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81.
Ashley R. Hedrick Daniel U. Greene Erin L. Lewis Andrew S. Hood John B. Iverson 《Ecology and evolution》2021,11(3):1225
A frequent response of organisms to climate change is altering the timing of reproduction, and advancement of reproductive timing has been a common reaction to warming temperatures in temperate regions. We tested whether this pattern applied to two common North American turtle species over the past three decades in Nebraska, USA. The timing of nesting (either first date or average date) of the Common Snapping Turtle (Chelydra serpentina) was negatively correlated with mean December maximum temperatures of the preceding year and mean May minimum and maximum temperatures in the nesting year and positively correlated with precipitation in July of the previous year. Increased temperatures during the late winter and spring likely permit earlier emergence from hibernation, increased metabolic rates and feeding opportunities, and accelerated vitellogenesis, ovulation, and egg shelling, all of which could drive earlier nesting. However, for the Painted Turtle (Chrysemys picta), the timing of nesting was positively correlated with mean minimum temperatures in September, October, December of the previous year, February of the nesting year, and April precipitation. These results suggest warmer fall, and winter temperature may impose an increased metabolic cost to painted turtles that impedes fall vitellogenesis, and April rains may slow the completion of vitellogenesis through decreased basking opportunities. For both species, nest deposition was highly correlated with body size, and larger females nested earlier in the season. Although average annual ambient temperatures have increased over the last four decades of our overall fieldwork at our study site, spring temperatures have not yet increased, and hence, nesting phenology has not advanced at our site for Chelydra. While Chrysemys exhibited a weak trend toward later nesting, this response was likely due to increased recruitment of smaller females into the population due to nest protection and predator control (Procyon lotor) in the early 2000s. Should climate change result in an increase in spring temperatures, nesting phenology would presumably respond accordingly, conditional on body size variation within these populations. 相似文献
82.
Seascape-scale trophic links for fish on inshore coral reefs 总被引:2,自引:0,他引:2
Jean P. Davis Kylie A. Pitt Brian Fry Andrew D. Olds Rod M. Connolly 《Coral reefs (Online)》2014,33(4):897-907
It is increasingly accepted that coastal habitats such as inshore coral reefs do not function in isolation but rather as part of a larger habitat network. In the Caribbean, trophic subsidies from habitats adjacent to coral reefs support the diet of reef fishes, but it is not known whether similar trophic links occur on reefs in the Indo-Pacific. Here, we test whether reef fishes in inshore coral, mangrove, and seagrass habitats are supported by trophic links. We used carbon stable isotopes and mathematical mixing models to determine the minimum proportion of resources from mangrove or seagrass habitats in the diet of five fish species from coral reefs at varying distances (0–2,200 m) from these habitats in Moreton Bay, Queensland, eastern Australia. Of the fish species that are more abundant on reefs near to mangroves, Lutjanus russelli and Acanthopagrus australis showed no minimum use of diet sources from mangrove habitat. Siganus fuscescens utilized a minimum of 25–44 % mangrove sources and this contribution increased with the proximity of reefs to mangroves (R 2 = 0.91). Seagrass or reef flat sources contributed a minimum of 14–78 % to the diet of Diagramma labiosum, a species found in higher abundance on reefs near seagrass beds, but variation in diet among reefs was unrelated to seascape structure. Seagrass or reef flat sources also contributed a minimum of 8–55 % to a fish species found only on reefs (Pseudolabrus guentheri), indicating that detrital subsidies from these habitats may subsidize fish diet on reefs. These results suggest that carbon sources from multiple habitats contribute to the functioning of inshore coral reef ecosystems and that trophic connectivity between reefs and mangroves may enhance production of a functionally important herbivore. 相似文献
83.
A developmentally regulated hydroxyproline-rich glycoprotein in maize pericarp cell walls 总被引:9,自引:3,他引:9 下载免费PDF全文
We have studied the accumulation of peptidyl hydroxyproline in the pericarp of developing maize (Zea mays L., Golden cross Bantam sweet corn) kernels. Although this hydroxyproline accumulates throughout development, it is most soluble and its content per milligram dry weight greatest at midmaturation stages of development. Salt-soluble proteins containing this hydroxyproline from isolated cell walls of developing kernels were fractionated on a CsCl density gradient and on a Chromatofocusing column, resulting in the purification of an hydroxyproline-rich glycoprotein, PC-1. PC-1 is a basic protein of approximately 65 to 70 kilodaltons in molecular weight with an isoelectric point of at least 10.2 and a density of 1.38 to 1.39 in CsCl. Amino acid composition data indicate that it is rich in hydroxyproline, threonine, proline, lysine, and glycine. Its relation to dicot extensin is discussed. 相似文献
84.
Ankareddi I Bailey MM Brazel CS Rasco JF Hood RD 《Birth defects research. Part B, Developmental and reproductive toxicology》2008,83(2):112-116
BACKGROUND: Although polymers and hydrogels are used successfully in biomedical applications, including implants and drug delivery devices, smaller molecular weight oligomers, such as those investigated here, have not been extensively studied in vivo. Poly(N‐isopropylacrylamide‐co‐acrylamide), or P(NIPAAm‐co‐AAm), has a unique thermoresponsive behavior and is under investigation as a novel drug delivery system for metastatic cancer treatment. To date, no studies have been published regarding the safety of P(NIPAAm‐co‐AAm) to the conceptus. METHODS: From gestation days (GD) 6–16, pregnant CD‐1 mice were dosed via i.p. injection with aqueous solutions containing 500, 750, or 1,000 mg/kg/d P(NIPAAm‐co‐AAm). Dams were sacrificed on GD 17 and their litters were examined for abnormalities. RESULTS: P(NIPAAm‐co‐AAm) caused no statistically significant difference in maternal weight gain or percent resorbed or dead fetuses compared to control values, but fetal weight was significantly decreased in the two highest dosage groups. CONCLUSIONS: At the highest dosages employed, maternal exposure to P(NIPAAm‐co‐AAm) was associated with decreased fetal weight. However, as the estimated human exposure levels for persons using this system would be some 1,500‐fold lower than the lowest dosage administered in this study, the authors feel that this oligomer was not shown to pose a biologically significant risk at relevant human dosages. Birth Defects Res (Part B), 2008. © 2008 Wiley‐Liss, Inc. 相似文献
85.
Spawning salmon deliver nutrients (salmon-derived nutrients, SDN) to natal watersheds that can be incorporated into terrestrial
and aquatic food webs, potentially increasing ecosystem productivity. Peterson Creek, a coastal watershed in southeast Alaska
that supports several species of anadromous fish, was sampled over the course of a storm during September 2006 to test the
hypothesis that stormflows re-introduce stored SDN into the stream. We used stable isotopes and PARAFAC modeling of fluorescence
excitation–emission spectroscopy to detect flushing of DOM from salmon carcasses in the riparian zone back into a spawning
stream. During the early storm hydrograph, streamwater concentrations of NH4–N and total dissolved phosphorus (TDP), the fluorescent protein tyrosine and the δ15N content of DOM peaked, followed by a rapid decrease during maximum stormflow. Although δ15N has previously been used to track SDN in riparian zones, the use of fluorescence spectroscopy provides an independent indicator
that SDN are being returned from the riparian zone to the stream after a period of intermediate storage outside the stream
channel. Our findings further demonstrate the utility of using both δ15N of streamwater DOM and fluorescence spectroscopy with PARAFAC modeling to monitor how the pool of streamwater DOM changes
in spawning salmon streams. 相似文献
86.
The perhumid coastal temperate rainforest (PCTR) of southeast Alaska has some of the densest soil organic carbon (SOC) stocks in the world (>300 Mg C ha?1) but the fate of this SOC with continued warming remains largely unknown. We quantified dissolved organic carbon (DOC) and carbon dioxide (CO2) yields from four different wetland types (rich fen, poor fen, forested wetland and cedar wetland) using controlled laboratory incubations of surface (10 cm) and subsurface (25 cm) soils incubated at 8 and 15 °C for 37 weeks. Furthermore, we used fluorescence characterization of DOC and laboratory bioassays to assess how climate-induced soil warming may impact the quality and bioavailability of DOC delivered to fluvial systems. Soil temperature was the strongest control on SOC turnover, with wetland type and soil depth less important in controlling CO2 flux and extractable DOC. The high temperature incubation increased average CO2 yield by ~40 and ~25% for DOC suggesting PCTR soils contain a sizeable pool of readily biodegradable SOC that can be mineralized to DOC and CO2 with future climate warming. Fluxes of CO2 were positively correlated to both extractable DOC and percent bioavailable DOC during the last few months of the incubation suggesting mineralization of SOC to DOC is a strong control of soil respiration rates. Whether the net result is increased export of either carbon form will depend on the balance between the land to water transport of DOC and the ability of soil microbial communities to mineralize DOC to CO2. 相似文献
87.
Hugh A. L. Henry Stephen D. Murphy Mhairi L. McFarlane Heather Barna Katelyn Dunning Alexandra Hood Jill C. Crosthwaite 《Restoration Ecology》2019,27(2):457-465
While best practices for evaluating restoration ecology projects are emerging rapidly, budget constraints often limit postrestoration monitoring, which emphasizes the need for practical and efficient monitoring strategies. We examined the postrestoration outcome for an ENGO (Nature Conservancy of Canada) project, to assess retroactively how variation in intensity and frequency of sampling would have affected estimates of plant species composition, diversity, and richness over time. The project restored four habitat types (mesic forest, oak woodland, wet meadow, and sand barren) using sculptured seeding of tallgrass prairie and woody species. Species‐level plant cover was monitored annually for 10 years in 168 2 × 2–m quadrats. We performed randomization tests to examine estimates of species diversity and richness as a function of the number of quadrats sampled, and assessed the necessity of annual sampling for describing changes in species composition and successional trajectories. The randomization tests revealed that sampling 10–17 quadrats, depending on habitat type, was sufficient to obtain estimates of species diversity that were at least 95% of values obtained from the whole dataset. Species richness as a function of number of quadrats sampled did not plateau, which suggests that rather than increasing the number of sampling quadrats, richness could be estimated more efficiently using nonquadrat based sampling techniques. Nonmetric multidimensional scaling analysis revealed that plant species composition largely stabilized by 3–5 years postrestoration depending on habitat type. By that time, native, seeded species dominated the restoration, and the benefits of annual sampling for tracking changes in species composition diminished. 相似文献
88.
89.
Twelkmeyer B Burström PK Li J Richard Moxon E Hood DW Schweda EK 《Carbohydrate research》2011,(13):1885-1897
The structure of lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strains 1008 and 1247 has been investigated by mass spectrometry and NMR analyses on O-deacylated LPS and core oligosaccharide material. Both strains express the conserved triheptosyl inner core, [l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-l-α-d-Hepp-(1→5)-[PPEtn→4]-α-Kdo-(2→6)-Lipid A] with PCho→6)-β-d-Glcp (GlcI) substituting the proximal heptose (HepI) at O-4. Strain 1247 expresses the common structural motifs of H. influenzae; globotetraose [β-d-GalpNAc-(1→3)-α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and its truncated versions globoside [α-d-Galp-(1→4)-β-d-Galp-(1→4)-β-d-Glcp-(1→] and lactose [β-d-Galp-(1→4)-β-d-Glcp-(1→] linked to the terminal heptose of the inner core and GlcI. A genetically distinct NTHi strain, 1008, expresses identical structures to strain 1247 with the exception that it lacks GalNAc. A lpsA mutant of strain 1247 expressed LPS of reduced complexity that facilitated unambiguous structural determination of the oligosaccharide from HepI. By CE-ESI-MS/MS we identified disialylated glycoforms indicating disialyllactose [α-Neu5Ac-(2→8)-α-Neu5Ac-(2→3)-β-d-Gal-(1→4)-β-d-Glcp-(1→] as an extension from GlcI which is a novel finding for NTHi LPS. 相似文献
90.
Sousa PM Silva ST Hood BL Charro N Carita JN Vaz F Penque D Conrads TP Melo AM 《Biochimie》2011,93(3):418-425
The organization of respiratory chain complexes in supercomplexes has been shown in the mitochondria of several eukaryotes and in the cell membranes of some bacteria. These supercomplexes are suggested to be important for oxidative phosphorylation efficiency and to prevent the formation of reactive oxygen species.Here we describe, for the first time, the identification of supramolecular organizations in the aerobic respiratory chain of Escherichia coli, including a trimer of succinate dehydrogenase. Furthermore, two heterooligomerizations have been shown: one resulting from the association of the NADH:quinone oxidoreductases NDH-1 and NDH-2, and another composed by the cytochrome bo3 quinol:oxygen reductase, cytochrome bd quinol:oxygen reductase and formate dehydrogenase (fdo). These results are supported by blue native-electrophoresis, mass spectrometry and kinetic data of wild type and mutant E . coli strains. 相似文献