience, biotechnology, and biochemistry》2013,77(3):393-400
Procedure of bioassay for chicks was applied to ruminants. Calves of about 8-week-old were fed the same but restricted amount of basal diet and graded levels of corn starch or test materials. The feeding conditions was so adjusted that body weight gain of calves from 1st to 6th week was in proportion to dietary energy supply. Digestive ability of the calves was not disturbed and growth was smooth in the feeding conditions. All of the 3 samples tested were suggested to be available by calves, although palatability of di-lauryl succinate was low. Reliability of the estimate was discussed. 相似文献
24.
Single blastomeres from eight-cell stage bovine embryos matured and fertilized in vitro were electrically fused with enucleated oocytes matured in vitro. In experiment 1, The percentage of these reconstituted embryos developed to the two- to eight-cell stage 48 hr after electrofusion was increased when both the eight-cell embryos and the enucleated oocytes were derived from oocytes cultured with granulosa cells (14% vs. 38%). In experiment 2, the relationship between activation of oocytes and developmental ability of reconstituted embryos was examined. Although both ethanol and electrical stimulation efficiently induced parthenogenetic activation of oocytes matured in vitro for 26–28 hr (ethanol, 89%; electrical stimulation, 73%), the ratio of the second polarbody extrusion differed (80% vs. 22%). Ethanol-treated enucleated oocytes, however, were not significantly different from the early cleavage of the reconstituted embryos 48 hr after electrofusion (nontreated, 38%; treated, 43%). In experiment 3, reconstituted embryos at the two- to eight-cell stage 48 hr after the electrofusion were cocultured with granulosa cells for 6–7 days. Of 69 embryos, one developed to a morula and three developed to blastocysts. © 1993 Wiley-Liss, Inc. 相似文献
25.
Mitsunari Nakajima† Shoei Furukawa† Kyozo Hayashi† Takuji Kawashima Yokichi Hayashi 《Journal of neurochemistry》1993,60(1):267-273
Abstract: A bioassay system originally described by other investigators was improved to evaluate the biological activities of neurotrophic factors (NTFs) and is referred to as a solid-phase bioassay (SPB). The principle and an outline of the SPB are as follows: (a) Test samples containing NTFs are applied to polyacrylamide gel electrophoresis (PAGE) of the sodium dodecyl sulfate (SDS) or two-dimensional type and then transferred onto nitrocellulose membranes, (b) neurons are cultured on the protein-blotted membranes, and (c) the distributions of the surviving neurons are estimated following fixation and clarification of the nitrocellulose membranes. The rationale is that neuron survival will be restricted to the migration positions of the NTF(s). We used nerve growth factor (NGF) as a NTF and sympathetic neurons of 10- to 12-day-old chick embryos as NGF-responsive neurons. Neuronal survival was observed in the areas coinciding with the positions of NGF migration on the membranes in both systems following SDS-PAGE and two-dimensional PAGE. These results indicate that the SPB can identify the positions of NGF-like molecules on nitrocellulose membranes. Using this system, we found active entities, with molecular masses of ∼ 100–200 kDa, different from NGF in crude extracts of mouse submaxillary glands. The SPB developed is considered to be a useful tool for obtaining information on the physicochemical and/or biological properties of putative NTFs in crude samples. 相似文献
26.
27.
Ishikawa S Sekine S Miura N Suyama K Arihara K Itoh M 《Biological trace element research》2004,102(1-3):113-127
The animal biopolymers prepared from hen eggshell membrane and broiler chicken feathers, which are byproducts of the poultry-processing
industry, were evaluated for the removal of the oxyanions selenium [Se(IV) and Se(VI)] and arsenic [As(III) and As(V)] from
aqueous solutions. The biopolymers were found to be effective at removing Se(VI) from solution. Optimal Se(IV) and Se(VI)
removal was achieved at pH 2.5–3.5. At an initial Se concentration of 100 mg/L (1.3 m M), the eggshell membrane removed approx 90% Se(VI) from the solution. Arsenic was removed less effectively than Se, but the
chemical modification of biopolymer carboxyl groups dramatically enhanced the As(V) sorption capacity. Se(VI) and As(V) sorption
isotherms were developed at optimal conditions and sorption equilibrium data fitted the Langmuir isotherm model. The maximum
uptakes by the Langmuir model were about 37.0 mg/g and 20.7 mg/g of Se(VI) and 24.2 mg/g and 21.7 mg/g of As(V) for eggshell
membrane and chicken feathers, respectively. 相似文献
28.
Hirofumi Awatsuji Yoshiko Furukawa Masao Hirota Shoei Furukawa Kyozo Hayashi 《Journal of neurochemistry》1995,64(4):1476-1482
Abstract: Interferon (IFN)-β and IFN-γ inhibited the DNA synthesis and nerve growth factor (NGF) synthesis in growing astrocytes cultured from neonatal mouse brain, but they did not affect the NGF synthesis in quiescent astrocytes. IFN-β and IFN-γ also inhibited the enhanced DNA synthesis and NGF synthesis in growing astrocytes after the administration of basic fibroblast growth factor. These results indicated that NGF synthesis in astrocytes is regulated by IFNs associated with cell growth. The mechanism of IFN action on NGF synthesis/secretion is unknown, but the results that their effects last long after IFN removal from the cultures present the possibility that IFNs destabilize NGF mRNA. 相似文献
29.
† Yoshihide Hashimoto †Shoei Furukawa ‡Fumio Omae Yukio Miyama †Kyozo Hayashi 《Journal of neurochemistry》1994,63(1):326-332
Abstract: Effects of thyroxine (T4 ) on nerve growth factor (NGF) level and choline acetyltransferase (ChAT) activity of rat brains were investigated. Repetitive intraperitoneal administration of T4 caused increases in both NGF level and ChAT activity in the frontal cortex, septum, hippocampus, and striatum and decreases in the cerebellum in 2-day-old rats. Only ChAT activity was elevated in the olfactory bulb, and the NGF level remained unchanged there. No changes were observed in the midbrain and pons/medulla. Furthermore, T4 was effective on the post-natal rats only up to day 11. These results suggest that T4 plays a role in the developmental regulation of NGF level and ChAT activity in rat brain in a region- and/or stage-specific manner. That (1) changes in NGF level and ChAT activity occurred in regions nearly identical to those that contained NGF-responding neurons, and (2) the change in NGF level in the hippocampus and frontal cortex was followed by the change of ChAT activity after a single injection of T4 suggest that the effects of T4 on cholinergic differentiation are, at least in part, mediated via NGF, which itself is quantitatively regulated by T4 . 相似文献
30.
Toshiya Endo Masanao Oya Kyozo Hayashi Tatsuo Miyazawa 《Journal of Protein Chemistry》1989,8(4):575-581
Temperature dependence of proton nuclear magnetic resonance spectra has been followed for cobrotoxin, a postsynaptic neurotoxin fromNaja naja atra venom. Several aromatic amino-acid residues, including the functionally essential Trp-29 located at the tip of the central loop of the molecule, have been found to undergo a thermal structural transition above the global thermal denaturation temperature. It is suggested that a local structure around these residues behaves somehow independently of the rest of the molecule, and that such structural organization may be favorable for a conformational change of a neurotoxin molecule on binding to acetylcholine receptor. 相似文献