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71.
A fibronectin-binding protein from rice bran with cell adhesion activity for animal tumor cells. 总被引:1,自引:0,他引:1
Y Shoji T Mita M Isemura T Mega S Hase S Isemura Y Aoyagi 《Bioscience, biotechnology, and biochemistry》2001,65(5):1181-1186
A rice bran 57-kDa protein was isolated by affinity chromatography with fibronectin immobilized on agarose. This fibronectin-binding protein designated as RB-57 had an amino-terminal amino acid sequence identical with that of a putative mature form of rice hydroxyproline-rich glycoprotein. A distinct feature of the amino acid composition of RB-57 was the high contents of hydroxyproline and proline representing about 45% of the total amino acids. The sugar analysis indicated that arabinose represented 46.8% of the total carbohydrates. RB-57 showed cell adhesion activity for murine Lewis lung carcinoma cells. The result suggests that RB-57 may play a role in plant cell adhesion, although cell adhesion-promoting activity for plant cells remains to be tested. 相似文献
72.
T Niidome H Murakami M Kawazoe T Hatakeyama Y Kobashigawa M Matsushita Y Kumaki M Demura K Nitta H Aoyagi 《Bioorganic & medicinal chemistry letters》2001,11(14):1893-1896
We have designed and synthesized of carbohydrate-binding peptides, gramicidin S analogues. Asn/Asp/Gln and Trp residues in the peptides were employed as the binding sites for carbohydrates by hydrogen-bonding interaction and the creation units for hydrophobic pocket to promote the interaction, respectively. The data of fluorescence spectroscopy and affinity column chromatography indicated that the peptides possessed the binding ability for some carbohydrates in aqueous medium. As a result of 1H NMR study, nuclear Overhauser effects between aromatic side chains of a peptide, [Gln(1,1'),Trp(3,3')]-gramisidin S and mannose were observed, indicating that the interaction of the peptide with the sugar occurred in the hydrophobic environment formed by Trp and Phe residues. 相似文献
73.
HMGB1‐induced inflammatory response promotes bone healing in murine tooth extraction socket
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Villeneuve M Kawai M Watanabe M Aoyagi Y Hitotsuyanagi Y Takeya K Gouda H Hirono S Minnikin DE Nakahara H 《Biochimica et biophysica acta》2007,1768(7):1717-1726
Phase diagrams of Langmuir monolayers of oxygenated mycolic acids, i.e. methoxy mycolic acid (MeO-MA), ketomycolic acid (Keto-MA), and artificially obtained deoxo-mycolic acid (deoxo-MA) from Mycobacterium bovis BCG were obtained by thermodynamic analysis of the surface pressure (pi) vs. average molecular area (A) isotherms. At lower temperatures and lower surface pressures, both Keto- and MeO-MAs formed rigid condensed monolayers where each MA molecule was considered to be in a 4-chain form, in which the three carbon chain segments due to bending of the 3-hydroxy aliphatic carboxylate chain and the 2-side chain were in compact parallel arrangement. At higher temperatures and surface pressures, MeO-MA and deoxo-MA tended to take stretched-out conformations in which the 3-hydroxy aliphatic carboxylate chain was more or less in an extended form, but Keto-MA retained the original 4-chain structure. The thickness measurement of the monolayers in situ by ellipsometry at different pi values and temperatures supported the above conclusions derived from the phase diagrams. The enthalpy changes associated with the phase transitions of MeO-MA and deoxo-MA implied that the MeO-MA needed larger energy to change from a compact conformation to an extended one, possibly and partly due to the dehydration of the methoxy group from water surface involved. Molecular dynamics studies of MA models derived from Monte Carlo calculations were also performed, which confirmed the conformational behavior of MAs suggested by the thermodynamic studies on the Langmuir monolayers. 相似文献
78.
Kazunori Taguchi Yoshihiro Aoyagi Hiromi Matsusaki Toshiaki Fukui Yoshiharu Doi 《Biotechnology letters》1999,21(7):579-584
A Pseudomonas sp. 61-3 malonyl-CoA-ACP transacylase gene (fabD
Ps) was cloned by Southern analysis using an equivalent gene of Escherichia coli (fabD
Ec) as a probe. Some recombinant E. coli HB101 strains harboring fabD
Ps, fabD
Ec, or E. coli 3-ketoacyl-ACP synthase III gene (fabH
Ec) with Aeromonas caviae polyhydroxyalkanoate synthase gene (phaC
Ac) were constructed and grown on one-stage cultivation in Luria-Bertani broth containing glucose as carbon source. These strains accumulated 5 to 11 wt% of poly (3-hydroxybutyrate) (PHB) within cells. Over-expression of fabH
Ec, fabD
Ec, or fabD
Ps has been suggested to lead the monomer-supply of (R)-3-hydroxybutyryl-CoA for PHB synthesis in E. coli cells. 相似文献
79.
The effects of alginate on the physiological activities of plant cells were studied. Addition of alginate oligomer (AO) to
the suspension culture of Catharanthus roseus L. or Wasabia japonica cells promoted the production of antibiotic enzymes such as 5′-phosphodiesterase or chitinase respectively. Ajmalicine (a
secondary metabolite) production by C. roseus CP3 cells was also promoted when AO was added to the suspension culture. On the basis of these results, we assumed that alginate
is an elicitor-like substance. We therefore compared the effect of AO on C. roseus L. and W. japonica cells with those of chitosan oligomer (CO) and oligo-galacturonic acid (OGA), which are well known as an exogenous elicitor
and endogenous elicitor respectively. The effects of various concentrations of AO, OGA, and CO on the physiological activities,
membrane permeability and protoplast formation of C. roseus L. or W. japonica cells were investigated. AO and OGA showed similar physiological effects, which were quite different from those of CO. Since
alginate appeared to have similar effects to galacturonic acid, we concluded that alginate acts as an endogenous elicitor.
Both alginate and galacturonic acid are uronic acids, and we considered their structural similarity. The effects of esterification
of the carboxylic groups of alginate by propylene oxide were also studied. The greater the degree of esterification, the less
the secretion of 5′-phosphodiesterase. Hence we assumed that carboxylic groups have an important role in the initiation of
the elicitation reaction in plant cells, as shown in the case of galacturonic acid.
Received: 18 January 1999 / Received revision: 2 April 1999 / Accepted: 1 May 1999 相似文献
80.
Localization and effects of hepatocyte growth factor on smooth muscle cells during neointimal formation after balloon denudation 总被引:3,自引:0,他引:3
Masaru Aoyagi Shinji Yamamoto Hiroshi Azuma M. Yamamoto Masashi Tamaki Yasunari Niimi Kimiyoshi Hirakawa K. Yamamoto 《Histochemistry and cell biology》1999,111(6):419-428
The migration and proliferation of smooth muscle cells (SMCs) may play a key role in tissue remodeling after arterial wall
injury. We investigated the localization and effects of hepatocyte growth factor (HGF) in rabbit carotid arteries after balloon
denudation. Immunoreactivity for HGF and the c-Met receptor was clearly observed in neointimal SMCs. The immunoreactivity
was not restricted to proliferating cells but was seen even in non-dividing cells in the basal layer of the neointima 4 and
6 weeks after balloon denudation. The distribution of platelet-derived growth factor (PDGF)-positive cells paralleled that
of proliferating SMCs. The SMCs in the basal layer of the neointima at 4 and 6 weeks were positive for matrix metalloproteinase
(MMP)-2 and membrane type 1-MMP which can activate the proform of MMP-2. HGF significantly stimulated the migration but not
proliferation of cultured SMCs. Our results suggest that HGF and PDGF act in coordination to promote the proliferation and
migration of SMCs in the earlier phases of neointimal formation and that HGF as well as MMP-2 contribute to the later stages
by facilitating the migration but not replication of SMCs.
Accepted: 19 March 1999 相似文献