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Heat and drought adaptive QTL in a wheat population designed to minimize confounding agronomic effects 总被引:3,自引:0,他引:3
R. Suzuky Pinto Matthew P. Reynolds Ky L. Mathews C. Lynne McIntyre Juan-Jose Olivares-Villegas Scott C. Chapman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(6):1001-1021
A restricted range in height and phenology of the elite Seri/Babax recombinant inbred line (RIL) population makes it ideal
for physiological and genetic studies. Previous research has shown differential expression for yield under water deficit associated
with canopy temperature (CT). In the current study, 167 RILs plus parents were phenotyped under drought (DRT), hot irrigated
(HOT), and temperate irrigated (IRR) environments to identify the genomic regions associated with stress-adaptive traits.
In total, 104 QTL were identified across a combination of 115 traits × 3 environments × 2 years, of which 14, 16, and 10 QTL
were associated exclusively with DRT, HOT, and IRR, respectively. Six genomic regions were related to a large number of traits,
namely 1B-a, 2B-a, 3B-b, 4A-a, 4A-b, and 5A-a. A yield QTL located on 4A-a explained 27 and 17% of variation under drought
and heat stress, respectively. At the same location, a QTL explained 28% of the variation in CT under heat, while 14% of CT
variation under drought was explained by a QTL on 3B-b. The T1BL.1RS (rye) translocation donated by the Seri parent was associated
with decreased yield in this population. There was no co-location of consistent yield and phenology or height-related QTL,
highlighting the utility of using a population with a restricted range in anthesis to facilitate QTL studies. Common QTL for
drought and heat stress traits were identified on 1B-a, 2B-a, 3B-b, 4A-a, 4B-b, and 7A-a confirming their generic value across
stresses. Yield QTL were shown to be associated with components of other traits, supporting the prospects for dissecting crop
performance into its physiological and genetic components in order to facilitate a more strategic approach to breeding. 相似文献
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Nan Li Peter Yun Mangala A. Nadkarni Nazila Babapoor Ghadikolaee Ky‐Anh Nguyen Mihwa Lee Neil Hunter Charles A. Collyer 《Molecular microbiology》2010,76(4):861-873
Porphyromonas gingivalis is an obligately anaerobic bacterium recognized as an aetiological agent of adult periodontitis. P. gingivalis produces cysteine proteinases, the gingipains. The crystal structure of a domain within the haemagglutinin region of the lysine gingipain (Kgp) is reported here. The domain was named K2 as it is the second of three homologous structural modules in Kgp. The K2 domain structure is a ‘jelly‐roll’ fold with two anti‐parallel β‐sheets. This fold topology is shared with adhesive domains from functionally diverse receptors such as MAM domains, ephrin receptor ligand binding domains and a number of carbohydrate binding modules. Possible functions of K2 were investigated. K2 induced haemolysis of erythrocytes in a dose‐dependent manner that was augmented by the blocking of anion transport. Further, cysteine‐activated arginine gingipain RgpB, which degrades glycophorin A, sensitized erythrocytes to the haemolytic effect of K2. Cleaved K2, similar to that found in extracted Kgp, lacks the haemolytic activity indicating that autolysis of Kgp may be a staged process which is artificially enhanced by extraction of the protein. The data indicate a functional role for K2 in the integrated capacity conferred by Kgp to enable the porphyrin auxotroph P. gingivalis to capture essential haem from erythrocytes. 相似文献
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Creyghton MP Markoulaki S Levine SS Hanna J Lodato MA Sha K Young RA Jaenisch R Boyer LA 《Cell》2008,135(4):649-661
Elucidating how chromatin influences gene expression patterns and ultimately cell fate is fundamental to understanding development and disease. The histone variant H2AZ has emerged as a key regulator of chromatin function and plays an essential but unknown role during mammalian development. Here, genome-wide analysis reveals that H2AZ occupies the promoters of developmentally important genes in a manner that is remarkably similar to that of the Polycomb group (PcG) protein Suz12. By using RNAi, we demonstrate a role for H2AZ in regulating target gene expression, find that H2AZ and PcG protein occupancy is interdependent at promoters, and further show that H2AZ is necessary for ES cell differentiation. Notably, H2AZ occupies a different subset of genes in lineage-committed cells, suggesting that its dynamic redistribution is necessary for cell fate transitions. Thus, H2AZ, together with PcG proteins, may establish specialized chromatin states in ES cells necessary for the proper execution of developmental gene expression programs. 相似文献
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Raju Rajasabapathy Chellandi Mohandass Syed Gulam Dastager Qing Liu Thi-Nhan Khieu Chu Ky Son Wen-Jun Li Ana Colaco 《Antonie van Leeuwenhoek》2014,105(3):571-578
A Gram-negative, motile, non-spore forming, rod shaped aerobic bacterium, designated strain SSW084T, was isolated from a surface seawater sample collected at Espalamaca (38°33′N; 28°39′W), Azores. Growth was found to occur from 15 to 40 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 7.0) and with 25–100 % seawater or 0.5–7.0 % NaCl in the presence of Mg2+ and Ca2+; no growth was found with NaCl alone. Colonies on seawater nutrient agar were observed to be punctiform, white, convex, circular, smooth, and translucent. Strain SSW084T did not grow on Zobell marine agar and tryptic soy agar even when seawater supplemented. The major respiratory quinone was found to be Q-10 and the G + C content was determined to be 61.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SSW084T belongs to the genus Roseovarius and that its closest neighbours are Roseovarius tolerans EL-172T, Roseovarius mucosus DFL-24T and Roseovarius lutimaris 112T with 95.7, 95.4 and 95.3 % sequence similarity respectively. The remaining species of Roseovarius showed <95 % similarity. The polar lipids of strain SSW084T were determined to be phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified lipid and one unidentified aminolipid. The major fatty acids identified were identified as C18:1 ω7c (52.5 %) and C16:0 (13.8 %). On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain SSW084T is considered to represent a novel species of the genus Roseovarius, for which Roseovarius azorensis sp. nov is proposed. The type strain is SSW084T (=KCTC 32421T = MTCC 11812T). 相似文献
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Marta S. Lopes Matthew P. Reynolds C. Lynne McIntyre Ky L. Mathews M. R. Jalal Kamali Moussa Mossad Yousef Feltaous Izzat S. A. Tahir Ravish Chatrath Francis Ogbonnaya Michael Baum 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(4):971-984
Heat and drought adaptive quantitative trait loci (QTL) in a spring bread wheat population resulting from the Seri/Babax cross designed to minimize confounding agronomic traits have been identified previously in trials conducted in Mexico. The same population was grown across a wide range of environments where heat and drought stress are naturally experienced including environments in Mexico, West Asia, North Africa (WANA), and South Asia regions. A molecular genetic linkage map including 475 marker loci associated to 29 linkage groups was used for QTL analysis of yield, days to heading (DH) and to maturity (DM), grain number (GM2), thousand kernel weight (TKW), plant height (PH), canopy temperature at the vegetative and grain filling stages (CTvg and CTgf), and early ground cover. A QTL for yield on chromosome 4A was confirmed across several environments, in subsets of lines with uniform allelic expression of a major phenology QTL, but not independently from PH. With terminal stress, TKW QTL was linked or pleiotropic to DH and DM. The link between phenology and TKW suggested that early maturity would favor the post—anthesis grain growth periods resulting in increased grain size and yields under terminal stress. GM2 and TKW were partially associated with markers at different positions suggesting different genetic regulation and room for improvement of both traits. Prediction accuracy of yield was improved by 5 % when using marker scores of component traits (GM2 and DH) together with yield in multiple regression. This procedure may provide accumulation of more favorable alleles during selection. 相似文献