全文获取类型
收费全文 | 3512篇 |
免费 | 227篇 |
国内免费 | 1篇 |
出版年
2022年 | 20篇 |
2021年 | 38篇 |
2020年 | 20篇 |
2019年 | 23篇 |
2018年 | 56篇 |
2017年 | 44篇 |
2016年 | 61篇 |
2015年 | 101篇 |
2014年 | 108篇 |
2013年 | 213篇 |
2012年 | 192篇 |
2011年 | 180篇 |
2010年 | 123篇 |
2009年 | 121篇 |
2008年 | 175篇 |
2007年 | 225篇 |
2006年 | 201篇 |
2005年 | 179篇 |
2004年 | 181篇 |
2003年 | 183篇 |
2002年 | 177篇 |
2001年 | 102篇 |
2000年 | 82篇 |
1999年 | 78篇 |
1998年 | 52篇 |
1997年 | 48篇 |
1996年 | 26篇 |
1995年 | 38篇 |
1994年 | 30篇 |
1993年 | 22篇 |
1992年 | 48篇 |
1991年 | 63篇 |
1990年 | 60篇 |
1989年 | 69篇 |
1988年 | 46篇 |
1987年 | 47篇 |
1986年 | 31篇 |
1985年 | 33篇 |
1984年 | 13篇 |
1983年 | 16篇 |
1982年 | 18篇 |
1981年 | 22篇 |
1980年 | 13篇 |
1979年 | 16篇 |
1978年 | 13篇 |
1977年 | 14篇 |
1975年 | 13篇 |
1974年 | 25篇 |
1972年 | 11篇 |
1967年 | 10篇 |
排序方式: 共有3740条查询结果,搜索用时 15 毫秒
151.
The presence of α-ketoglutarate (α-KG) dehydrogenase complex in the glutamate-producing bacteria was demonstrated for the first time with Brevibacterium flavum. The partially purified enzyme, which was specific to KG and NAD+ with the usual requirements for other co-factors, was labile and stabilized by glycerol, Mg2+, and thiamine pyrophosphate. The enzyme showed an optimum pH of 7.6 and Kms of 80, 86, and 61 μm for KG, NAD+, and CoA, respectively, cis-Aconitate, succinyl-CoA, NADPH, NADH, pyruvate, and oxalacetate strongly inhibited the activity, while it was activated by acetyl-CoA, but not by AMP. Various inorganic and organic salts also inhibited the activity. When cells were cultured in glucose and acetate media, the specific activity of the cell extracts increased markedly and reached to a maximum at the late-logarithmic phase. Then, it decreased to the basal level. The addition of glutamate stimulated the synthesis of the enzyme. 相似文献
152.
Tetsu Ando Osamu Saito Koshi Arai Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(11):2643-2649
The female sex pheromone of the oriental corn borer, Ostrinia furnacalis Guenée, was presumed to be composed of (Z)-12-tetradecenyl acetate and its geometrical isomer using electroantennogram technique. From the extracts of female moths, the presence of these compounds in a ratio of ca. 3:2 was confirmed by gas-liquid chromatography and gas-liquid chromatography combined with mass spectrometry in selected ion monitoring mode. Since the male moths were not attracted to mixtures of the two synthetic compounds, the presence of minor component(s) was suggested. 相似文献
153.
The enantioselective hydrolysis of (R,S)-3-acetoxymethyl-7,8-difluoro-2,3-dihydro-4H-[1,4]benzoxazine (I) with enzymes was investigated. Optically active I and its hydrolyzate, 7,8-difluoro-2,3-dihydro-3-hydroxymethyl-4H-[1,4]benzoxazine (II), are the intermediates for preparing optically active ofloxacins, whose racemate is known to be an excellent antibacterial agent. Lipoprotein lipase from Pseudomonas fluorescens (LPL Amano 3) was found to predominantly hydrolyze (S)-I, giving (R)-I in 54% e.e. and (R)-II in 44% e.e. On the other hand, lipase from Candida cylindracea was found to predominantly hydrolyze (R)-I, giving (S)-I in 24% e.e. and (S)-II in 20% e.e. Since, the optical purities of I and II thus obtained were not particularly high, these optically active I and II were converted into 3-acetoxymethyl-7,8-difluoro-2,3-dihydro-4-(3,5-dinitrobenzoyl)-4H-[1,4]benzoxazine (IV). After recrystallizing IV from ethyl acetate-hexane, (S)- and (R)-II were obtained with high enantiomeric excess by removing the crystallized racemic IV and subsequently hydrolyzing the resulting optically active IV with alkali. The reduction of II afforded 7,8-difluoro-2,3-dihydro-3-methyl-4H-[1,4]benzoxazine (III), for which the optical purity was estimated to be >96%e.e. by HPLC analysis. (R)- and (S)-ofloxacin were prepared from (R)- and (S)-III with retention of their configuration. 相似文献
154.
155.
Masao Fujimaki Soichi Arai Norimasa Kirigaya Yosito Sakurai 《Bioscience, biotechnology, and biochemistry》2013,77(9):855-863
Aliphatic carbonyl compounds in soybean were studied. Volatile carbonyl compounds in defatted soybean flour were identified as methanal, ethanal, n-hexanal, 2-propanone, 2- pentanone, 2-heptanone, 2-heptenal, and 2,4-decadienal, while those in raw soybean as ethanal, n-hexanal, and 2-propanone. Four kinds of non-volatile carbonyl compounds were found in defatted soybean, two of which seemed to be carbonyl ester and carbonylic acid. It is probable that the compounds in defatted soybean are mostly the secondary products derived from autoxidation of the residual fatty acids and esters in the defatting process and/or during the storage thereafter. n-Hexanal in raw soybean amounts to approximately 10 p.p.m., which is, owing to its extremely low flavoring threshold, likely to be one of the main components of the green bean flavor. 相似文献
156.
Soichi Arai Makoto Abe Michiko Yamashita Hiromichi Kato Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(4):552-559
An indole derivative having blue fluorescence was produced in cooked soybean digested at 37°C for 24 hr with an acid proteinase Molsin (optimum pH: 2.8) from Aspergillus saitoi or a usual acid proteinase pepsin (optimum pH: 1.6) from beef stomach. This indole derivative was identical with a condensation product from l-tryptophan and n-hexanal. Based on MS, NMR, IR and UV spectrometry, the condensation product was identified as l-pentyl-2, 3, 4, 9-tetrahydro-lH-pyrido [3, 4-b]-indole-3-carboxylic acid [trivial name: 1-pentyl-l, 2, 3, 4-tetrahydro-2-carboline carboxylic acid-(3)].Data were presented of the formation of the above indole derivative and of the resulting consumption of l-tryptophan and n-hexanal.The possible ocurrence of the formation of Harmala alkaloids, i.e. 2-carboline derivatives, through in vitro digestion of soybean with acid proteinases was discussed.A carbonyl-trapping ability of l-tryptophan was suggested. 相似文献
157.
Soichi Arai Masatoshi Noguchi Michiko Yamashita Hiromichi Kato Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(9):1338-1345
Soy proteins were incubated with a microbial acid protease (Molsin) under the following condition: substrate concentration, 1%; enzyme-substrate ratio (by weight), 1/100; pH, 2.8; and temperature, 40°C—flavor components and related impurities are removable from crude soy-protein concentrates by their incubation for 2 hr under the above condition. The acid-precipitated fraction of soy protein incubated for 2 hr with Molsin (i.e. 2 hr-proteolyzate) showed the following composition: 10% trichloroacetic acid (TCA) insoluble fraction, 47.52%; 10% TCA soluble peptide fraction, 52.02%; and free amino acid fraction, 0.46%. Gel filtration of the 2 hr-proteolyzate gave an elution pattern showing its molecular weight distribution.In the process of the incubation of the acid-precipitated protein, the 10% TCA insoluble fraction showed increase in amino nitrogen content, its solubility in a phosphate buffer increased to change at 6 hr, and a hydrophobic amino acid share in this fraction increased gradually.In vitro digestibility of the acid-precipitated fraction were improved and the lipoxygenase activity in this fraction decreased through the Molsin treatment.Ultracentrifugal analysis showed a decreasing tendency of the cold-insoluble fraction of soy protein during its incubation with Molsin. Optical rotatory dispersion and circular dichroism study elucidated conformational changes in this fraction during its incubation either with or without Molsin. 相似文献
158.
Michiko Yamashita Soichi Arai Shin-ya Tanimoto Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(4):953-954
An investigation was conducted on myosin and actin-activated heavy meromyosin (HMM) ATPase activities in normal porcine muscle stored for varying periods of time after death. Studies were also made on temperature dependent myosin ATPase, initial burst of ATPase and actin-activated HMM ATPase in normal and in pale, soft and exudative (PSE) porcine muscle. The maximum velocity of acto-HMM ATPase of normal muscle decreased considerably with postmortem time, while the apparent dissociation constant decreased slightly. The maximum velocity of acto-HMM ATPase of postmortem normal muscle was approximately two-times larger than that of the corresponding PSE muscle. However, almost no difference was found in the apparent dissociation constant. The size of the initial burst of phosphate-liberation of myosin prepared from normal muscle was approximately 1.2 mol/mol of myosin and from PSE muscle 0. It is assumed that the lack of contractility of PSE muscle was brought about by two basic myosin malfunctions: one, the irreversible binding of myosin to actin filament and the other, the functional damage of myosin ATPase, responsible for the formation of phosphorylated complex, even when dissociable. 相似文献
159.
Soichi Arai Hideki Suzuki Masao Fujimaki Yosito Sakurai 《Bioscience, biotechnology, and biochemistry》2013,77(4):364-369
Ethanol (1:1) extract of defatted soybean flour was fractionated systematically and the resulting phonolic acid fraction was investigated. This fraction had strong phenol-like flavor and contained at least seven phenolic acids including syringic, vanillic, ferulic, gentisic, salicylic, p-coumaric, and p-hydroxybenzoic acids. The main component among these was syringic acid, which was isolated as 3,5-dinitrobenzoate.In addition, two isomers of chlorogenic acids, presumably isochlorogenic and chlorogenic acids approximately in a ratio of 1 : 10, were found in this extract. These substances have sour, bitter and astringent flavors. 相似文献
160.
Kenji Aoki Motoo Arai Yasuji Minoda Koichi Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(12):1913-1920
The Acid-stable α-amylase and the acid-unstable α-amylase from Aspergillus niger contained one mole of sulfhydryl group per one mole of enzyme, which probably existed correlating with calcium atom that was essential for the amylase activity.Iodine reacted at acidic pH specifically with the sulfhydryl group of both enzymes and oxidized it to considerably high degree, since about 4 eq of iodine per mole of sulfhydryl group of both enzymes were consumed. The modification of the sulfhydryl group of the acid-stable α-amylase did not affect the amylase acitvity, while, that of the acid-unstable α-amylase reduced it to 70 per cents intact enzyme. It was difficult to carry out carboxy-methylation of the sulfhydryl group of the acid-stable α-amylase under mild conditions maintaining its activity, but that of the acid-unstable α-amylase was easily achieved.These facts suggested that some differences existed in the neighborhood of the sulfhydryl group of both enzymes, and that the sulfhydryl group of them was not the active site. 相似文献