全文获取类型
收费全文 | 1329篇 |
免费 | 113篇 |
国内免费 | 2篇 |
出版年
2022年 | 14篇 |
2021年 | 31篇 |
2020年 | 7篇 |
2019年 | 15篇 |
2018年 | 26篇 |
2017年 | 26篇 |
2016年 | 33篇 |
2015年 | 53篇 |
2014年 | 71篇 |
2013年 | 83篇 |
2012年 | 96篇 |
2011年 | 107篇 |
2010年 | 57篇 |
2009年 | 55篇 |
2008年 | 77篇 |
2007年 | 76篇 |
2006年 | 62篇 |
2005年 | 55篇 |
2004年 | 64篇 |
2003年 | 55篇 |
2002年 | 42篇 |
2001年 | 21篇 |
2000年 | 26篇 |
1999年 | 16篇 |
1998年 | 16篇 |
1997年 | 13篇 |
1996年 | 13篇 |
1995年 | 6篇 |
1994年 | 8篇 |
1993年 | 13篇 |
1992年 | 16篇 |
1991年 | 12篇 |
1990年 | 14篇 |
1989年 | 12篇 |
1988年 | 16篇 |
1987年 | 15篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 11篇 |
1983年 | 13篇 |
1982年 | 9篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 9篇 |
1978年 | 8篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1968年 | 3篇 |
排序方式: 共有1444条查询结果,搜索用时 781 毫秒
61.
Amanda Rui En Woo Siu Kwan Sze Hwa Hwa Chung Valerie C-L Lin 《Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms》2019,1862(4):522-533
The activation functions AF1 and AF2 of nuclear receptors mediate the recruitment of coregulators in gene regulation. AF1 is mapped to the highly variable and intrinsically unstructured N terminal domain and AF2 lies in the conserved ligand binding domain. The unstructured nature of AF1 offers structural plasticity and hence functional versatility in gene regulation. However, little is known about the key functional residues of AF1 that mediates its interaction with coregulators. This study focuses on the progesterone receptor (PR) and reports the identification of K464, K481 and R492 (KKR) as the key functional residues of PR AF1. The KKR are monomethylated and function cooperatively. The combined mutations of KKR to QQQ render PR isoform B (PRB) hyperactive, whereas KKR to FFF mutations abolishes as much as 80% of PR activity. Furthermore, the hyperactive QQQ mutation rescues the loss of PR activity due to E911A mutation in AF2. The study also finds that the magnitudes of the mutational effect differ in different cell types as a result of differential effects on the functional interaction with coregulators. Furthermore, KKR provides the interface for AF1 to physically interact with p300 and SRC-1, and with AF2 at E911. Intriguingly, the inactive FFF mutant interacts strikingly stronger with both SRC-1 and AF2 than wt PRB. We propose a tripartite model to describe the dynamic interactions between AF1, AF2 and SRC-1 with KKR of AF1 and E911 of AF2 as the interface. An overly stable interaction would hamper the dynamics of disassembly of the receptor complex. 相似文献
62.
Lijuan Sun Sanjay Verma Navin Michael Siew Pang Chan Jianhua Yan Suresh Anand Sadananthan Stefan G. Camps Hui Jen Goh Priya Govindharajulu John Totman David Townsend Julian Pak‐Nam Goh Lei Sun Bernhard Otto Boehm Su Chi Lim Siew Kwan Sze Christiani Jeyakumar Henry Houchun Harry Hu S. Sendhil Velan Melvin Khee‐Shing Leow 《Obesity (Silver Spring, Md.)》2019,27(9):1434-1442
63.
64.
Byung-Ha Oh Suhng Wook Kim Seong-Eon Ryu Sang-Soo Kim Mi-Kyung Yoon Kwan Yong Choi 《Proteins》1996,24(4):514-515
The Δ5-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P212121 with unit cell dimensions of a = 36.48 Å, b = 74.30 Å, c = 96.02 Å, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 Å resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement. 相似文献
65.
Tom Z. Yuan Pankaj Garg Linya Wang Jordan R. Willis Eric Kwan Ana G Lujan Hernandez Emily Tuscano Emily N. Sever Erica Keane Cinque Soto Eric M. Mucker Mallorie E. Fouch Edgar Davidson Benjamin J. Doranz Shweta Kailasan M. Javad Aman Haoyang Li Jay W. Hooper Erica Ollmann Saphire James E. Crowe Qiang Liu Fumiko Axelrod Aaron K. Sato 《MABS-AUSTIN》2022,14(1)
66.
67.
HENRY SILVERMAN BABIKER AHMED SAMAR AJEILET SUMAIA AL‐FADIL SUHAIL AL‐AMAD HADIR EL‐DESSOUKY IBRAHIM EL‐GENDY MOHAMED EL‐GUINDI MUSTAFA EL‐NIMEIRI RANA MUZAFFAR AZZA SALEH 《Developing world bioethics》2010,10(2):70-77
To help ensure the ethical conduct of research, many have recommended educational efforts in research ethics to investigators and members of research ethics committees (RECs). One type of education activity involves multi‐day workshops in research ethics. To be effective, such workshops should contain the appropriate content and teaching techniques geared towards the learning styles of the targeted audiences. To ensure consistency in content and quality, we describe the development of a curriculum guide, core competencies and associated learning objectives and activities to help educators organize research ethics workshops in their respective institutions. The curriculum guide is divided into modular units to enable planners to develop workshops of different lengths and choose content materials that match the needs, abilities, and prior experiences of the target audiences. The content material in the curriculum guide is relevant for audiences in the Middle East, because individuals from the Middle East who participated in a Certificate Program in research ethics selected and developed the training materials (e.g., articles, powerpoint slides, case studies, protocols). Also, many of the activities incorporate active‐learning methods, consisting of group work activities analyzing case studies and reviewing protocols. The development of such a workshop training curriculum guide represents a sustainable educational resource to enhance research ethics capacity in the Middle East. 相似文献
68.
69.
70.
A technique has been developed for measuring visible absorption spectra of chlorophyll in lipid membranes. An expression is derived which enables the directions of the transition moments of the different absorption bands to be determined from polarisation data. It is found that the transition moments of the principal blue and red absorption bands of chlorophyll a make angles of 26° and 36.5° respectively with the plane of the membrane. On the assumption that these two transitions lie in the plane of the porphyrin ring and are mutually perpendicular, it may be deduced that the plane of the porphyrin ring is tilted at approx. 48° to the membrane surface. For chlorophyll b the transition moments of the blue and red bands are found to make angles of 29.5° and 36.5° with the plane of the bilayer, giving an angle of tilt of the porphyrin ring of approx. 51°.
These results are compared with measurements of dichroism in vivo. 相似文献